Restriction map for
pF1KB8214
(Linear) (Six-base) MAPSORT of: KIBB8214.seq Check: 4359 from: 1 to: 771
KIBB8214.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 594 771
Size: 594 177
AgeI A'CCGG_T
Cuts at: 0 710 771
Size: 710 61
AlwNI CAG_nnn'CTG
Cuts at: 0 513 654 771
Size: 513 141 117
Fragments arranged by size:
513 141 117
ApaI G_GGCC'C
Cuts at: 0 245 771
Size: 245 526
AvaI C'yCGr_G
Cuts at: 0 244 259 771
Size: 244 15 512
Fragments arranged by size:
512 244 15
BamHI G'GATC_C
Cuts at: 0 482 771
Size: 482 289
BanI G'GyrC_C
Cuts at: 0 61 310 346 556 771
Size: 61 249 36 210 215
Fragments arranged by size:
249 215 210 61 36
BanII G_rGCy'C
Cuts at: 0 97 245 251 697 771
Size: 97 148 6 446 74
Fragments arranged by size:
446 148 97 74 6
BbeI G_GCGC'C
Cuts at: 0 314 350 560 771
Size: 314 36 210 211
Fragments arranged by size:
314 211 210 36
BglI GCCn_nnn'nGGC
Cuts at: 0 178 771
Size: 178 593
Bme1580I G_kGCm'C
Cuts at: 0 76 245 434 771
Size: 76 169 189 337
Fragments arranged by size:
337 189 169 76
BmgBI CAC'GTC
Cuts at: 0 429 771
Size: 429 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 676 771
Size: 541 135 95
Fragments arranged by size:
541 135 95
BsaAI yAC'GTr
Cuts at: 0 549 771
Size: 549 222
BsaHI Gr'CG_yC
Cuts at: 0 311 347 557 771
Size: 311 36 210 214
Fragments arranged by size:
311 214 210 36
BsaWI w'CCGG_w
Cuts at: 0 626 710 771
Size: 626 84 61
Fragments arranged by size:
626 84 61
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 771
Size: 673 98
BseYI C'CCAG_C
Cuts at: 0 163 306 350 771
Size: 163 143 44 421
Fragments arranged by size:
421 163 143 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 771
Size: 436 335
BsiEI CG_ry'CG
Cuts at: 0 116 173 209 771
Size: 116 57 36 562
Fragments arranged by size:
562 116 57 36
BsiHKAI G_wGCw'C
Cuts at: 0 251 771
Size: 251 520
BsmBI CGTCTCn'nnnn_
Cuts at: 0 592 771
Size: 592 179
Bsp1286I G_dGCh'C
Cuts at: 0 76 97 245 251 434 697 771
Size: 76 21 148 6 183 263 74
Fragments arranged by size:
263 183 148 76 74 21 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 594 771
Size: 594 177
BsrBI CCG'CTC
Cuts at: 0 132 227 564 771
Size: 132 95 337 207
Fragments arranged by size:
337 207 132 95
BsrDI GCAATG_nn'
Cuts at: 0 719 771
Size: 719 52
BsrFI r'CCGG_y
Cuts at: 0 64 577 710 771
Size: 64 513 133 61
Fragments arranged by size:
513 133 64 61
BssHII G'CGCG_C
Cuts at: 0 525 771
Size: 525 246
BssSI C'ACGA_G
Cuts at: 0 250 771
Size: 250 521
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 771
Size: 513 258
BstYI r'GATC_y
Cuts at: 0 482 771
Size: 482 289
BtgI C'CryG_G
Cuts at: 0 11 140 203 212 771
Size: 11 129 63 9 559
Fragments arranged by size:
559 129 63 11 9
ClaI AT'CG_AT
Cuts at: 0 752 771
Size: 752 19
DraIII CAC_nnn'GTG
Cuts at: 0 549 771
Size: 549 222
DrdI GACnn_nn'nnGTC
Cuts at: 0 754 771
Size: 754 17
EaeI y'GGCC_r
Cuts at: 0 14 47 113 170 206 405 575 771
Size: 14 33 66 57 36 199 170 196
Fragments arranged by size:
199 196 170 66 57 36 33 14
EagI C'GGCC_G
Cuts at: 0 113 170 206 771
Size: 113 57 36 565
Fragments arranged by size:
565 113 57 36
EcoICRI GAG'CTC
Cuts at: 0 249 771
Size: 249 522
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 676 771
Size: 541 135 95
Fragments arranged by size:
541 135 95
EcoO109I rG'GnC_Cy
Cuts at: 0 360 384 667 771
Size: 360 24 283 104
Fragments arranged by size:
360 283 104 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 42 771
Size: 42 729
HaeII r_GCGC'y
Cuts at: 0 148 314 350 560 771
Size: 148 166 36 210 211
Fragments arranged by size:
211 210 166 148 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 745 771
Size: 276 32 437 26
Fragments arranged by size:
437 276 32 26
KasI G'GCGC_C
Cuts at: 0 310 346 556 771
Size: 310 36 210 215
Fragments arranged by size:
310 215 210 36
MscI TGG'CCA
Cuts at: 0 49 771
Size: 49 722
MspA1I CmG'CkG
Cuts at: 0 13 205 214 306 321 510 573 771
Size: 13 192 9 92 15 189 63 198
Fragments arranged by size:
198 192 189 92 63 15 13 9
NaeI GCC'GGC
Cuts at: 0 579 771
Size: 579 192
NarI GG'CG_CC
Cuts at: 0 311 347 557 771
Size: 311 36 210 214
Fragments arranged by size:
311 214 210 36
NgoMIV G'CCGG_C
Cuts at: 0 577 771
Size: 577 194
NotI GC'GGCC_GC
Cuts at: 0 113 206 771
Size: 113 93 565
Fragments arranged by size:
565 113 93
PasI CC'CwG_GG
Cuts at: 0 380 771
Size: 380 391
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 549 771
Size: 105 444 222
Fragments arranged by size:
444 222 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 771
Size: 238 32 501
Fragments arranged by size:
501 238 32
PpuMI rG'GwC_Cy
Cuts at: 0 360 384 771
Size: 360 24 387
Fragments arranged by size:
387 360 24
PspOMI G'GGCC_C
Cuts at: 0 241 771
Size: 241 530
PstI C_TGCA'G
Cuts at: 0 326 771
Size: 326 445
PvuII CAG'CTG
Cuts at: 0 306 510 573 771
Size: 306 204 63 198
Fragments arranged by size:
306 204 198 63
SacI G_AGCT'C
Cuts at: 0 251 771
Size: 251 520
SacII CC_GC'GG
Cuts at: 0 14 206 215 771
Size: 14 192 9 556
Fragments arranged by size:
556 192 14 9
SanDI GG'GwC_CC
Cuts at: 0 360 771
Size: 360 411
SfcI C'TryA_G
Cuts at: 0 322 771
Size: 322 449
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 178 771
Size: 178 593
SfoI GGC'GCC
Cuts at: 0 312 348 558 771
Size: 312 36 210 213
Fragments arranged by size:
312 213 210 36
SgrAI Cr'CCGG_yG
Cuts at: 0 64 771
Size: 64 707
StyI C'CwwG_G
Cuts at: 0 41 50 771
Size: 41 9 721
Fragments arranged by size:
721 41 9
TatI w'GTAC_w
Cuts at: 0 638 771
Size: 638 133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 771
Size: 39 732
XmnI GAAnn'nnTTC
Cuts at: 0 397 771
Size: 397 374
Enzymes that do cut:
AarI AgeI AlwNI ApaI AvaI BamHI BanI BanII
BbeI BglI Bme1580I BmgBI BpmI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstYI BtgI
ClaI DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I KasI MscI MspA1I NaeI NarI
NgoMIV NotI PasI PflMI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SfcI SfiI SfoI SgrAI
StyI TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaXI BsiWI BsmI BspEI BspHI BsrGI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI
DraI EarI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI NcoI NdeI NheI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI