Restriction map for
pF1KB8232
(Linear) (Six-base) MAPSORT of: KIBB8232.seq Check: 257 from: 1 to: 948
KIBB8232.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 0 7 241 504 948
Size: 7 234 263 444
Fragments arranged by size:
444 263 234 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 948
Size: 472 476
AfeI AGC'GCT
Cuts at: 0 602 948
Size: 602 346
AflIII A'CryG_T
Cuts at: 0 40 948
Size: 40 908
AhdI GACnn_n'nnGTC
Cuts at: 0 642 948
Size: 642 306
AleI CACnn'nnGTG
Cuts at: 0 925 948
Size: 925 23
AlwNI CAG_nnn'CTG
Cuts at: 0 268 653 948
Size: 268 385 295
Fragments arranged by size:
385 295 268
ApoI r'AATT_y
Cuts at: 0 283 554 913 948
Size: 283 271 359 35
Fragments arranged by size:
359 283 271 35
AvaI C'yCGr_G
Cuts at: 0 194 881 948
Size: 194 687 67
Fragments arranged by size:
687 194 67
AvrII C'CTAG_G
Cuts at: 0 520 948
Size: 520 428
BanII G_rGCy'C
Cuts at: 0 748 888 948
Size: 748 140 60
Fragments arranged by size:
748 140 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 661 948
Size: 661 287
BfrBI ATG'CAT
Cuts at: 0 349 948
Size: 349 599
BglI GCCn_nnn'nGGC
Cuts at: 0 787 948
Size: 787 161
BglII A'GATC_T
Cuts at: 0 891 948
Size: 891 57
Bme1580I G_kGCm'C
Cuts at: 0 262 948
Size: 262 686
BmrI ACTGGGnnnn_n'
Cuts at: 0 641 919 948
Size: 641 278 29
Fragments arranged by size:
641 278 29
BsaHI Gr'CG_yC
Cuts at: 0 4 948
Size: 4 944
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 756 948
Size: 756 192
BsmI GAATG_Cn'
Cuts at: 0 349 351 948
Size: 349 2 597
Fragments arranged by size:
597 349 2
Bsp1286I G_dGCh'C
Cuts at: 0 262 748 888 948
Size: 262 486 140 60
Fragments arranged by size:
486 262 140 60
BsrDI GCAATG_nn'
Cuts at: 0 174 344 948
Size: 174 170 604
Fragments arranged by size:
604 174 170
BsrFI r'CCGG_y
Cuts at: 0 85 948
Size: 85 863
BstEII G'GTnAC_C
Cuts at: 0 124 948
Size: 124 824
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 756 948
Size: 18 738 192
Fragments arranged by size:
738 192 18
BstYI r'GATC_y
Cuts at: 0 145 186 891 948
Size: 145 41 705 57
Fragments arranged by size:
705 145 57 41
BtgI C'CryG_G
Cuts at: 0 368 948
Size: 368 580
DraI TTT'AAA
Cuts at: 0 531 585 948
Size: 531 54 363
Fragments arranged by size:
531 363 54
EarI CTCTTCn'nnn_
Cuts at: 0 32 224 439 453 948
Size: 32 192 215 14 495
Fragments arranged by size:
495 215 192 32 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 948
Size: 472 476
EcoO109I rG'GnC_Cy
Cuts at: 0 806 948
Size: 806 142
HaeII r_GCGC'y
Cuts at: 0 604 948
Size: 604 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 628 660 864 896 948
Size: 130 32 466 32 204 32 52
Fragments arranged by size:
466 204 130 52 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 683 948
Size: 180 503 265
Fragments arranged by size:
503 265 180
MslI CAynn'nnrTG
Cuts at: 0 56 367 754 925 948
Size: 56 311 387 171 23
Fragments arranged by size:
387 311 171 56 23
MspA1I CmG'CkG
Cuts at: 0 763 796 948
Size: 763 33 152
Fragments arranged by size:
763 152 33
NcoI C'CATG_G
Cuts at: 0 368 948
Size: 368 580
NsiI A_TGCA'T
Cuts at: 0 351 948
Size: 351 597
NspI r_CATG'y
Cuts at: 0 44 948
Size: 44 904
PciI A'CATG_T
Cuts at: 0 40 948
Size: 40 908
PpuMI rG'GwC_Cy
Cuts at: 0 806 948
Size: 806 142
PspXI vC'TCGA_Gb
Cuts at: 0 881 948
Size: 881 67
PvuII CAG'CTG
Cuts at: 0 796 948
Size: 796 152
SapI GCTCTTCn'nnn_
Cuts at: 0 32 439 453 948
Size: 32 407 14 495
Fragments arranged by size:
495 407 32 14
SfcI C'TryA_G
Cuts at: 0 78 473 501 864 902 948
Size: 78 395 28 363 38 46
Fragments arranged by size:
395 363 78 46 38 28
SmlI C'TyrA_G
Cuts at: 0 194 881 948
Size: 194 687 67
Fragments arranged by size:
687 194 67
StyI C'CwwG_G
Cuts at: 0 368 520 948
Size: 368 152 428
Fragments arranged by size:
428 368 152
TatI w'GTAC_w
Cuts at: 0 681 948
Size: 681 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 817 948
Size: 817 131
XhoI C'TCGA_G
Cuts at: 0 194 881 948
Size: 194 687 67
Fragments arranged by size:
687 194 67
XmnI GAAnn'nnTTC
Cuts at: 0 349 515 948
Size: 349 166 433
Fragments arranged by size:
433 349 166
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlwNI ApoI
AvaI AvrII BanII BbsI BfrBI BglI BglII Bme1580I
BmrI BsaHI BseRI BsmI Bsp1286I BsrDI BsrFI BstEII
BstXI BstYI BtgI DraI EarI Eco57MI EcoO109I HaeII
Hin4I MmeI MslI MspA1I NcoI NsiI NspI PciI
PpuMI PspXI PvuII SapI SfcI SmlI StyI TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AlfI AloI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BanI
BbeI BbvCI BcgI BciVI BclI BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XmaI
ZraI