Restriction map for
pF1KB8179
(Linear) (Six-base) MAPSORT of: KIBB8179.seq Check: 7540 from: 1 to: 1302
KIBB8179.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 893 1302
Size: 893 409
AatII G_ACGT'C
Cuts at: 0 1159 1302
Size: 1159 143
Acc65I G'GTAC_C
Cuts at: 0 1115 1302
Size: 1115 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 1264 1302
Size: 347 917 38
Fragments arranged by size:
917 347 38
AleI CACnn'nnGTG
Cuts at: 0 251 1302
Size: 251 1051
AlwNI CAG_nnn'CTG
Cuts at: 0 222 387 480 675 902 1302
Size: 222 165 93 195 227 400
Fragments arranged by size:
400 227 222 195 165 93
ApaLI G'TGCA_C
Cuts at: 0 1050 1302
Size: 1050 252
AseI AT'TA_AT
Cuts at: 0 1229 1302
Size: 1229 73
AvaI C'yCGr_G
Cuts at: 0 260 929 1205 1302
Size: 260 669 276 97
Fragments arranged by size:
669 276 260 97
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 490 523 1302
Size: 490 33 779
Fragments arranged by size:
779 490 33
BanI G'GyrC_C
Cuts at: 0 1115 1302
Size: 1115 187
BbvCI CC'TCA_GC
Cuts at: 0 769 1302
Size: 769 533
BciVI GTATCCnnnnn_n'
Cuts at: 0 267 1302
Size: 267 1035
BglII A'GATC_T
Cuts at: 0 422 911 1302
Size: 422 489 391
Fragments arranged by size:
489 422 391
BlpI GC'TnA_GC
Cuts at: 0 122 523 1302
Size: 122 401 779
Fragments arranged by size:
779 401 122
Bme1580I G_kGCm'C
Cuts at: 0 373 1054 1302
Size: 373 681 248
Fragments arranged by size:
681 373 248
BmrI ACTGGGnnnn_n'
Cuts at: 0 912 1064 1302
Size: 912 152 238
Fragments arranged by size:
912 238 152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1302
Size: 1231 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 127 287 718 781 1302
Size: 106 21 160 431 63 521
Fragments arranged by size:
521 431 160 106 63 21
Bpu10I CC'TnA_GC
Cuts at: 0 769 973 1302
Size: 769 204 329
Fragments arranged by size:
769 329 204
BsaI GGTCTCn'nnnn_
Cuts at: 0 913 1302
Size: 913 389
BsaAI yAC'GTr
Cuts at: 0 105 293 1302
Size: 105 188 1009
Fragments arranged by size:
1009 188 105
BsaBI GATnn'nnATC
Cuts at: 0 490 1021 1302
Size: 490 531 281
Fragments arranged by size:
531 490 281
BsaHI Gr'CG_yC
Cuts at: 0 935 1156 1302
Size: 935 221 146
Fragments arranged by size:
935 221 146
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 385 550 1302
Size: 103 282 165 752
Fragments arranged by size:
752 282 165 103
BseYI C'CCAG_C
Cuts at: 0 331 855 948 1302
Size: 331 524 93 354
Fragments arranged by size:
524 354 331 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 235 604 694 703 790 1302
Size: 82 153 369 90 9 87 512
Fragments arranged by size:
512 369 153 90 87 82 9
BsiHKAI G_wGCw'C
Cuts at: 0 292 323 1054 1302
Size: 292 31 731 248
Fragments arranged by size:
731 292 248 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 663 1302
Size: 663 639
Bsp1286I G_dGCh'C
Cuts at: 0 292 323 373 1054 1302
Size: 292 31 50 681 248
Fragments arranged by size:
681 292 248 50 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 857 893 1302
Size: 857 36 409
Fragments arranged by size:
857 409 36
BsrBI CCG'CTC
Cuts at: 0 78 1302
Size: 78 1224
BsrFI r'CCGG_y
Cuts at: 0 297 1302
Size: 297 1005
BsrGI T'GTAC_A
Cuts at: 0 338 1302
Size: 338 964
BstAPI GCAn_nnn'nTGC
Cuts at: 0 480 519 675 717 1109 1302
Size: 480 39 156 42 392 193
Fragments arranged by size:
480 392 193 156 42 39
BstYI r'GATC_y
Cuts at: 0 422 476 818 911 1302
Size: 422 54 342 93 391
Fragments arranged by size:
422 391 342 93 54
BtgI C'CryG_G
Cuts at: 0 1262 1302
Size: 1262 40
BtsI GCAGTG_nn'
Cuts at: 0 13 258 1054 1302
Size: 13 245 796 248
Fragments arranged by size:
796 248 245 13
DraIII CAC_nnn'GTG
Cuts at: 0 105 1125 1302
Size: 105 1020 177
Fragments arranged by size:
1020 177 105
EaeI y'GGCC_r
Cuts at: 0 128 295 488 530 1191 1302
Size: 128 167 193 42 661 111
Fragments arranged by size:
661 193 167 128 111 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 718 1135 1302
Size: 718 417 167
Fragments arranged by size:
718 417 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 127 287 347 718 781 1264 1302
Size: 106 21 160 60 371 63 483 38
Fragments arranged by size:
483 371 160 106 63 60 38 21
EcoO109I rG'GnC_Cy
Cuts at: 0 39 149 384 637 745 765 1302
Size: 39 110 235 253 108 20 537
Fragments arranged by size:
537 253 235 110 108 39 20
HaeII r_GCGC'y
Cuts at: 0 675 1006 1302
Size: 675 331 296
Fragments arranged by size:
675 331 296
KpnI G_GTAC'C
Cuts at: 0 1119 1302
Size: 1119 183
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 1190 1302
Size: 626 564 112
Fragments arranged by size:
626 564 112
MscI TGG'CCA
Cuts at: 0 130 490 1193 1302
Size: 130 360 703 109
Fragments arranged by size:
703 360 130 109
MslI CAynn'nnrTG
Cuts at: 0 251 1302
Size: 251 1051
MspA1I CmG'CkG
Cuts at: 0 114 759 813 1044 1302
Size: 114 645 54 231 258
Fragments arranged by size:
645 258 231 114 54
NaeI GCC'GGC
Cuts at: 0 299 1302
Size: 299 1003
NcoI C'CATG_G
Cuts at: 0 1262 1302
Size: 1262 40
NgoMIV G'CCGG_C
Cuts at: 0 297 1302
Size: 297 1005
NspI r_CATG'y
Cuts at: 0 522 1302
Size: 522 780
PflMI CCAn_nnn'nTGG
Cuts at: 0 888 1302
Size: 888 414
PfoI T'CCnGG_A
Cuts at: 0 608 1302
Size: 608 694
PmlI CAC'GTG
Cuts at: 0 293 1302
Size: 293 1009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 1302
Size: 400 32 870
Fragments arranged by size:
870 400 32
PpuMI rG'GwC_Cy
Cuts at: 0 39 149 637 1302
Size: 39 110 488 665
Fragments arranged by size:
665 488 110 39
PshAI GACnn'nnGTC
Cuts at: 0 635 1302
Size: 635 667
PstI C_TGCA'G
Cuts at: 0 47 485 1302
Size: 47 438 817
Fragments arranged by size:
817 438 47
PvuII CAG'CTG
Cuts at: 0 114 759 1302
Size: 114 645 543
Fragments arranged by size:
645 543 114
SanDI GG'GwC_CC
Cuts at: 0 149 1302
Size: 149 1153
SfcI C'TryA_G
Cuts at: 0 43 481 1302
Size: 43 438 821
Fragments arranged by size:
821 438 43
SmaI CCC'GGG
Cuts at: 0 931 1302
Size: 931 371
SphI G_CATG'C
Cuts at: 0 522 1302
Size: 522 780
StyI C'CwwG_G
Cuts at: 0 1262 1302
Size: 1262 40
TatI w'GTAC_w
Cuts at: 0 338 1302
Size: 338 964
Tth111I GACn'n_nGTC
Cuts at: 0 37 1302
Size: 37 1265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 311 1302
Size: 171 140 991
Fragments arranged by size:
991 171 140
XmaI C'CCGG_G
Cuts at: 0 929 1302
Size: 929 373
ZraI GAC'GTC
Cuts at: 0 1157 1302
Size: 1157 145
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AlwNI ApaLI AseI
AvaI BaeI BanI BbvCI BciVI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BstAPI BstYI BtgI BtsI DraIII EaeI
EciI Eco57MI EcoO109I HaeII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PmlI
PpiI PpuMI PshAI PstI PvuII SanDI SfcI SmaI
SphI StyI TatI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApoI AscI AsiSI AvrII BamHI BanII
BbeI BbsI BcgI BclI BfrBI BglI BmgBI BmtI
BpuEI BsaWI BsaXI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TstI XbaI XhoI XmnI