Restriction map for
pF1KB8178
(Linear) (Six-base) MAPSORT of: KIBB8178.seq Check: 1001 from: 1 to: 1305
KIBB8178.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 0 1105 1305
Size: 1105 200
Acc65I G'GTAC_C
Cuts at: 0 943 1305
Size: 943 362
AclI AA'CG_TT
Cuts at: 0 735 1305
Size: 735 570
AflIII A'CryG_T
Cuts at: 0 777 1100 1305
Size: 777 323 205
Fragments arranged by size:
777 323 205
AhdI GACnn_n'nnGTC
Cuts at: 0 436 1194 1305
Size: 436 758 111
Fragments arranged by size:
758 436 111
AlwNI CAG_nnn'CTG
Cuts at: 0 39 364 576 634 1305
Size: 39 325 212 58 671
Fragments arranged by size:
671 325 212 58 39
ApoI r'AATT_y
Cuts at: 0 55 378 1069 1305
Size: 55 323 691 236
Fragments arranged by size:
691 323 236 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 927 960 1305
Size: 927 33 345
Fragments arranged by size:
927 345 33
BamHI G'GATC_C
Cuts at: 0 787 1305
Size: 787 518
BanI G'GyrC_C
Cuts at: 0 943 1305
Size: 943 362
BanII G_rGCy'C
Cuts at: 0 1271 1305
Size: 1271 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1281 1305
Size: 1281 24
BfrBI ATG'CAT
Cuts at: 0 179 1159 1305
Size: 179 980 146
Fragments arranged by size:
980 179 146
BglI GCCn_nnn'nGGC
Cuts at: 0 571 1305
Size: 571 734
BglII A'GATC_T
Cuts at: 0 395 1305
Size: 395 910
BlpI GC'TnA_GC
Cuts at: 0 889 1305
Size: 889 416
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 592 1305
Size: 492 100 713
Fragments arranged by size:
713 492 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 454 598 1305
Size: 16 438 144 707
Fragments arranged by size:
707 438 144 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 1305
Size: 595 710
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1305
Size: 388 917
BsiHKAI G_wGCw'C
Cuts at: 0 587 1271 1305
Size: 587 684 34
Fragments arranged by size:
684 587 34
BsmI GAATG_Cn'
Cuts at: 0 181 389 883 1305
Size: 181 208 494 422
Fragments arranged by size:
494 422 208 181
Bsp1286I G_dGCh'C
Cuts at: 0 587 1271 1305
Size: 587 684 34
Fragments arranged by size:
684 587 34
BsrDI GCAATG_nn'
Cuts at: 0 326 1305
Size: 326 979
BsrGI T'GTAC_A
Cuts at: 0 239 1305
Size: 239 1066
BstAPI GCAn_nnn'nTGC
Cuts at: 0 124 1305
Size: 124 1181
BstEII G'GTnAC_C
Cuts at: 0 487 1305
Size: 487 818
BstXI CCAn_nnnn'nTGG
Cuts at: 0 727 1150 1305
Size: 727 423 155
Fragments arranged by size:
727 423 155
BstYI r'GATC_y
Cuts at: 0 395 787 1305
Size: 395 392 518
Fragments arranged by size:
518 395 392
BstZ17I GTA'TAC
Cuts at: 0 1106 1305
Size: 1106 199
Bsu36I CC'TnA_GG
Cuts at: 0 757 1305
Size: 757 548
BtsI GCAGTG_nn'
Cuts at: 0 5 125 536 1305
Size: 5 120 411 769
Fragments arranged by size:
769 411 120 5
DrdI GACnn_nn'nnGTC
Cuts at: 0 369 1305
Size: 369 936
EcoICRI GAG'CTC
Cuts at: 0 1269 1305
Size: 1269 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 454 598 1305
Size: 16 438 144 707
Fragments arranged by size:
707 438 144 16
EcoO109I rG'GnC_Cy
Cuts at: 0 554 573 679 870 1305
Size: 554 19 106 191 435
Fragments arranged by size:
554 435 191 106 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 422 454 464 496 543 575
Size: 255 32 135 32 10 32 47 32
Cuts at: 575 616 648 1180 1212 1305
Size: 41 32 532 32 93
Fragments arranged by size:
532 255 135 93 47 41 32 32
32 32 32 32 10
KpnI G_GTAC'C
Cuts at: 0 947 1305
Size: 947 358
MfeI C'AATT_G
Cuts at: 0 86 1087 1305
Size: 86 1001 218
Fragments arranged by size:
1001 218 86
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1073 1305
Size: 228 845 232
Fragments arranged by size:
845 232 228
MslI CAynn'nnrTG
Cuts at: 0 725 776 1305
Size: 725 51 529
Fragments arranged by size:
725 529 51
MspA1I CmG'CkG
Cuts at: 0 39 1193 1305
Size: 39 1154 112
Fragments arranged by size:
1154 112 39
NsiI A_TGCA'T
Cuts at: 0 181 1161 1305
Size: 181 980 144
Fragments arranged by size:
980 181 144
NspI r_CATG'y
Cuts at: 0 48 781 1104 1305
Size: 48 733 323 201
Fragments arranged by size:
733 323 201 48
PciI A'CATG_T
Cuts at: 0 777 1100 1305
Size: 777 323 205
Fragments arranged by size:
777 323 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 1092 1305
Size: 576 516 213
Fragments arranged by size:
576 516 213
PstI C_TGCA'G
Cuts at: 0 543 1305
Size: 543 762
PvuII CAG'CTG
Cuts at: 0 39 1193 1305
Size: 39 1154 112
Fragments arranged by size:
1154 112 39
SacI G_AGCT'C
Cuts at: 0 1271 1305
Size: 1271 34
SfcI C'TryA_G
Cuts at: 0 539 1305
Size: 539 766
StyI C'CwwG_G
Cuts at: 0 834 1305
Size: 834 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 583 1305
Size: 583 722
TatI w'GTAC_w
Cuts at: 0 239 1305
Size: 239 1066
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1305
Size: 573 732
XmnI GAAnn'nnTTC
Cuts at: 0 293 1305
Size: 293 1012
Enzymes that do cut:
AccI Acc65I AclI AflIII AhdI AlwNI ApoI BaeI
BamHI BanI BanII BciVI BfrBI BglI BglII BlpI
BmrI BpmI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI
BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtsI
DrdI EcoICRI Eco57MI EcoO109I Hin4I KpnI MfeI MmeI
MslI MspA1I NsiI NspI PciI PflMI PstI PvuII
SacI SfcI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AcuI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BbeI BbsI BbvCI BcgI BclI Bme1580I BmgBI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI BstBI BtgI BtgZI ClaI
CspCI DraI DraIII EaeI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI