Restriction map for
pF1KB8206
(Linear) (Six-base) MAPSORT of: KIBB8206.seq Check: 8019 from: 1 to: 807
KIBB8206.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AgeI A'CCGG_T
Cuts at: 0 328 807
Size: 328 479
AleI CACnn'nnGTG
Cuts at: 0 539 807
Size: 539 268
AlwNI CAG_nnn'CTG
Cuts at: 0 754 807
Size: 754 53
ApaLI G'TGCA_C
Cuts at: 0 737 807
Size: 737 70
AvaI C'yCGr_G
Cuts at: 0 5 568 807
Size: 5 563 239
Fragments arranged by size:
563 239 5
BanI G'GyrC_C
Cuts at: 0 54 807
Size: 54 753
BanII G_rGCy'C
Cuts at: 0 167 569 647 807
Size: 167 402 78 160
Fragments arranged by size:
402 167 160 78
BbeI G_GCGC'C
Cuts at: 0 58 807
Size: 58 749
BbsI GAAGACnn'nnnn_
Cuts at: 0 776 807
Size: 776 31
BbvCI CC'TCA_GC
Cuts at: 0 355 807
Size: 355 452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 62 96 807
Size: 62 34 711
Fragments arranged by size:
711 62 34
BglI GCCn_nnn'nGGC
Cuts at: 0 43 807
Size: 43 764
BglII A'GATC_T
Cuts at: 0 714 764 807
Size: 714 50 43
Fragments arranged by size:
714 50 43
Bme1580I G_kGCm'C
Cuts at: 0 48 85 546 741 807
Size: 48 37 461 195 66
Fragments arranged by size:
461 195 66 48 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 259 744 807
Size: 259 485 63
Fragments arranged by size:
485 259 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 807
Size: 739 68
Bpu10I CC'TnA_GC
Cuts at: 0 355 807
Size: 355 452
BsaHI Gr'CG_yC
Cuts at: 0 55 807
Size: 55 752
BsaWI w'CCGG_w
Cuts at: 0 328 634 664 807
Size: 328 306 30 143
Fragments arranged by size:
328 306 143 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 468 807
Size: 46 422 339
Fragments arranged by size:
422 339 46
BseYI C'CCAG_C
Cuts at: 0 372 807
Size: 372 435
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 807
Size: 132 675
BsiHKAI G_wGCw'C
Cuts at: 0 167 647 741 807
Size: 167 480 94 66
Fragments arranged by size:
480 167 94 66
Bsp1286I G_dGCh'C
Cuts at: 0 48 85 167 546 569 647 741 807
Size: 48 37 82 379 23 78 94 66
Fragments arranged by size:
379 94 82 78 66 48 37 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 475 807
Size: 475 332
BsrFI r'CCGG_y
Cuts at: 0 328 807
Size: 328 479
BstEII G'GTnAC_C
Cuts at: 0 579 807
Size: 579 228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 313 807
Size: 313 494
BstYI r'GATC_y
Cuts at: 0 714 764 807
Size: 714 50 43
Fragments arranged by size:
714 50 43
BtgI C'CryG_G
Cuts at: 0 306 773 807
Size: 306 467 34
Fragments arranged by size:
467 306 34
BtsI GCAGTG_nn'
Cuts at: 0 123 334 807
Size: 123 211 473
Fragments arranged by size:
473 211 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 214 807
Size: 214 593
EcoICRI GAG'CTC
Cuts at: 0 165 645 807
Size: 165 480 162
Fragments arranged by size:
480 165 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 807
Size: 739 68
HaeII r_GCGC'y
Cuts at: 0 58 807
Size: 58 749
KasI G'GCGC_C
Cuts at: 0 54 807
Size: 54 753
MslI CAynn'nnrTG
Cuts at: 0 539 807
Size: 539 268
MspA1I CmG'CkG
Cuts at: 0 17 264 488 807
Size: 17 247 224 319
Fragments arranged by size:
319 247 224 17
NarI GG'CG_CC
Cuts at: 0 55 807
Size: 55 752
NcoI C'CATG_G
Cuts at: 0 306 773 807
Size: 306 467 34
Fragments arranged by size:
467 306 34
NspI r_CATG'y
Cuts at: 0 305 807
Size: 305 502
PvuII CAG'CTG
Cuts at: 0 488 807
Size: 488 319
SacI G_AGCT'C
Cuts at: 0 167 647 807
Size: 167 480 160
Fragments arranged by size:
480 167 160
SfoI GGC'GCC
Cuts at: 0 56 807
Size: 56 751
SmaI CCC'GGG
Cuts at: 0 570 807
Size: 570 237
SphI G_CATG'C
Cuts at: 0 305 807
Size: 305 502
StuI AGG'CCT
Cuts at: 0 37 807
Size: 37 770
StyI C'CwwG_G
Cuts at: 0 306 773 807
Size: 306 467 34
Fragments arranged by size:
467 306 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 807
Size: 500 32 275
Fragments arranged by size:
500 275 32
XmaI C'CCGG_G
Cuts at: 0 568 807
Size: 568 239
Enzymes that do cut:
AgeI AleI AlwNI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII Bme1580I BplI BpmI
Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrFI BstEII BstXI BstYI BtgI BtsI EciI
EcoICRI Eco57MI HaeII KasI MslI MspA1I NarI NcoI
NspI PvuII SacI SfoI SmaI SphI StuI StyI
TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmnI ZraI