Restriction map for
pF1KB8221
(Linear) (Six-base) MAPSORT of: KIBB8221.seq Check: 8402 from: 1 to: 744
KIBB8221.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 612 744
Size: 612 132
AlwNI CAG_nnn'CTG
Cuts at: 0 319 625 744
Size: 319 306 119
Fragments arranged by size:
319 306 119
ApaI G_GGCC'C
Cuts at: 0 185 744
Size: 185 559
ApaLI G'TGCA_C
Cuts at: 0 739 744
Size: 739 5
AvaI C'yCGr_G
Cuts at: 0 178 744
Size: 178 566
BamHI G'GATC_C
Cuts at: 0 12 744
Size: 12 732
BanI G'GyrC_C
Cuts at: 0 22 34 73 172 744
Size: 22 12 39 99 572
Fragments arranged by size:
572 99 39 22 12
BanII G_rGCy'C
Cuts at: 0 185 744
Size: 185 559
BbeI G_GCGC'C
Cuts at: 0 26 38 77 176 744
Size: 26 12 39 99 568
Fragments arranged by size:
568 99 39 26 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 744
Size: 92 652
BciVI GTATCCnnnnn_n'
Cuts at: 0 337 744
Size: 337 407
BglI GCCn_nnn'nGGC
Cuts at: 0 106 744
Size: 106 638
BlpI GC'TnA_GC
Cuts at: 0 5 744
Size: 5 739
Bme1580I G_kGCm'C
Cuts at: 0 185 743 744
Size: 185 558 1
Fragments arranged by size:
558 185 1
BmgBI CAC'GTC
Cuts at: 0 525 744
Size: 525 219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 744
Size: 706 38
BsaHI Gr'CG_yC
Cuts at: 0 23 35 74 173 744
Size: 23 12 39 99 571
Fragments arranged by size:
571 99 39 23 12
BsaWI w'CCGG_w
Cuts at: 0 213 744
Size: 213 531
BseYI C'CCAG_C
Cuts at: 0 54 185 744
Size: 54 131 559
Fragments arranged by size:
559 131 54
BsiHKAI G_wGCw'C
Cuts at: 0 743 744
Size: 743 1
Bsp1286I G_dGCh'C
Cuts at: 0 185 743 744
Size: 185 558 1
Fragments arranged by size:
558 185 1
BspEI T'CCGG_A
Cuts at: 0 213 744
Size: 213 531
BsrFI r'CCGG_y
Cuts at: 0 31 70 76 744
Size: 31 39 6 668
Fragments arranged by size:
668 39 31 6
BstYI r'GATC_y
Cuts at: 0 12 744
Size: 12 732
ClaI AT'CG_AT
Cuts at: 0 303 744
Size: 303 441
DraIII CAC_nnn'GTG
Cuts at: 0 629 744
Size: 629 115
EaeI y'GGCC_r
Cuts at: 0 68 744
Size: 68 676
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 724 744
Size: 571 153 20
Fragments arranged by size:
571 153 20
EcoO109I rG'GnC_Cy
Cuts at: 0 182 729 744
Size: 182 547 15
Fragments arranged by size:
547 182 15
HaeII r_GCGC'y
Cuts at: 0 26 38 77 176 744
Size: 26 12 39 99 568
Fragments arranged by size:
568 99 39 26 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 744
Size: 598 32 114
Fragments arranged by size:
598 114 32
HindIII A'AGCT_T
Cuts at: 0 497 744
Size: 497 247
KasI G'GCGC_C
Cuts at: 0 22 34 73 172 744
Size: 22 12 39 99 572
Fragments arranged by size:
572 99 39 22 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 744
Size: 292 452
MspA1I CmG'CkG
Cuts at: 0 735 744
Size: 735 9
NaeI GCC'GGC
Cuts at: 0 33 72 78 744
Size: 33 39 6 666
Fragments arranged by size:
666 39 33 6
NarI GG'CG_CC
Cuts at: 0 23 35 74 173 744
Size: 23 12 39 99 571
Fragments arranged by size:
571 99 39 23 12
NgoMIV G'CCGG_C
Cuts at: 0 31 70 76 744
Size: 31 39 6 668
Fragments arranged by size:
668 39 31 6
NspI r_CATG'y
Cuts at: 0 702 744
Size: 702 42
PfoI T'CCnGG_A
Cuts at: 0 161 744
Size: 161 583
PspOMI G'GGCC_C
Cuts at: 0 181 744
Size: 181 563
SfcI C'TryA_G
Cuts at: 0 460 561 744
Size: 460 101 183
Fragments arranged by size:
460 183 101
SfoI GGC'GCC
Cuts at: 0 24 36 75 174 744
Size: 24 12 39 99 570
Fragments arranged by size:
570 99 39 24 12
SgrAI Cr'CCGG_yG
Cuts at: 0 76 744
Size: 76 668
SmaI CCC'GGG
Cuts at: 0 180 744
Size: 180 564
SmlI C'TyrA_G
Cuts at: 0 721 744
Size: 721 23
SphI G_CATG'C
Cuts at: 0 702 744
Size: 702 42
StuI AGG'CCT
Cuts at: 0 151 744
Size: 151 593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 259 626 744
Size: 259 367 118
Fragments arranged by size:
367 259 118
XmaI C'CCGG_G
Cuts at: 0 178 744
Size: 178 566
Enzymes that do cut:
AhdI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BciVI BglI BlpI Bme1580I BmgBI BpuEI
BsaHI BsaWI BseYI BsiHKAI Bsp1286I BspEI BsrFI BstYI
ClaI DraIII EaeI EcoNI EcoO109I HaeII Hin4I HindIII
KasI MmeI MspA1I NaeI NarI NgoMIV NspI PfoI
PspOMI SfcI SfoI SgrAI SmaI SmlI SphI StuI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BglII
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EagI EarI EciI EcoICRI Eco57MI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MscI MslI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SnaBI SpeI SrfI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI