(Linear) (Six-base) MAPSORT of: KIBB8227.seq  Check: 2625  from: 1  to: 555



KIBB8227.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     277     555 
   Size:        277     278
 
AvaI C'yCGr_G

Cuts at:      0     382     555 
   Size:        382     173
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10     555 
   Size:         10     545
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     134     555 
   Size:        134     421
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     555 
   Size:        437     118
 
BsaHI Gr'CG_yC

Cuts at:      0     365     555 
   Size:        365     190
 
BsaWI w'CCGG_w

Cuts at:      0     259     506     555 
   Size:        259     247      49

  Fragments arranged by size: 

                 259     247      49
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     158     188     555 
   Size:        158      30     367

  Fragments arranged by size: 

                 367     158      30
 
BseYI C'CCAG_C

Cuts at:      0      42     145     555 
   Size:         42     103     410

  Fragments arranged by size: 

                 410     103      42
 
BsmI GAATG_Cn'

Cuts at:      0     516     555 
   Size:        516      39
 
BsrDI GCAATG_nn'

Cuts at:      0     434     555 
   Size:        434     121
 
BtgI C'CryG_G

Cuts at:      0       6     410     555 
   Size:          6     404     145

  Fragments arranged by size: 

                 404     145       6
 
BtsI GCAGTG_nn'

Cuts at:      0     119     327     555 
   Size:        119     208     228

  Fragments arranged by size: 

                 228     208     119
 
EarI CTCTTCn'nnn_

Cuts at:      0      37     350     555 
   Size:         37     313     205

  Fragments arranged by size: 

                 313     205      37
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     555 
   Size:        437     118
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     477     555 
   Size:        477      78
 
HaeII r_GCGC'y

Cuts at:      0      25     555 
   Size:         25     530
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     216     248     555 
   Size:        216      32     307

  Fragments arranged by size: 

                 307     216      32
 
MslI CAynn'nnrTG

Cuts at:      0     509     555 
   Size:        509      46
 
MspA1I CmG'CkG

Cuts at:      0       8     529     555 
   Size:          8     521      26

  Fragments arranged by size: 

                 521      26       8
 
PvuII CAG'CTG

Cuts at:      0     529     555 
   Size:        529      26
 
SacII CC_GC'GG

Cuts at:      0       9     555 
   Size:          9     546
 
SapI GCTCTTCn'nnn_

Cuts at:      0      37     555 
   Size:         37     518
 
SmaI CCC'GGG

Cuts at:      0     384     555 
   Size:        384     171
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      34     231     555 
   Size:         34     197     324

  Fragments arranged by size: 

                 324     197      34
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     238     270     555 
   Size:        238      32     285

  Fragments arranged by size: 

                 285     238      32
 
XmaI C'CCGG_G

Cuts at:      0     382     555 
   Size:        382     173
 

 Enzymes that do cut:

    AlwNI     AvaI     BglI     BmrI     BpmI    BsaHI    BsaWI    BsaXI
    BseYI     BsmI    BsrDI     BtgI     BtsI     EarI  Eco57MI     FalI
    HaeII    Hin4I     MslI   MspA1I    PvuII    SacII     SapI     SmaI
    TaqII     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI