(Linear) (Six-base) MAPSORT of: KIBB8208.seq  Check: 4629  from: 1  to: 375



KIBB8208.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

ApaLI G'TGCA_C

Cuts at:      0     122     375 
   Size:        122     253
 
ApoI r'AATT_y

Cuts at:      0     151     375 
   Size:        151     224
 
AvaI C'yCGr_G

Cuts at:      0      65     128     375 
   Size:         65      63     247

  Fragments arranged by size: 

                 247      65      63
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     308     341     375 
   Size:        308      33      34

  Fragments arranged by size: 

                 308      34      33
 
Bme1580I G_kGCm'C

Cuts at:      0     126     135     375 
   Size:        126       9     240

  Fragments arranged by size: 

                 240     126       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     375 
   Size:        161     214
 
BsiHKAI G_wGCw'C

Cuts at:      0     126     375 
   Size:        126     249
 
Bsp1286I G_dGCh'C

Cuts at:      0     126     135     375 
   Size:        126       9     240

  Fragments arranged by size: 

                 240     126       9
 
BstEII G'GTnAC_C

Cuts at:      0      60     315     375 
   Size:         60     255      60

  Fragments arranged by size: 

                 255      60      60
 
BtgI C'CryG_G

Cuts at:      0      99     375 
   Size:         99     276
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     375 
   Size:        161     214
 
FspI TGC'GCA

Cuts at:      0     187     375 
   Size:        187     188
 
FspAI rTGC'GCAy

Cuts at:      0     187     375 
   Size:        187     188
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     375 
   Size:        159      32     184

  Fragments arranged by size: 

                 184     159      32
 
NcoI C'CATG_G

Cuts at:      0      99     375 
   Size:         99     276
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     105     375 
   Size:        105     270
 
SfcI C'TryA_G

Cuts at:      0     349     375 
   Size:        349      26
 
StyI C'CwwG_G

Cuts at:      0      99     375 
   Size:         99     276
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      54     165     375 
   Size:         54     111     210

  Fragments arranged by size: 

                 210     111      54
 

 Enzymes that do cut:

    ApaLI     ApoI     AvaI     BaeI Bme1580I     BpmI  BsiHKAI Bsp1286I
   BstEII     BtgI  Eco57MI     FspI    FspAI    Hin4I     NcoI    PflMI
     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI