(Linear) (Six-base) MAPSORT of: KIBB8243.seq  Check: 8520  from: 1  to: 648



KIBB8243.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     648 
   Size:        415     233
 
ApoI r'AATT_y

Cuts at:      0     100     211     523     648 
   Size:        100     111     312     125

  Fragments arranged by size: 

                 312     125     111     100
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     316     349     648 
   Size:        316      33     299

  Fragments arranged by size: 

                 316     299      33
 
BanII G_rGCy'C

Cuts at:      0      20     648 
   Size:         20     628
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     402     648 
   Size:        402     246
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     430     464     648 
   Size:        430      34     184

  Fragments arranged by size: 

                 430     184      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     309     648 
   Size:        309     339
 
BglII A'GATC_T

Cuts at:      0     318     648 
   Size:        318     330
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     243     648 
   Size:        243     405
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     503     648 
   Size:        503     145
 
BseYI C'CCAG_C

Cuts at:      0     570     648 
   Size:        570      78
 
BsiHKAI G_wGCw'C

Cuts at:      0     596     648 
   Size:        596      52
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     596     648 
   Size:         20     576      52

  Fragments arranged by size: 

                 576      52      20
 
BsrFI r'CCGG_y

Cuts at:      0     199     648 
   Size:        199     449
 
BstYI r'GATC_y

Cuts at:      0     318     648 
   Size:        318     330
 
EaeI y'GGCC_r

Cuts at:      0     201     648 
   Size:        201     447
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     648 
   Size:        415     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     169     648 
   Size:        169     479
 
FspI TGC'GCA

Cuts at:      0       8     648 
   Size:          8     640
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     262     294     606     638     648 
   Size:        262      32     312      32      10

  Fragments arranged by size: 

                 312     262      32      32      10
 
MslI CAynn'nnrTG

Cuts at:      0     262     648 
   Size:        262     386
 
NaeI GCC'GGC

Cuts at:      0     201     648 
   Size:        201     447
 
NgoMIV G'CCGG_C

Cuts at:      0     199     648 
   Size:        199     449
 
PfoI T'CCnGG_A

Cuts at:      0     626     648 
   Size:        626      22
 
PpuMI rG'GwC_Cy

Cuts at:      0     169     648 
   Size:        169     479
 
ScaI AGT'ACT

Cuts at:      0     436     648 
   Size:        436     212
 
StyI C'CwwG_G

Cuts at:      0     125     648 
   Size:        125     523
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     118     648 
   Size:        118     530
 
TatI w'GTAC_w

Cuts at:      0     434     648 
   Size:        434     214
 

 Enzymes that do cut:

     AcuI     ApoI     BaeI    BanII     BbsI     BcgI     BglI    BglII
     BmrI     BsaI    BseYI  BsiHKAI Bsp1286I    BsrFI    BstYI     EaeI
  Eco57MI EcoO109I     FspI    Hin4I     MslI     NaeI   NgoMIV     PfoI
    PpuMI     ScaI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI     BbeI
    BbvCI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI