(Linear) (Six-base) MAPSORT of: KIBB8215.seq  Check: 2957  from: 1  to: 420



KIBB8215.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     361     420 
   Size:        169     192      59

  Fragments arranged by size: 

                 192     169      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     385     420 
   Size:        385      35
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     420 
   Size:        129     291
 
ApaLI G'TGCA_C

Cuts at:      0     415     420 
   Size:        415       5
 
BanII G_rGCy'C

Cuts at:      0     168     420 
   Size:        168     252
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     349     420 
   Size:        349      71
 
Bme1580I G_kGCm'C

Cuts at:      0     419     420 
   Size:        419       1
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     117     420 
   Size:        117     303
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     182     420 
   Size:         47     135     238

  Fragments arranged by size: 

                 238     135      47
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      68     203     420 
   Size:         68     135     217

  Fragments arranged by size: 

                 217     135      68
 
BseYI C'CCAG_C

Cuts at:      0     203     420 
   Size:        203     217
 
BsiHKAI G_wGCw'C

Cuts at:      0     168     320     419     420 
   Size:        168     152      99       1

  Fragments arranged by size: 

                 168     152      99       1
 
Bsp1286I G_dGCh'C

Cuts at:      0     168     320     419     420 
   Size:        168     152      99       1

  Fragments arranged by size: 

                 168     152      99       1
 
BspHI T'CATG_A

Cuts at:      0      81     420 
   Size:         81     339
 
BssSI C'ACGA_G

Cuts at:      0     350     420 
   Size:        350      70
 
BstYI r'GATC_y

Cuts at:      0     335     420 
   Size:        335      85
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     379     420 
   Size:        379      41
 
EcoICRI GAG'CTC

Cuts at:      0     166     420 
   Size:        166     254
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     169     182     361     420 
   Size:         47     122      13     179      59

  Fragments arranged by size: 

                 179     122      59      47      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      63      95     167     199     420 
   Size:         63      32      72      32     221

  Fragments arranged by size: 

                 221      72      63      32      32
 
HincII GTy'rAC

Cuts at:      0     119     420 
   Size:        119     301
 
MslI CAynn'nnrTG

Cuts at:      0     367     420 
   Size:        367      53
 
NspI r_CATG'y

Cuts at:      0     366     420 
   Size:        366      54
 
PfoI T'CCnGG_A

Cuts at:      0     159     420 
   Size:        159     261
 
PstI C_TGCA'G

Cuts at:      0     266     420 
   Size:        266     154
 
SacI G_AGCT'C

Cuts at:      0     168     420 
   Size:        168     252
 
SfcI C'TryA_G

Cuts at:      0     262     420 
   Size:        262     158
 
SphI G_CATG'C

Cuts at:      0     366     420 
   Size:        366      54
 
StuI AGG'CCT

Cuts at:      0     199     420 
   Size:        199     221
 
StyI C'CwwG_G

Cuts at:      0     224     420 
   Size:        224     196
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    ApaLI    BanII     BbsI Bme1580I     BmrI
     BpmI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI    BssSI    BstYI
    BtgZI  EcoICRI  Eco57MI    Hin4I   HincII     MslI     NspI     PfoI
     PstI     SacI     SfcI     SphI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI