Restriction map for
pF1KB8204
(Linear) (Six-base) MAPSORT of: KIBB8204.seq Check: 7985 from: 1 to: 1410
KIBB8204.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 940 992 1304 1410
Size: 421 519 52 312 106
Fragments arranged by size:
519 421 312 106 52
AhdI GACnn_n'nnGTC
Cuts at: 0 8 1410
Size: 8 1402
AlwNI CAG_nnn'CTG
Cuts at: 0 819 1380 1392 1410
Size: 819 561 12 18
Fragments arranged by size:
819 561 18 12
ApaI G_GGCC'C
Cuts at: 0 94 1410
Size: 94 1316
AvaI C'yCGr_G
Cuts at: 0 43 96 187 310 1410
Size: 43 53 91 123 1100
Fragments arranged by size:
1100 123 91 53 43
BamHI G'GATC_C
Cuts at: 0 756 1410
Size: 756 654
BanI G'GyrC_C
Cuts at: 0 81 222 1410
Size: 81 141 1188
Fragments arranged by size:
1188 141 81
BanII G_rGCy'C
Cuts at: 0 63 94 103 161 1410
Size: 63 31 9 58 1249
Fragments arranged by size:
1249 63 58 31 9
BbeI G_GCGC'C
Cuts at: 0 85 1410
Size: 85 1325
BbsI GAAGACnn'nnnn_
Cuts at: 0 4 841 1027 1410
Size: 4 837 186 383
Fragments arranged by size:
837 383 186 4
BbvCI CC'TCA_GC
Cuts at: 0 476 1410
Size: 476 934
BglI GCCn_nnn'nGGC
Cuts at: 0 98 1410
Size: 98 1312
BglII A'GATC_T
Cuts at: 0 603 1410
Size: 603 807
BlpI GC'TnA_GC
Cuts at: 0 801 1189 1410
Size: 801 388 221
Fragments arranged by size:
801 388 221
Bme1580I G_kGCm'C
Cuts at: 0 94 227 1410
Size: 94 133 1183
Fragments arranged by size:
1183 133 94
BmgBI CAC'GTC
Cuts at: 0 1044 1410
Size: 1044 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 738 1410
Size: 738 672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 1182 1402 1410
Size: 77 1105 220 8
Fragments arranged by size:
1105 220 77 8
Bpu10I CC'TnA_GC
Cuts at: 0 476 1410
Size: 476 934
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1237 1410
Size: 1150 87 173
Fragments arranged by size:
1150 173 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 1196 1410
Size: 1196 214
BsaHI Gr'CG_yC
Cuts at: 0 82 1410
Size: 82 1328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 234 264 1410
Size: 234 30 1146
Fragments arranged by size:
1146 234 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 273 709 1069 1303 1410
Size: 273 436 360 234 107
Fragments arranged by size:
436 360 273 234 107
BseYI C'CCAG_C
Cuts at: 0 541 777 938 1410
Size: 541 236 161 472
Fragments arranged by size:
541 472 236 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 769 1410
Size: 693 76 641
Fragments arranged by size:
693 641 76
BsiHKAI G_wGCw'C
Cuts at: 0 1122 1410
Size: 1122 288
BsmBI CGTCTCn'nnnn_
Cuts at: 0 655 1135 1410
Size: 655 480 275
Fragments arranged by size:
655 480 275
Bsp1286I G_dGCh'C
Cuts at: 0 63 94 103 161 227 1122 1410
Size: 63 31 9 58 66 895 288
Fragments arranged by size:
895 288 66 63 58 31 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1235 1410
Size: 1235 175
BsrBI CCG'CTC
Cuts at: 0 800 1235 1410
Size: 800 435 175
Fragments arranged by size:
800 435 175
BsrDI GCAATG_nn'
Cuts at: 0 494 1410
Size: 494 916
BsrFI r'CCGG_y
Cuts at: 0 219 1273 1410
Size: 219 1054 137
Fragments arranged by size:
1054 219 137
BstYI r'GATC_y
Cuts at: 0 603 756 1133 1169 1358 1410
Size: 603 153 377 36 189 52
Fragments arranged by size:
603 377 189 153 52 36
BtgI C'CryG_G
Cuts at: 0 29 86 146 1094 1410
Size: 29 57 60 948 316
Fragments arranged by size:
948 316 60 57 29
BtsI GCAGTG_nn'
Cuts at: 0 255 1410
Size: 255 1155
DrdI GACnn_nn'nnGTC
Cuts at: 0 976 1149 1410
Size: 976 173 261
Fragments arranged by size:
976 261 173
EaeI y'GGCC_r
Cuts at: 0 1097 1410
Size: 1097 313
EarI CTCTTCn'nnn_
Cuts at: 0 397 570 1280 1410
Size: 397 173 710 130
Fragments arranged by size:
710 397 173 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 421 940 992 1182 1304 1402 1410
Size: 77 344 519 52 190 122 98 8
Fragments arranged by size:
519 344 190 122 98 77 52 8
EcoO109I rG'GnC_Cy
Cuts at: 0 91 693 816 1248 1410
Size: 91 602 123 432 162
Fragments arranged by size:
602 432 162 123 91
FspI TGC'GCA
Cuts at: 0 1402 1410
Size: 1402 8
HaeII r_GCGC'y
Cuts at: 0 42 85 181 945 1296 1410
Size: 42 43 96 764 351 114
Fragments arranged by size:
764 351 114 96 43 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 425 457 1104 1136 1410
Size: 26 399 32 647 32 274
Fragments arranged by size:
647 399 274 32 32 26
HindIII A'AGCT_T
Cuts at: 0 1191 1410
Size: 1191 219
KasI G'GCGC_C
Cuts at: 0 81 1410
Size: 81 1329
MfeI C'AATT_G
Cuts at: 0 974 1410
Size: 974 436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 509 1410
Size: 470 39 901
Fragments arranged by size:
901 470 39
MslI CAynn'nnrTG
Cuts at: 0 997 1042 1410
Size: 997 45 368
Fragments arranged by size:
997 368 45
MspA1I CmG'CkG
Cuts at: 0 148 1410
Size: 148 1262
NaeI GCC'GGC
Cuts at: 0 1275 1410
Size: 1275 135
NarI GG'CG_CC
Cuts at: 0 82 1410
Size: 82 1328
NcoI C'CATG_G
Cuts at: 0 86 1410
Size: 86 1324
NgoMIV G'CCGG_C
Cuts at: 0 1273 1410
Size: 1273 137
PfoI T'CCnGG_A
Cuts at: 0 389 530 1410
Size: 389 141 880
Fragments arranged by size:
880 389 141
PpuMI rG'GwC_Cy
Cuts at: 0 693 1248 1410
Size: 693 555 162
Fragments arranged by size:
693 555 162
PspOMI G'GGCC_C
Cuts at: 0 90 1410
Size: 90 1320
PstI C_TGCA'G
Cuts at: 0 851 1410
Size: 851 559
SacII CC_GC'GG
Cuts at: 0 149 1410
Size: 149 1261
SfcI C'TryA_G
Cuts at: 0 847 1410
Size: 847 563
SfoI GGC'GCC
Cuts at: 0 83 1410
Size: 83 1327
SmaI CCC'GGG
Cuts at: 0 45 98 189 1410
Size: 45 53 91 1221
Fragments arranged by size:
1221 91 53 45
SmlI C'TyrA_G
Cuts at: 0 1165 1252 1410
Size: 1165 87 158
Fragments arranged by size:
1165 158 87
SrfI GCCC'GGGC
Cuts at: 0 189 1410
Size: 189 1221
StyI C'CwwG_G
Cuts at: 0 86 1410
Size: 86 1324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 35 39 663 1410
Size: 35 4 624 747
Fragments arranged by size:
747 624 35 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 234 266 1410
Size: 234 32 1144
Fragments arranged by size:
1144 234 32
XmaI C'CCGG_G
Cuts at: 0 43 96 187 1410
Size: 43 53 91 1223
Fragments arranged by size:
1223 91 53 43
Enzymes that do cut:
AcuI AhdI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI
EcoO109I FspI HaeII Hin4I HindIII KasI MfeI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV PfoI PpuMI
PspOMI PstI SacII SfcI SfoI SmaI SmlI SrfI
StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BmtI
BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
HincII HpaI KpnI MluI MscI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI
SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI