Restriction map for
pF1KB8191
(Linear) (Six-base) MAPSORT of: KIBB8191.seq Check: 7684 from: 1 to: 1275
KIBB8191.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 216 1275
Size: 216 1059
AccI GT'mk_AC
Cuts at: 0 1088 1275
Size: 1088 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1275
Size: 1039 236
AfeI AGC'GCT
Cuts at: 0 802 933 967 1275
Size: 802 131 34 308
Fragments arranged by size:
802 308 131 34
ApaLI G'TGCA_C
Cuts at: 0 1107 1275
Size: 1107 168
AvrII C'CTAG_G
Cuts at: 0 810 1275
Size: 810 465
BanI G'GyrC_C
Cuts at: 0 417 977 1275
Size: 417 560 298
Fragments arranged by size:
560 417 298
BanII G_rGCy'C
Cuts at: 0 248 515 1246 1275
Size: 248 267 731 29
Fragments arranged by size:
731 267 248 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 136 900 1275
Size: 136 764 375
Fragments arranged by size:
764 375 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1107 1141 1275
Size: 1107 34 134
Fragments arranged by size:
1107 134 34
BglI GCCn_nnn'nGGC
Cuts at: 0 47 1054 1275
Size: 47 1007 221
Fragments arranged by size:
1007 221 47
BglII A'GATC_T
Cuts at: 0 1022 1275
Size: 1022 253
Bme1580I G_kGCm'C
Cuts at: 0 1111 1275
Size: 1111 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 1053 1275
Size: 1053 222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1275
Size: 172 1103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1275
Size: 109 1166
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 1275
Size: 526 749
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 890 920 1275
Size: 73 30 787 30 355
Fragments arranged by size:
787 355 73 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1275
Size: 94 1181
BseYI C'CCAG_C
Cuts at: 0 45 639 1136 1275
Size: 45 594 497 139
Fragments arranged by size:
594 497 139 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1177 1275
Size: 274 903 98
Fragments arranged by size:
903 274 98
BsiEI CG_ry'CG
Cuts at: 0 32 443 1275
Size: 32 411 832
Fragments arranged by size:
832 411 32
BsiHKAI G_wGCw'C
Cuts at: 0 700 1111 1275
Size: 700 411 164
Fragments arranged by size:
700 411 164
BsmI GAATG_Cn'
Cuts at: 0 843 1275
Size: 843 432
Bsp1286I G_dGCh'C
Cuts at: 0 248 515 700 1111 1246 1275
Size: 248 267 185 411 135 29
Fragments arranged by size:
411 267 248 185 135 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 216 296 595 668 1275
Size: 216 80 299 73 607
Fragments arranged by size:
607 299 216 80 73
BsrBI CCG'CTC
Cuts at: 0 1144 1275
Size: 1144 131
BsrFI r'CCGG_y
Cuts at: 0 218 684 1275
Size: 218 466 591
Fragments arranged by size:
591 466 218
BssSI C'ACGA_G
Cuts at: 0 301 1275
Size: 301 974
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 768 970 1275
Size: 210 558 202 305
Fragments arranged by size:
558 305 210 202
BstYI r'GATC_y
Cuts at: 0 434 998 1022 1275
Size: 434 564 24 253
Fragments arranged by size:
564 434 253 24
BtgI C'CryG_G
Cuts at: 0 314 1275
Size: 314 961
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 291 1275
Size: 291 984
BtsI GCAGTG_nn'
Cuts at: 0 851 1219 1275
Size: 851 368 56
Fragments arranged by size:
851 368 56
DraIII CAC_nnn'GTG
Cuts at: 0 188 1275
Size: 188 1087
EaeI y'GGCC_r
Cuts at: 0 216 280 440 983 1275
Size: 216 64 160 543 292
Fragments arranged by size:
543 292 216 160 64
EagI C'GGCC_G
Cuts at: 0 440 1275
Size: 440 835
EarI CTCTTCn'nnn_
Cuts at: 0 867 1275
Size: 867 408
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1039 1275
Size: 172 867 236
Fragments arranged by size:
867 236 172
EcoO109I rG'GnC_Cy
Cuts at: 0 206 1227 1275
Size: 206 1021 48
Fragments arranged by size:
1021 206 48
HaeII r_GCGC'y
Cuts at: 0 42 804 935 969 1275
Size: 42 762 131 34 306
Fragments arranged by size:
762 306 131 42 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 890 922 1275
Size: 890 32 353
Fragments arranged by size:
890 353 32
HincII GTy'rAC
Cuts at: 0 180 831 1068 1097 1275
Size: 180 651 237 29 178
Fragments arranged by size:
651 237 180 178 29
MscI TGG'CCA
Cuts at: 0 282 1275
Size: 282 993
MslI CAynn'nnrTG
Cuts at: 0 766 1008 1117 1199 1237 1275
Size: 766 242 109 82 38 38
Fragments arranged by size:
766 242 109 82 38 38
MspA1I CmG'CkG
Cuts at: 0 141 1259 1275
Size: 141 1118 16
Fragments arranged by size:
1118 141 16
NaeI GCC'GGC
Cuts at: 0 686 1275
Size: 686 589
NcoI C'CATG_G
Cuts at: 0 314 1275
Size: 314 961
NgoMIV G'CCGG_C
Cuts at: 0 684 1275
Size: 684 591
PflMI CCAn_nnn'nTGG
Cuts at: 0 544 597 1275
Size: 544 53 678
Fragments arranged by size:
678 544 53
PpuMI rG'GwC_Cy
Cuts at: 0 206 1227 1275
Size: 206 1021 48
Fragments arranged by size:
1021 206 48
PstI C_TGCA'G
Cuts at: 0 665 1275
Size: 665 610
PvuI CG_AT'CG
Cuts at: 0 32 1275
Size: 32 1243
PvuII CAG'CTG
Cuts at: 0 141 1275
Size: 141 1134
SfcI C'TryA_G
Cuts at: 0 465 661 1275
Size: 465 196 614
Fragments arranged by size:
614 465 196
SmlI C'TyrA_G
Cuts at: 0 124 1275
Size: 124 1151
SspI AAT'ATT
Cuts at: 0 525 1275
Size: 525 750
StyI C'CwwG_G
Cuts at: 0 314 810 1275
Size: 314 496 465
Fragments arranged by size:
496 465 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1053 1249 1275
Size: 1053 196 26
Fragments arranged by size:
1053 196 26
TatI w'GTAC_w
Cuts at: 0 1148 1275
Size: 1148 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 863 895 1275
Size: 73 32 758 32 380
Fragments arranged by size:
758 380 73 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 355 1225 1275
Size: 355 870 50
Fragments arranged by size:
870 355 50
XmnI GAAnn'nnTTC
Cuts at: 0 1024 1275
Size: 1024 251
Enzymes that do cut:
AarI AccI AcuI AfeI ApaLI AvrII BanI BanII
BbsI BcgI BglI BglII Bme1580I BmrI BpmI BpuEI
BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EagI EarI Eco57MI EcoO109I
HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI
NgoMIV PflMI PpuMI PstI PvuI PvuII SfcI SmlI
SspI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
XbaI XcmI XhoI XmaI ZraI