Restriction map for
pF1KB8183
(Linear) (Six-base) MAPSORT of: KIBB8183.seq Check: 7867 from: 1 to: 612
KIBB8183.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 612
Size: 422 190
AlwNI CAG_nnn'CTG
Cuts at: 0 267 612
Size: 267 345
ApaI G_GGCC'C
Cuts at: 0 49 612
Size: 49 563
AvaI C'yCGr_G
Cuts at: 0 48 97 612
Size: 48 49 515
Fragments arranged by size:
515 49 48
BanI G'GyrC_C
Cuts at: 0 28 602 612
Size: 28 574 10
Fragments arranged by size:
574 28 10
BanII G_rGCy'C
Cuts at: 0 49 97 612
Size: 49 48 515
Fragments arranged by size:
515 49 48
BbeI G_GCGC'C
Cuts at: 0 32 606 612
Size: 32 574 6
Fragments arranged by size:
574 32 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 401 612
Size: 401 211
Bme1580I G_kGCm'C
Cuts at: 0 49 612
Size: 49 563
BmgBI CAC'GTC
Cuts at: 0 399 612
Size: 399 213
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 612
Size: 581 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 507 612
Size: 507 105
BsaHI Gr'CG_yC
Cuts at: 0 29 603 612
Size: 29 574 9
Fragments arranged by size:
574 29 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 385 397 483 612
Size: 91 294 12 86 129
Fragments arranged by size:
294 129 91 86 12
BseYI C'CCAG_C
Cuts at: 0 129 612
Size: 129 483
BsiHKAI G_wGCw'C
Cuts at: 0 247 612
Size: 247 365
Bsp1286I G_dGCh'C
Cuts at: 0 49 97 247 612
Size: 49 48 150 365
Fragments arranged by size:
365 150 49 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 612
Size: 201 411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 267 612
Size: 267 345
BstEII G'GTnAC_C
Cuts at: 0 543 612
Size: 543 69
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 154 189 612
Size: 154 35 423
Fragments arranged by size:
423 154 35
EaeI y'GGCC_r
Cuts at: 0 59 612
Size: 59 553
EarI CTCTTCn'nnn_
Cuts at: 0 121 320 362 612
Size: 121 199 42 250
Fragments arranged by size:
250 199 121 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 612
Size: 88 524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 612
Size: 422 190
EcoO109I rG'GnC_Cy
Cuts at: 0 45 213 612
Size: 45 168 399
Fragments arranged by size:
399 168 45
HaeII r_GCGC'y
Cuts at: 0 32 268 606 612
Size: 32 236 338 6
Fragments arranged by size:
338 236 32 6
KasI G'GCGC_C
Cuts at: 0 28 602 612
Size: 28 574 10
Fragments arranged by size:
574 28 10
NarI GG'CG_CC
Cuts at: 0 29 603 612
Size: 29 574 9
Fragments arranged by size:
574 29 9
NspI r_CATG'y
Cuts at: 0 261 612
Size: 261 351
PpuMI rG'GwC_Cy
Cuts at: 0 213 612
Size: 213 399
PspOMI G'GGCC_C
Cuts at: 0 45 612
Size: 45 567
SfoI GGC'GCC
Cuts at: 0 30 604 612
Size: 30 574 8
Fragments arranged by size:
574 30 8
SmaI CCC'GGG
Cuts at: 0 50 612
Size: 50 562
StyI C'CwwG_G
Cuts at: 0 221 612
Size: 221 391
Tth111I GACn'n_nGTC
Cuts at: 0 553 612
Size: 553 59
XmaI C'CCGG_G
Cuts at: 0 48 612
Size: 48 564
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI
Bme1580I BmgBI BmrI BplI BsaHI BseRI BseYI BsiHKAI
Bsp1286I BspMI BstAPI BstEII CspCI EaeI EarI EciI
Eco57MI EcoO109I HaeII KasI NarI NspI PpuMI PspOMI
SfoI SmaI StyI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI XbaI XcmI XhoI XmnI ZraI