(Linear) (Six-base) MAPSORT of: KIBB8233.seq  Check: 5018  from: 1  to: 447



KIBB8233.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AclI AA'CG_TT

Cuts at:      0     363     447 
   Size:        363      84
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     261     447 
   Size:        232      29     186

  Fragments arranged by size: 

                 232     186      29
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     397     429     447 
   Size:        397      32      18

  Fragments arranged by size: 

                 397      32      18
 
ApoI r'AATT_y

Cuts at:      0     100     146     447 
   Size:        100      46     301

  Fragments arranged by size: 

                 301     100      46
 
BbvCI CC'TCA_GC

Cuts at:      0     197     447 
   Size:        197     250
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     160     447 
   Size:         52     108     287

  Fragments arranged by size: 

                 287     108      52
 
Bpu10I CC'TnA_GC

Cuts at:      0     197     447 
   Size:        197     250
 
BsiHKAI G_wGCw'C

Cuts at:      0     233     447 
   Size:        233     214
 
Bsp1286I G_dGCh'C

Cuts at:      0     233     447 
   Size:        233     214
 
BssSI C'ACGA_G

Cuts at:      0     232     447 
   Size:        232     215
 
BstYI r'GATC_y

Cuts at:      0     129     447 
   Size:        129     318
 
Bsu36I CC'TnA_GG

Cuts at:      0      45     447 
   Size:         45     402
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     287     447 
   Size:        257      30     160

  Fragments arranged by size: 

                 257     160      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     160     232     261     447 
   Size:         52     108      72      29     186

  Fragments arranged by size: 

                 186     108      72      52      29
 
EcoRI G'AATT_C

Cuts at:      0     100     447 
   Size:        100     347
 
EcoRV GAT'ATC

Cuts at:      0      15     447 
   Size:         15     432
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     262     447 
   Size:        262     185
 
MspA1I CmG'CkG

Cuts at:      0     213     447 
   Size:        213     234
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     312     447 
   Size:        312     135
 
PshAI GACnn'nnGTC

Cuts at:      0     183     447 
   Size:        183     264
 
PstI C_TGCA'G

Cuts at:      0     171     447 
   Size:        171     276
 
PvuII CAG'CTG

Cuts at:      0     213     447 
   Size:        213     234
 
SfcI C'TryA_G

Cuts at:      0     167     447 
   Size:        167     280
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      68     447 
   Size:         68     379
 
XmnI GAAnn'nnTTC

Cuts at:      0      92     245     447 
   Size:         92     153     202

  Fragments arranged by size: 

                 202     153      92
 

 Enzymes that do cut:

     AclI     AcuI     AloI     ApoI    BbvCI     BpmI   Bpu10I  BsiHKAI
 Bsp1286I    BssSI    BstYI   Bsu36I     EarI  Eco57MI    EcoRI    EcoRV
     FalI   MspA1I    PflMI    PshAI     PstI    PvuII     SfcI    TaqII
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI