(Linear) (Six-base) MAPSORT of: KIBB8202.seq  Check: 435  from: 1  to: 582



KIBB8202.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     178     582 
   Size:        178     404
 
ApoI r'AATT_y

Cuts at:      0       6     283     582 
   Size:          6     277     299

  Fragments arranged by size: 

                 299     277       6
 
BamHI G'GATC_C

Cuts at:      0     555     582 
   Size:        555      27
 
BanII G_rGCy'C

Cuts at:      0      30     582 
   Size:         30     552
 
BclI T'GATC_A

Cuts at:      0     248     582 
   Size:        248     334
 
BfrBI ATG'CAT

Cuts at:      0     498     582 
   Size:        498      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     572     582 
   Size:        572      10
 
Bpu10I CC'TnA_GC

Cuts at:      0      29     582 
   Size:         29     553
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     138     168     582 
   Size:        138      30     414

  Fragments arranged by size: 

                 414     138      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     143     582 
   Size:        143     439
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     582 
   Size:         30     552
 
BspHI T'CATG_A

Cuts at:      0     200     582 
   Size:        200     382
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     341     582 
   Size:        341     241
 
BsrBI CCG'CTC

Cuts at:      0     383     582 
   Size:        383     199
 
BsrFI r'CCGG_y

Cuts at:      0     448     582 
   Size:        448     134
 
BstYI r'GATC_y

Cuts at:      0     555     582 
   Size:        555      27
 
BtgI C'CryG_G

Cuts at:      0     140     146     582 
   Size:        140       6     436

  Fragments arranged by size: 

                 436     140       6
 
BtsI GCAGTG_nn'

Cuts at:      0     490     582 
   Size:        490      92
 
EaeI y'GGCC_r

Cuts at:      0     143     446     582 
   Size:        143     303     136

  Fragments arranged by size: 

                 303     143     136
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     445     582 
   Size:        445     137
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     431     582 
   Size:        431     151
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     582 
   Size:        115     467
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     333     365     582 
   Size:        333      32     217

  Fragments arranged by size: 

                 333     217      32
 
MspA1I CmG'CkG

Cuts at:      0     142     582 
   Size:        142     440
 
NaeI GCC'GGC

Cuts at:      0     450     582 
   Size:        450     132
 
NgoMIV G'CCGG_C

Cuts at:      0     448     582 
   Size:        448     134
 
NsiI A_TGCA'T

Cuts at:      0     500     582 
   Size:        500      82
 
PpuMI rG'GwC_Cy

Cuts at:      0     115     582 
   Size:        115     467
 
SacII CC_GC'GG

Cuts at:      0     143     582 
   Size:        143     439
 
SanDI GG'GwC_CC

Cuts at:      0     115     582 
   Size:        115     467
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     138     170     582 
   Size:        138      32     412

  Fragments arranged by size: 

                 412     138      32
 

 Enzymes that do cut:

    AlwNI     ApoI    BamHI    BanII     BclI    BfrBI     BmrI   Bpu10I
    BsaXI    BseRI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BstYI
     BtgI     BtsI     EaeI     EciI    EcoNI EcoO109I    Hin4I   MspA1I
     NaeI   NgoMIV     NsiI    PpuMI    SacII    SanDI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI