Restriction map for
pF1KB8223
(Linear) (Six-base) MAPSORT of: KIBB8223.seq Check: 3155 from: 1 to: 816
KIBB8223.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 435 816
Size: 435 381
AflIII A'CryG_T
Cuts at: 0 500 816
Size: 500 316
BanII G_rGCy'C
Cuts at: 0 488 816
Size: 488 328
BbvCI CC'TCA_GC
Cuts at: 0 641 816
Size: 641 175
BglI GCCn_nnn'nGGC
Cuts at: 0 786 816
Size: 786 30
BlpI GC'TnA_GC
Cuts at: 0 566 608 662 816
Size: 566 42 54 154
Fragments arranged by size:
566 154 54 42
Bme1580I G_kGCm'C
Cuts at: 0 101 816
Size: 101 715
BmgBI CAC'GTC
Cuts at: 0 501 816
Size: 501 315
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 666 816
Size: 453 213 150
Fragments arranged by size:
453 213 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 428 787 816
Size: 423 5 359 29
Fragments arranged by size:
423 359 29 5
Bpu10I CC'TnA_GC
Cuts at: 0 45 641 816
Size: 45 596 175
Fragments arranged by size:
596 175 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 816
Size: 364 452
BsaI GGTCTCn'nnnn_
Cuts at: 0 441 816
Size: 441 375
BsaAI yAC'GTr
Cuts at: 0 220 816
Size: 220 596
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 50 80 816
Size: 50 30 736
Fragments arranged by size:
736 50 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 790 816
Size: 790 26
BseYI C'CCAG_C
Cuts at: 0 94 816
Size: 94 722
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 816
Size: 154 662
BsiHKAI G_wGCw'C
Cuts at: 0 800 816
Size: 800 16
Bsp1286I G_dGCh'C
Cuts at: 0 101 488 800 816
Size: 101 387 312 16
Fragments arranged by size:
387 312 101 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 785 816
Size: 435 350 31
Fragments arranged by size:
435 350 31
BstAPI GCAn_nnn'nTGC
Cuts at: 0 795 816
Size: 795 21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 594 681 816
Size: 561 33 87 135
Fragments arranged by size:
561 135 87 33
BstYI r'GATC_y
Cuts at: 0 374 816
Size: 374 442
BtgI C'CryG_G
Cuts at: 0 126 175 816
Size: 126 49 641
Fragments arranged by size:
641 126 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 723 816
Size: 723 93
BtsI GCAGTG_nn'
Cuts at: 0 271 686 816
Size: 271 415 130
Fragments arranged by size:
415 271 130
EaeI y'GGCC_r
Cuts at: 0 626 683 816
Size: 626 57 133
Fragments arranged by size:
626 133 57
EarI CTCTTCn'nnn_
Cuts at: 0 289 816
Size: 289 527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 428 787 816
Size: 423 5 359 29
Fragments arranged by size:
423 359 29 5
FspI TGC'GCA
Cuts at: 0 277 715 816
Size: 277 438 101
Fragments arranged by size:
438 277 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 401 419 433 451 487 519
Size: 95 32 274 18 14 18 36 32
Cuts at: 519 816
Size: 297
Fragments arranged by size:
297 274 95 36 32 32 18 18
14
HincII GTy'rAC
Cuts at: 0 264 816
Size: 264 552
MscI TGG'CCA
Cuts at: 0 685 816
Size: 685 131
MspA1I CmG'CkG
Cuts at: 0 661 784 807 816
Size: 661 123 23 9
Fragments arranged by size:
661 123 23 9
PfoI T'CCnGG_A
Cuts at: 0 491 816
Size: 491 325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 757 789 816
Size: 757 32 27
Fragments arranged by size:
757 32 27
PstI C_TGCA'G
Cuts at: 0 693 732 816
Size: 693 39 84
Fragments arranged by size:
693 84 39
PvuII CAG'CTG
Cuts at: 0 661 807 816
Size: 661 146 9
Fragments arranged by size:
661 146 9
SfcI C'TryA_G
Cuts at: 0 689 728 816
Size: 689 39 88
Fragments arranged by size:
689 88 39
SmlI C'TyrA_G
Cuts at: 0 379 816
Size: 379 437
StyI C'CwwG_G
Cuts at: 0 653 816
Size: 653 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 134 436 816
Size: 134 302 380
Fragments arranged by size:
380 302 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 50 82 263 295 816
Size: 50 32 181 32 521
Fragments arranged by size:
521 181 50 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 816
Size: 594 222
XmnI GAAnn'nnTTC
Cuts at: 0 532 816
Size: 532 284
Enzymes that do cut:
AarI AflIII BanII BbvCI BglI BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BstAPI BstXI BstYI
BtgI BtgZI BtsI EaeI EarI Eco57MI FspI Hin4I
HincII MscI MspA1I PfoI PsrI PstI PvuII SfcI
SmlI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI ZraI