(Linear) (Six-base) MAPSORT of: KIBB8225.seq  Check: 3770  from: 1  to: 426



KIBB8225.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     426 
   Size:        119     307
 
AflIII A'CryG_T

Cuts at:      0     128     426 
   Size:        128     298
 
AvaI C'yCGr_G

Cuts at:      0      50     426 
   Size:         50     376
 
BanII G_rGCy'C

Cuts at:      0      47     119     426 
   Size:         47      72     307

  Fragments arranged by size: 

                 307      72      47
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     188     426 
   Size:        188     238
 
BbvCI CC'TCA_GC

Cuts at:      0      77     426 
   Size:         77     349
 
BfrBI ATG'CAT

Cuts at:      0     379     426 
   Size:        379      47
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     167     426 
   Size:        167     259
 
Bpu10I CC'TnA_GC

Cuts at:      0      77     426 
   Size:         77     349
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     426 
   Size:        373      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     118     148     426 
   Size:        118      30     278

  Fragments arranged by size: 

                 278     118      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     426 
   Size:        119     307
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      47     426 
   Size:         47     379
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     119     426 
   Size:         47      72     307

  Fragments arranged by size: 

                 307      72      47
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     330     426 
   Size:        330      96
 
BtgI C'CryG_G

Cuts at:      0      65     426 
   Size:         65     361
 
EaeI y'GGCC_r

Cuts at:      0     143     170     426 
   Size:        143      27     256

  Fragments arranged by size: 

                 256     143      27
 
EarI CTCTTCn'nnn_

Cuts at:      0     329     426 
   Size:        329      97
 
EcoICRI GAG'CTC

Cuts at:      0     117     426 
   Size:        117     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     426 
   Size:        119     307
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     426 
   Size:        118      32     276

  Fragments arranged by size: 

                 276     118      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     426 
   Size:        353      73
 
MscI TGG'CCA

Cuts at:      0     145     426 
   Size:        145     281
 
MspA1I CmG'CkG

Cuts at:      0      76     426 
   Size:         76     350
 
NsiI A_TGCA'T

Cuts at:      0     381     426 
   Size:        381      45
 
NspI r_CATG'y

Cuts at:      0     132     426 
   Size:        132     294
 
PciI A'CATG_T

Cuts at:      0     128     426 
   Size:        128     298
 
PfoI T'CCnGG_A

Cuts at:      0     194     426 
   Size:        194     232
 
PstI C_TGCA'G

Cuts at:      0     305     426 
   Size:        305     121
 
PvuII CAG'CTG

Cuts at:      0      76     426 
   Size:         76     350
 
SacI G_AGCT'C

Cuts at:      0     119     426 
   Size:        119     307
 
SapI GCTCTTCn'nnn_

Cuts at:      0     329     426 
   Size:        329      97
 
SfcI C'TryA_G

Cuts at:      0     301     426 
   Size:        301     125
 
SmlI C'TyrA_G

Cuts at:      0     352     426 
   Size:        352      74
 
TatI w'GTAC_w

Cuts at:      0     404     426 
   Size:        404      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     292     426 
   Size:        292     134
 

 Enzymes that do cut:

     AcuI   AflIII     AvaI    BanII     BbsI    BbvCI    BfrBI     BmrI
   Bpu10I    BpuEI    BsaXI  BsiHKAI    BsmBI Bsp1286I    BspMI     BtgI
     EaeI     EarI  EcoICRI  Eco57MI    Hin4I     MmeI     MscI   MspA1I
     NsiI     NspI     PciI     PfoI     PstI    PvuII     SacI     SapI
     SfcI     SmlI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI