(Linear) (Six-base) MAPSORT of: KIBB8172.seq  Check: 9977  from: 1  to: 420



KIBB8172.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     166     274     420 
   Size:        166     108     146

  Fragments arranged by size: 

                 166     146     108
 
ApoI r'AATT_y

Cuts at:      0     332     420 
   Size:        332      88
 
BamHI G'GATC_C

Cuts at:      0     354     420 
   Size:        354      66
 
BanII G_rGCy'C

Cuts at:      0     321     420 
   Size:        321      99
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     178     244     420 
   Size:        178      66     176

  Fragments arranged by size: 

                 178     176      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     191     194     221     224     420 
   Size:        191       3      27       3     196

  Fragments arranged by size: 

                 196     191      27       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     215     420 
   Size:        215     205
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     420 
   Size:        321      99
 
BspHI T'CATG_A

Cuts at:      0      11     420 
   Size:         11     409
 
BsrDI GCAATG_nn'

Cuts at:      0     260     420 
   Size:        260     160
 
BstYI r'GATC_y

Cuts at:      0     354     420 
   Size:        354      66
 
BtsI GCAGTG_nn'

Cuts at:      0     212     420 
   Size:        212     208
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     261     296     420 
   Size:        261      35     124

  Fragments arranged by size: 

                 261     124      35
 
EcoRI G'AATT_C

Cuts at:      0     332     420 
   Size:        332      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     420 
   Size:        177     243
 
StyI C'CwwG_G

Cuts at:      0      32     131     420 
   Size:         32      99     289

  Fragments arranged by size: 

                 289      99      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     191     194     223     226     420 
   Size:        191       3      29       3     194

  Fragments arranged by size: 

                 194     191      29       3       3
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      72     138     420 
   Size:         72      66     282

  Fragments arranged by size: 

                 282      72      66
 
XmnI GAAnn'nnTTC

Cuts at:      0     332     420 
   Size:        332      88
 

 Enzymes that do cut:

    AlwNI     ApoI    BamHI    BanII     BbsI    BsaXI    BseRI Bsp1286I
    BspHI    BsrDI    BstYI     BtsI    CspCI    EcoRI     MmeI     StyI
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     ZraI