Restriction map for
pF1KB8174
(Linear) (Six-base) MAPSORT of: KIBB8174.seq Check: 2225 from: 1 to: 1689
KIBB8174.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
Acc65I G'GTAC_C
Cuts at: 0 45 1689
Size: 45 1644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 98 415 1586 1689
Size: 74 24 317 1171 103
Fragments arranged by size:
1171 317 103 74 24
AleI CACnn'nnGTG
Cuts at: 0 1204 1689
Size: 1204 485
AlwNI CAG_nnn'CTG
Cuts at: 0 871 1564 1689
Size: 871 693 125
Fragments arranged by size:
871 693 125
ApoI r'AATT_y
Cuts at: 0 514 722 1689
Size: 514 208 967
Fragments arranged by size:
967 514 208
BanI G'GyrC_C
Cuts at: 0 45 1689
Size: 45 1644
BanII G_rGCy'C
Cuts at: 0 546 1147 1261 1396 1689
Size: 546 601 114 135 293
Fragments arranged by size:
601 546 293 135 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 1689
Size: 86 1603
BbvCI CC'TCA_GC
Cuts at: 0 1249 1298 1606 1689
Size: 1249 49 308 83
Fragments arranged by size:
1249 308 83 49
BclI T'GATC_A
Cuts at: 0 342 350 527 1689
Size: 342 8 177 1162
Fragments arranged by size:
1162 342 177 8
BlpI GC'TnA_GC
Cuts at: 0 1390 1689
Size: 1390 299
BmrI ACTGGGnnnn_n'
Cuts at: 0 19 1198 1432 1689
Size: 19 1179 234 257
Fragments arranged by size:
1179 257 234 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1166 1689
Size: 1166 523
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1099 1245 1689
Size: 530 569 146 444
Fragments arranged by size:
569 530 444 146
Bpu10I CC'TnA_GC
Cuts at: 0 436 1091 1249 1298 1606 1689
Size: 436 655 158 49 308 83
Fragments arranged by size:
655 436 308 158 83 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1161 1689
Size: 1161 528
BsaI GGTCTCn'nnnn_
Cuts at: 0 1329 1689
Size: 1329 360
BsaBI GATnn'nnATC
Cuts at: 0 616 1689
Size: 616 1073
BsaWI w'CCGG_w
Cuts at: 0 454 1689
Size: 454 1235
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 709 826 1024 1142 1317 1630 1689
Size: 277 432 117 198 118 175 313 59
Fragments arranged by size:
432 313 277 198 175 118 117 59
BseYI C'CCAG_C
Cuts at: 0 139 1689
Size: 139 1550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 1689
Size: 595 1094
BsiHKAI G_wGCw'C
Cuts at: 0 546 1087 1396 1689
Size: 546 541 309 293
Fragments arranged by size:
546 541 309 293
Bsp1286I G_dGCh'C
Cuts at: 0 546 1087 1147 1261 1396 1689
Size: 546 541 60 114 135 293
Fragments arranged by size:
546 541 293 135 114 60
BspEI T'CCGG_A
Cuts at: 0 454 1689
Size: 454 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 508 1689
Size: 508 1181
BtgI C'CryG_G
Cuts at: 0 683 689 1223 1439 1466 1689
Size: 683 6 534 216 27 223
Fragments arranged by size:
683 534 223 216 27 6
BtsI GCAGTG_nn'
Cuts at: 0 540 1282 1689
Size: 540 742 407
Fragments arranged by size:
742 540 407
DraIII CAC_nnn'GTG
Cuts at: 0 1665 1689
Size: 1665 24
EaeI y'GGCC_r
Cuts at: 0 686 1120 1241 1463 1689
Size: 686 434 121 222 226
Fragments arranged by size:
686 434 226 222 121
EarI CTCTTCn'nnn_
Cuts at: 0 400 620 632 773 1309 1689
Size: 400 220 12 141 536 380
Fragments arranged by size:
536 400 380 220 141 12
EcoICRI GAG'CTC
Cuts at: 0 544 1394 1689
Size: 544 850 295
Fragments arranged by size:
850 544 295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 98 415 530 1099 1245 1586 1689
Size: 74 24 317 115 569 146 341 103
Fragments arranged by size:
569 341 317 146 115 103 74 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 53 1252 1689
Size: 53 1199 437
Fragments arranged by size:
1199 437 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1106 1689
Size: 1106 583
HaeII r_GCGC'y
Cuts at: 0 1387 1689
Size: 1387 302
KpnI G_GTAC'C
Cuts at: 0 49 1689
Size: 49 1640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 986 1689
Size: 125 861 703
Fragments arranged by size:
861 703 125
MscI TGG'CCA
Cuts at: 0 688 1243 1465 1689
Size: 688 555 222 224
Fragments arranged by size:
688 555 224 222
MslI CAynn'nnrTG
Cuts at: 0 739 1204 1689
Size: 739 465 485
Fragments arranged by size:
739 485 465
MspA1I CmG'CkG
Cuts at: 0 673 837 1119 1689
Size: 673 164 282 570
Fragments arranged by size:
673 570 282 164
NcoI C'CATG_G
Cuts at: 0 683 1223 1439 1466 1689
Size: 683 540 216 27 223
Fragments arranged by size:
683 540 223 216 27
PfoI T'CCnGG_A
Cuts at: 0 309 1689
Size: 309 1380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 959 991 1689
Size: 959 32 698
Fragments arranged by size:
959 698 32
PstI C_TGCA'G
Cuts at: 0 505 1689
Size: 505 1184
SacI G_AGCT'C
Cuts at: 0 546 1396 1689
Size: 546 850 293
Fragments arranged by size:
850 546 293
SapI GCTCTTCn'nnn_
Cuts at: 0 1309 1689
Size: 1309 380
SfcI C'TryA_G
Cuts at: 0 221 501 1689
Size: 221 280 1188
Fragments arranged by size:
1188 280 221
SmlI C'TyrA_G
Cuts at: 0 1140 1689
Size: 1140 549
SspI AAT'ATT
Cuts at: 0 1233 1689
Size: 1233 456
StyI C'CwwG_G
Cuts at: 0 683 1223 1439 1466 1689
Size: 683 540 216 27 223
Fragments arranged by size:
683 540 223 216 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 412 597 1203 1689
Size: 412 185 606 486
Fragments arranged by size:
606 486 412 185
TatI w'GTAC_w
Cuts at: 0 1417 1689
Size: 1417 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 305 337 1689
Size: 305 32 1352
Fragments arranged by size:
1352 305 32
Tth111I GACn'n_nGTC
Cuts at: 0 916 1689
Size: 916 773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1203 1689
Size: 1203 486
XmnI GAAnn'nnTTC
Cuts at: 0 971 1689
Size: 971 718
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApoI BanI BanII BbsI
BbvCI BclI BlpI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BtgI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KpnI MmeI MscI MslI
MspA1I NcoI PfoI PpiI PstI SacI SapI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI
BglI BglII Bme1580I BmgBI BmtI BsaAI BsaHI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XhoI XmaI ZraI