Restriction map for
pF1KB8170
(Linear) (Six-base) MAPSORT of: KIBB8170.seq Check: 6942 from: 1 to: 1107
KIBB8170.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 271 361 452 550 742 900 1078
Size: 79 192 90 91 98 192 158 178
Cuts at: 1078 1107
Size: 29
Fragments arranged by size:
192 192 178 158 98 91 90 79
29
AfeI AGC'GCT
Cuts at: 0 595 1107
Size: 595 512
AflIII A'CryG_T
Cuts at: 0 851 1107
Size: 851 256
AhdI GACnn_n'nnGTC
Cuts at: 0 42 1107
Size: 42 1065
AleI CACnn'nnGTG
Cuts at: 0 82 1107
Size: 82 1025
AlwNI CAG_nnn'CTG
Cuts at: 0 315 461 649 801 874 1107
Size: 315 146 188 152 73 233
Fragments arranged by size:
315 233 188 152 146 73
ApaI G_GGCC'C
Cuts at: 0 346 603 639 1107
Size: 346 257 36 468
Fragments arranged by size:
468 346 257 36
ApoI r'AATT_y
Cuts at: 0 400 1107
Size: 400 707
AvaI C'yCGr_G
Cuts at: 0 638 1107
Size: 638 469
BamHI G'GATC_C
Cuts at: 0 431 1107
Size: 431 676
BanI G'GyrC_C
Cuts at: 0 176 772 1107
Size: 176 596 335
Fragments arranged by size:
596 335 176
BanII G_rGCy'C
Cuts at: 0 282 346 479 603 639 1107
Size: 282 64 133 124 36 468
Fragments arranged by size:
468 282 133 124 64 36
BbeI G_GCGC'C
Cuts at: 0 180 1107
Size: 180 927
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 1107
Size: 155 952
BbvCI CC'TCA_GC
Cuts at: 0 1102 1107
Size: 1102 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 99 133 1107
Size: 99 34 974
Fragments arranged by size:
974 99 34
BlpI GC'TnA_GC
Cuts at: 0 551 1031 1052 1107
Size: 551 480 21 55
Fragments arranged by size:
551 480 55 21
Bme1580I G_kGCm'C
Cuts at: 0 346 603 639 777 1074 1107
Size: 346 257 36 138 297 33
Fragments arranged by size:
346 297 257 138 36 33
BmgBI CAC'GTC
Cuts at: 0 423 1107
Size: 423 684
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 169 637 961 1107
Size: 163 6 468 324 146
Fragments arranged by size:
468 324 163 146 6
Bpu10I CC'TnA_GC
Cuts at: 0 1102 1107
Size: 1102 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 538 1107
Size: 211 327 569
Fragments arranged by size:
569 327 211
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 257 1107
Size: 145 112 850
Fragments arranged by size:
850 145 112
BsaHI Gr'CG_yC
Cuts at: 0 177 1107
Size: 177 930
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 517 535 634 973 1107
Size: 136 381 18 99 339 134
Fragments arranged by size:
381 339 136 134 99 18
BseYI C'CCAG_C
Cuts at: 0 255 590 596 737 1107
Size: 255 335 6 141 370
Fragments arranged by size:
370 335 255 141 6
BsiHKAI G_wGCw'C
Cuts at: 0 282 1107
Size: 282 825
Bsp1286I G_dGCh'C
Cuts at: 0 282 346 479 603 639 777 1074 1107
Size: 282 64 133 124 36 138 297 33
Fragments arranged by size:
297 282 138 133 124 64 36 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 1106 1107
Size: 620 486 1
Fragments arranged by size:
620 486 1
BsrFI r'CCGG_y
Cuts at: 0 173 1107
Size: 173 934
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1069 1098 1107
Size: 1069 29 9
Fragments arranged by size:
1069 29 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 274 1107
Size: 274 833
BstYI r'GATC_y
Cuts at: 0 206 386 431 563 1107
Size: 206 180 45 132 544
Fragments arranged by size:
544 206 180 132 45
BtgI C'CryG_G
Cuts at: 0 427 1107
Size: 427 680
BtsI GCAGTG_nn'
Cuts at: 0 89 1107
Size: 89 1018
DraIII CAC_nnn'GTG
Cuts at: 0 1069 1107
Size: 1069 38
DrdI GACnn_nn'nnGTC
Cuts at: 0 159 1107
Size: 159 948
EcoICRI GAG'CTC
Cuts at: 0 280 1107
Size: 280 827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 163 169 271 361 452 550 637
Size: 79 84 6 102 90 91 98 87
Cuts at: 637 742 900 961 1078 1107
Size: 105 158 61 117 29
Fragments arranged by size:
158 117 105 102 98 91 90 87
84 79 61 29 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 350 616 793 805 1107
Size: 350 266 177 12 302
Fragments arranged by size:
350 302 266 177 12
EcoO109I rG'GnC_Cy
Cuts at: 0 343 609 635 798 975 1023 1107
Size: 343 266 26 163 177 48 84
Fragments arranged by size:
343 266 177 163 84 48 26
EcoRI G'AATT_C
Cuts at: 0 400 1107
Size: 400 707
HaeII r_GCGC'y
Cuts at: 0 180 597 789 1107
Size: 180 417 192 318
Fragments arranged by size:
417 318 192 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 1107
Size: 28 32 1047
Fragments arranged by size:
1047 32 28
KasI G'GCGC_C
Cuts at: 0 176 1107
Size: 176 931
MslI CAynn'nnrTG
Cuts at: 0 82 1107
Size: 82 1025
MspA1I CmG'CkG
Cuts at: 0 141 646 1107
Size: 141 505 461
Fragments arranged by size:
505 461 141
NaeI GCC'GGC
Cuts at: 0 175 1107
Size: 175 932
NarI GG'CG_CC
Cuts at: 0 177 1107
Size: 177 930
NgoMIV G'CCGG_C
Cuts at: 0 173 1107
Size: 173 934
NspI r_CATG'y
Cuts at: 0 855 1107
Size: 855 252
PasI CC'CwG_GG
Cuts at: 0 573 972 1107
Size: 573 399 135
Fragments arranged by size:
573 399 135
PciI A'CATG_T
Cuts at: 0 851 1107
Size: 851 256
PfoI T'CCnGG_A
Cuts at: 0 389 1107
Size: 389 718
PpuMI rG'GwC_Cy
Cuts at: 0 609 1023 1107
Size: 609 414 84
Fragments arranged by size:
609 414 84
PspOMI G'GGCC_C
Cuts at: 0 342 599 635 1107
Size: 342 257 36 472
Fragments arranged by size:
472 342 257 36
PstI C_TGCA'G
Cuts at: 0 140 797 1107
Size: 140 657 310
Fragments arranged by size:
657 310 140
PvuII CAG'CTG
Cuts at: 0 141 1107
Size: 141 966
SacI G_AGCT'C
Cuts at: 0 282 1107
Size: 282 825
SbfI CC_TGCA'GG
Cuts at: 0 797 1107
Size: 797 310
SfcI C'TryA_G
Cuts at: 0 136 793 1107
Size: 136 657 314
Fragments arranged by size:
657 314 136
SfoI GGC'GCC
Cuts at: 0 178 1107
Size: 178 929
SmaI CCC'GGG
Cuts at: 0 640 1107
Size: 640 467
SmlI C'TyrA_G
Cuts at: 0 190 517 1107
Size: 190 327 590
Fragments arranged by size:
590 327 190
SrfI GCCC'GGGC
Cuts at: 0 640 1107
Size: 640 467
SspI AAT'ATT
Cuts at: 0 816 1107
Size: 816 291
StuI AGG'CCT
Cuts at: 0 671 1107
Size: 671 436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 703 1107
Size: 703 404
XmaI C'CCGG_G
Cuts at: 0 638 1107
Size: 638 469
Enzymes that do cut:
AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraIII DrdI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I KasI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PpuMI PspOMI
PstI PvuII SacI SbfI SfcI SfoI SmaI SmlI
SrfI SspI StuI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI
BclI BfrBI BglI BglII BmrI BmtI BplI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EaeI
EagI EarI EciI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI StyI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI