Restriction map for
pF1KB8198
(Linear) (Six-base) MAPSORT of: KIBB8198.seq Check: 314 from: 1 to: 603
KIBB8198.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 269 603
Size: 269 334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 603
Size: 160 443
AflIII A'CryG_T
Cuts at: 0 92 603
Size: 92 511
AlwNI CAG_nnn'CTG
Cuts at: 0 306 535 603
Size: 306 229 68
Fragments arranged by size:
306 229 68
BanII G_rGCy'C
Cuts at: 0 243 603
Size: 243 360
BglII A'GATC_T
Cuts at: 0 179 603
Size: 179 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 603
Size: 545 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 603
Size: 541 62
BsaAI yAC'GTr
Cuts at: 0 95 603
Size: 95 508
BsaHI Gr'CG_yC
Cuts at: 0 266 603
Size: 266 337
BsaWI w'CCGG_w
Cuts at: 0 209 603
Size: 209 394
BsmI GAATG_Cn'
Cuts at: 0 465 603
Size: 465 138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 439 603
Size: 439 164
Bsp1286I G_dGCh'C
Cuts at: 0 243 603
Size: 243 360
BspEI T'CCGG_A
Cuts at: 0 209 603
Size: 209 394
BspHI T'CATG_A
Cuts at: 0 485 603
Size: 485 118
BsrBI CCG'CTC
Cuts at: 0 546 603
Size: 546 57
BsrFI r'CCGG_y
Cuts at: 0 582 603
Size: 582 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 65 306 603
Size: 65 241 297
Fragments arranged by size:
297 241 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 126 153 387 603
Size: 126 27 234 216
Fragments arranged by size:
234 216 126 27
BstYI r'GATC_y
Cuts at: 0 137 179 603
Size: 137 42 424
Fragments arranged by size:
424 137 42
BtgI C'CryG_G
Cuts at: 0 491 603
Size: 491 112
BtsI GCAGTG_nn'
Cuts at: 0 307 603
Size: 307 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 545 603
Size: 160 385 58
Fragments arranged by size:
385 160 58
EcoO109I rG'GnC_Cy
Cuts at: 0 520 603
Size: 520 83
HaeII r_GCGC'y
Cuts at: 0 455 603
Size: 455 148
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 603
Size: 265 32 306
Fragments arranged by size:
306 265 32
MslI CAynn'nnrTG
Cuts at: 0 124 253 256 490 603
Size: 124 129 3 234 113
Fragments arranged by size:
234 129 124 113 3
MspA1I CmG'CkG
Cuts at: 0 193 603
Size: 193 410
NaeI GCC'GGC
Cuts at: 0 584 603
Size: 584 19
NcoI C'CATG_G
Cuts at: 0 491 603
Size: 491 112
NgoMIV G'CCGG_C
Cuts at: 0 582 603
Size: 582 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 603
Size: 466 32 105
Fragments arranged by size:
466 105 32
PstI C_TGCA'G
Cuts at: 0 311 603
Size: 311 292
SfcI C'TryA_G
Cuts at: 0 307 603
Size: 307 296
SmlI C'TyrA_G
Cuts at: 0 556 603
Size: 556 47
StyI C'CwwG_G
Cuts at: 0 407 491 603
Size: 407 84 112
Fragments arranged by size:
407 112 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 387 603
Size: 384 3 216
Fragments arranged by size:
384 216 3
ZraI GAC'GTC
Cuts at: 0 267 603
Size: 267 336
Enzymes that do cut:
AatII AcuI AflIII AlwNI BanII BglII BpmI BpuEI
BsaAI BsaHI BsaWI BsmI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrFI BstAPI BstXI BstYI BtgI BtsI Eco57MI
EcoO109I HaeII Hin4I MslI MspA1I NaeI NcoI NgoMIV
PpiI PstI SfcI SmlI StyI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BspMI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI