Restriction map for
pF1KB8165
(Linear) (Six-base) MAPSORT of: KIBB8165.seq Check: 4596 from: 1 to: 1488
KIBB8165.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AccI GT'mk_AC
Cuts at: 0 536 1488
Size: 536 952
Acc65I G'GTAC_C
Cuts at: 0 520 977 1488
Size: 520 457 511
Fragments arranged by size:
520 511 457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 760 1488
Size: 239 521 728
Fragments arranged by size:
728 521 239
AflIII A'CryG_T
Cuts at: 0 145 1031 1488
Size: 145 886 457
Fragments arranged by size:
886 457 145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1468 1488
Size: 1468 20
AlwNI CAG_nnn'CTG
Cuts at: 0 750 873 1488
Size: 750 123 615
Fragments arranged by size:
750 615 123
ApaI G_GGCC'C
Cuts at: 0 184 1488
Size: 184 1304
ApaLI G'TGCA_C
Cuts at: 0 208 1488
Size: 208 1280
AvaI C'yCGr_G
Cuts at: 0 923 1488
Size: 923 565
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 961 994 1488
Size: 961 33 494
Fragments arranged by size:
961 494 33
BamHI G'GATC_C
Cuts at: 0 1355 1488
Size: 1355 133
BanI G'GyrC_C
Cuts at: 0 76 520 609 977 1488
Size: 76 444 89 368 511
Fragments arranged by size:
511 444 368 89 76
BanII G_rGCy'C
Cuts at: 0 184 899 1093 1488
Size: 184 715 194 395
Fragments arranged by size:
715 395 194 184
BbsI GAAGACnn'nnnn_
Cuts at: 0 1424 1488
Size: 1424 64
BbvCI CC'TCA_GC
Cuts at: 0 433 1488
Size: 433 1055
BglII A'GATC_T
Cuts at: 0 323 1488
Size: 323 1165
Bme1580I G_kGCm'C
Cuts at: 0 81 184 212 612 1488
Size: 81 103 28 400 876
Fragments arranged by size:
876 400 103 81 28
BmgBI CAC'GTC
Cuts at: 0 263 1154 1488
Size: 263 891 334
Fragments arranged by size:
891 334 263
BmrI ACTGGGnnnn_n'
Cuts at: 0 520 1488
Size: 520 968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 269 412 505 1488
Size: 152 117 143 93 983
Fragments arranged by size:
983 152 143 117 93
Bpu10I CC'TnA_GC
Cuts at: 0 83 433 1488
Size: 83 350 1055
Fragments arranged by size:
1055 350 83
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 628 844 883 1488
Size: 562 66 216 39 605
Fragments arranged by size:
605 562 216 66 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 481 848 1488
Size: 481 367 640
Fragments arranged by size:
640 481 367
BsaAI yAC'GTr
Cuts at: 0 148 1488
Size: 148 1340
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1162 1192 1255 1488
Size: 1162 30 63 233
Fragments arranged by size:
1162 233 63 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 179 298 600 1488
Size: 158 21 119 302 888
Fragments arranged by size:
888 302 158 119 21
BsiHKAI G_wGCw'C
Cuts at: 0 212 899 1488
Size: 212 687 589
Fragments arranged by size:
687 589 212
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1144 1488
Size: 1144 344
Bsp1286I G_dGCh'C
Cuts at: 0 81 184 212 612 899 1093 1488
Size: 81 103 28 400 287 194 395
Fragments arranged by size:
400 395 287 194 103 81 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 881 1488
Size: 881 607
BsrBI CCG'CTC
Cuts at: 0 1353 1488
Size: 1353 135
BsrFI r'CCGG_y
Cuts at: 0 833 980 1488
Size: 833 147 508
Fragments arranged by size:
833 508 147
BssSI C'ACGA_G
Cuts at: 0 211 1488
Size: 211 1277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 843 1315 1488
Size: 843 472 173
Fragments arranged by size:
843 472 173
BstXI CCAn_nnnn'nTGG
Cuts at: 0 963 1488
Size: 963 525
BstYI r'GATC_y
Cuts at: 0 323 1301 1355 1488
Size: 323 978 54 133
Fragments arranged by size:
978 323 133 54
Bsu36I CC'TnA_GG
Cuts at: 0 380 1488
Size: 380 1108
BtgI C'CryG_G
Cuts at: 0 813 1007 1094 1488
Size: 813 194 87 394
Fragments arranged by size:
813 394 194 87
BtsI GCAGTG_nn'
Cuts at: 0 129 1488
Size: 129 1359
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1008 1043 1488
Size: 1008 35 445
Fragments arranged by size:
1008 445 35
DraIII CAC_nnn'GTG
Cuts at: 0 786 1488
Size: 786 702
EaeI y'GGCC_r
Cuts at: 0 51 1064 1156 1488
Size: 51 1013 92 332
Fragments arranged by size:
1013 332 92 51
EarI CTCTTCn'nnn_
Cuts at: 0 596 1488
Size: 596 892
EcoICRI GAG'CTC
Cuts at: 0 897 1488
Size: 897 591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 239 269 412 505 760 1488
Size: 152 87 30 143 93 255 728
Fragments arranged by size:
728 255 152 143 93 87 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 643 730 1448 1488
Size: 643 87 718 40
Fragments arranged by size:
718 643 87 40
EcoO109I rG'GnC_Cy
Cuts at: 0 138 180 648 735 1218 1488
Size: 138 42 468 87 483 270
Fragments arranged by size:
483 468 270 138 87 42
FspI TGC'GCA
Cuts at: 0 304 1488
Size: 304 1184
HaeII r_GCGC'y
Cuts at: 0 351 1488
Size: 351 1137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 1488
Size: 265 32 1191
Fragments arranged by size:
1191 265 32
KpnI G_GTAC'C
Cuts at: 0 524 981 1488
Size: 524 457 507
Fragments arranged by size:
524 507 457
MscI TGG'CCA
Cuts at: 0 1066 1158 1488
Size: 1066 92 330
Fragments arranged by size:
1066 330 92
MslI CAynn'nnrTG
Cuts at: 0 775 1488
Size: 775 713
MspA1I CmG'CkG
Cuts at: 0 30 364 807 1488
Size: 30 334 443 681
Fragments arranged by size:
681 443 334 30
NspI r_CATG'y
Cuts at: 0 780 1035 1488
Size: 780 255 453
Fragments arranged by size:
780 453 255
PciI A'CATG_T
Cuts at: 0 1031 1488
Size: 1031 457
PfoI T'CCnGG_A
Cuts at: 0 626 1488
Size: 626 862
PmlI CAC'GTG
Cuts at: 0 148 1488
Size: 148 1340
PpuMI rG'GwC_Cy
Cuts at: 0 648 735 1488
Size: 648 87 753
Fragments arranged by size:
753 648 87
PspOMI G'GGCC_C
Cuts at: 0 180 1488
Size: 180 1308
PvuII CAG'CTG
Cuts at: 0 30 364 807 1488
Size: 30 334 443 681
Fragments arranged by size:
681 443 334 30
SacI G_AGCT'C
Cuts at: 0 899 1488
Size: 899 589
SmlI C'TyrA_G
Cuts at: 0 577 643 859 898 1488
Size: 577 66 216 39 590
Fragments arranged by size:
590 577 216 66 39
StuI AGG'CCT
Cuts at: 0 95 1488
Size: 95 1393
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1418 1488
Size: 1418 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1059 1091 1488
Size: 1059 32 397
Fragments arranged by size:
1059 397 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 811 1488
Size: 811 677
XmnI GAAnn'nnTTC
Cuts at: 0 793 1488
Size: 793 695
Enzymes that do cut:
AccI Acc65I AcuI AflIII AloI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BseRI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I KpnI MscI MslI MspA1I NspI PciI
PfoI PmlI PpuMI PspOMI PvuII SacI SmlI StuI
TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI ApoI AscI AseI AsiSI AvrII BbeI BcgI
BciVI BclI BfrBI BglI BlpI BmtI BplI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI DraI DrdI EagI EciI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI Tth111I XbaI XhoI XmaI ZraI