(Linear) (Six-base) MAPSORT of: KIBB8201.seq  Check: 725  from: 1  to: 609



KIBB8201.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0     260     609 
   Size:        260     349
 
AvaI C'yCGr_G

Cuts at:      0      48     419     497     609 
   Size:         48     371      78     112

  Fragments arranged by size: 

                 371     112      78      48
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     433     467     609 
   Size:        433      34     142

  Fragments arranged by size: 

                 433     142      34
 
BclI T'GATC_A

Cuts at:      0      71     609 
   Size:         71     538
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     548     609 
   Size:        548      61
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     345     609 
   Size:        345     264
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     609 
   Size:        496     113
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     583     609 
   Size:        583      26
 
BseYI C'CCAG_C

Cuts at:      0     334     354     609 
   Size:        334      20     255

  Fragments arranged by size: 

                 334     255      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     609 
   Size:        412     197
 
BsiHKAI G_wGCw'C

Cuts at:      0     601     609 
   Size:        601       8
 
BsmI GAATG_Cn'

Cuts at:      0     330     609 
   Size:        330     279
 
Bsp1286I G_dGCh'C

Cuts at:      0     601     609 
   Size:        601       8
 
BsrBI CCG'CTC

Cuts at:      0     204     609 
   Size:        204     405
 
BstBI TT'CG_AA

Cuts at:      0     443     609 
   Size:        443     166
 
BstYI r'GATC_y

Cuts at:      0     137     609 
   Size:        137     472
 
BstZ17I GTA'TAC

Cuts at:      0     261     609 
   Size:        261     348
 
BtgI C'CryG_G

Cuts at:      0     234     242     609 
   Size:        234       8     367

  Fragments arranged by size: 

                 367     234       8
 
EaeI y'GGCC_r

Cuts at:      0       2     195     609 
   Size:          2     193     414

  Fragments arranged by size: 

                 414     193       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      28     609 
   Size:         28     581
 
HaeII r_GCGC'y

Cuts at:      0     348     609 
   Size:        348     261
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     609 
   Size:        265      32     312

  Fragments arranged by size: 

                 312     265      32
 
MscI TGG'CCA

Cuts at:      0       4     197     609 
   Size:          4     193     412

  Fragments arranged by size: 

                 412     193       4
 
NcoI C'CATG_G

Cuts at:      0     242     609 
   Size:        242     367
 
PasI CC'CwG_GG

Cuts at:      0     603     609 
   Size:        603       6
 
ScaI AGT'ACT

Cuts at:      0     558     609 
   Size:        558      51
 
SmaI CCC'GGG

Cuts at:      0     499     609 
   Size:        499     110
 
SmlI C'TyrA_G

Cuts at:      0     419     511     609 
   Size:        419      92      98

  Fragments arranged by size: 

                 419      98      92
 
SpeI A'CTAG_T

Cuts at:      0     448     609 
   Size:        448     161
 
SspI AAT'ATT

Cuts at:      0     294     609 
   Size:        294     315
 
StyI C'CwwG_G

Cuts at:      0     242     609 
   Size:        242     367
 
TatI w'GTAC_w

Cuts at:      0     556     609 
   Size:        556      53
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     351     383     609 
   Size:        351      32     226

  Fragments arranged by size: 

                 351     226      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     468     609 
   Size:        468     141
 
XhoI C'TCGA_G

Cuts at:      0     419     609 
   Size:        419     190
 
XmaI C'CCGG_G

Cuts at:      0     497     609 
   Size:        497     112
 
XmnI GAAnn'nnTTC

Cuts at:      0     283     609 
   Size:        283     326
 

 Enzymes that do cut:

     AccI     AvaI     BcgI     BclI     BmrI     BplI    BpuEI    BsaXI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI    BstBI    BstYI
  BstZ17I     BtgI     EaeI     EarI    HaeII    Hin4I     MscI     NcoI
     PasI     ScaI     SmaI     SmlI     SpeI     SspI     StyI     TatI
     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SphI
     SrfI     StuI     SwaI    TaqII  Tth111I     XbaI     ZraI