Restriction map for
pF1KB8189
(Linear) (Six-base) MAPSORT of: KIBB8189.seq Check: 6958 from: 1 to: 1338
KIBB8189.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 620 693 777 861 945 1029 1197
Size: 329 291 73 84 84 84 84 168
Cuts at: 1197 1338
Size: 141
Fragments arranged by size:
329 291 168 141 84 84 84 84
73
AleI CACnn'nnGTG
Cuts at: 0 920 1338
Size: 920 418
AlwNI CAG_nnn'CTG
Cuts at: 0 42 147 1338
Size: 42 105 1191
Fragments arranged by size:
1191 105 42
ApoI r'AATT_y
Cuts at: 0 302 549 1247 1338
Size: 302 247 698 91
Fragments arranged by size:
698 302 247 91
BamHI G'GATC_C
Cuts at: 0 827 1338
Size: 827 511
BanII G_rGCy'C
Cuts at: 0 721 889 973 1057 1338
Size: 721 168 84 84 281
Fragments arranged by size:
721 281 168 84 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 317 877 1338
Size: 317 560 461
Fragments arranged by size:
560 461 317
BclI T'GATC_A
Cuts at: 0 294 1338
Size: 294 1044
BmrI ACTGGGnnnn_n'
Cuts at: 0 346 677 761 1338
Size: 346 331 84 577
Fragments arranged by size:
577 346 331 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 452 903 1025 1109 1277 1338
Size: 262 190 451 122 84 168 61
Fragments arranged by size:
451 262 190 168 122 84 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 1338
Size: 355 983
BsaI GGTCTCn'nnnn_
Cuts at: 0 271 373 1338
Size: 271 102 965
Fragments arranged by size:
965 271 102
BsaWI w'CCGG_w
Cuts at: 0 182 1338
Size: 182 1156
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 1338
Size: 496 842
BseYI C'CCAG_C
Cuts at: 0 417 1338
Size: 417 921
BsiEI CG_ry'CG
Cuts at: 0 1313 1338
Size: 1313 25
BsiHKAI G_wGCw'C
Cuts at: 0 57 721 889 973 1057 1338
Size: 57 664 168 84 84 281
Fragments arranged by size:
664 281 168 84 84 57
Bsp1286I G_dGCh'C
Cuts at: 0 57 721 889 973 1057 1338
Size: 57 664 168 84 84 281
Fragments arranged by size:
664 281 168 84 84 57
BspEI T'CCGG_A
Cuts at: 0 182 1338
Size: 182 1156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 216 1338
Size: 216 1122
BsrDI GCAATG_nn'
Cuts at: 0 948 1338
Size: 948 390
BstEII G'GTnAC_C
Cuts at: 0 150 1338
Size: 150 1188
BstYI r'GATC_y
Cuts at: 0 827 1338
Size: 827 511
Bsu36I CC'TnA_GG
Cuts at: 0 35 1338
Size: 35 1303
BtgI C'CryG_G
Cuts at: 0 581 1338
Size: 581 757
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 930 965 1338
Size: 930 35 373
Fragments arranged by size:
930 373 35
DraI TTT'AAA
Cuts at: 0 378 1338
Size: 378 960
EarI CTCTTCn'nnn_
Cuts at: 0 62 1338
Size: 62 1276
EcoICRI GAG'CTC
Cuts at: 0 719 887 971 1055 1338
Size: 719 168 84 84 283
Fragments arranged by size:
719 283 168 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 329 452 620 693 777 861 903
Size: 262 67 123 168 73 84 84 42
Cuts at: 903 945 1025 1029 1109 1197 1277 1338
Size: 42 80 4 80 88 80 61
Fragments arranged by size:
262 168 123 88 84 84 80 80
80 73 67 61 42 42 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 963 1338
Size: 963 375
EcoO109I rG'GnC_Cy
Cuts at: 0 39 219 330 1338
Size: 39 180 111 1008
Fragments arranged by size:
1008 180 111 39
EcoRI G'AATT_C
Cuts at: 0 549 1247 1338
Size: 549 698 91
Fragments arranged by size:
698 549 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 304 336 612 644 1338
Size: 7 32 265 32 276 32 694
Fragments arranged by size:
694 276 265 32 32 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 578 1338
Size: 107 471 760
Fragments arranged by size:
760 471 107
MslI CAynn'nnrTG
Cuts at: 0 602 920 1338
Size: 602 318 418
Fragments arranged by size:
602 418 318
MspA1I CmG'CkG
Cuts at: 0 417 797 881 1338
Size: 417 380 84 457
Fragments arranged by size:
457 417 380 84
PasI CC'CwG_GG
Cuts at: 0 497 1338
Size: 497 841
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 463 1338
Size: 147 316 875
Fragments arranged by size:
875 316 147
PpuMI rG'GwC_Cy
Cuts at: 0 219 330 1338
Size: 219 111 1008
Fragments arranged by size:
1008 219 111
PvuII CAG'CTG
Cuts at: 0 797 881 1338
Size: 797 84 457
Fragments arranged by size:
797 457 84
SacI G_AGCT'C
Cuts at: 0 721 889 973 1057 1338
Size: 721 168 84 84 281
Fragments arranged by size:
721 281 168 84 84
SapI GCTCTTCn'nnn_
Cuts at: 0 62 1338
Size: 62 1276
SfcI C'TryA_G
Cuts at: 0 225 798 1134 1338
Size: 225 573 336 204
Fragments arranged by size:
573 336 225 204
SmlI C'TyrA_G
Cuts at: 0 370 1338
Size: 370 968
StuI AGG'CCT
Cuts at: 0 706 958 1145 1338
Size: 706 252 187 193
Fragments arranged by size:
706 252 193 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 1327 1338
Size: 913 414 11
Fragments arranged by size:
913 414 11
TatI w'GTAC_w
Cuts at: 0 173 1338
Size: 173 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 314 346 1338
Size: 314 32 992
Fragments arranged by size:
992 314 32
XmnI GAAnn'nnTTC
Cuts at: 0 259 538 706 790 874 958 1042 1210
Size: 259 279 168 84 84 84 84 168
Cuts at: 1210 1338
Size: 128
Fragments arranged by size:
279 259 168 168 128 84 84 84
84
Enzymes that do cut:
AcuI AleI AlwNI ApoI BamHI BanII BbsI BclI
BmrI BpmI BpuEI BsaI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspMI BsrDI BstEII BstYI Bsu36I
BtgI CspCI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI Hin4I MmeI MslI MspA1I PasI PflMI PpuMI
PvuII SacI SapI SfcI SmlI StuI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BsgI BsiWI
BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EciI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI