(Linear) (Six-base) MAPSORT of: KIBB8244.seq  Check: 2643  from: 1  to: 1002



KIBB8244.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1002 
   Size:        208     794
 
AvaI C'yCGr_G

Cuts at:      0     314    1002 
   Size:        314     688
 
BanI G'GyrC_C

Cuts at:      0     550    1002 
   Size:        550     452
 
BbeI G_GCGC'C

Cuts at:      0     554    1002 
   Size:        554     448
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     896    1002 
   Size:        896     106
 
BclI T'GATC_A

Cuts at:      0     269    1002 
   Size:        269     733
 
BfrBI ATG'CAT

Cuts at:      0     201    1002 
   Size:        201     801
 
BglI GCCn_nnn'nGGC

Cuts at:      0     422    1002 
   Size:        422     580
 
Bme1580I G_kGCm'C

Cuts at:      0     307    1002 
   Size:        307     695
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     744    1002 
   Size:        744     258
 
Bpu10I CC'TnA_GC

Cuts at:      0     326    1002 
   Size:        326     676
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     733    1002 
   Size:        733     269
 
BsaHI Gr'CG_yC

Cuts at:      0       7     551    1002 
   Size:          7     544     451

  Fragments arranged by size: 

                 544     451       7
 
BseYI C'CCAG_C

Cuts at:      0     833     837    1002 
   Size:        833       4     165

  Fragments arranged by size: 

                 833     165       4
 
Bsp1286I G_dGCh'C

Cuts at:      0     307    1002 
   Size:        307     695
 
BsrBI CCG'CTC

Cuts at:      0      17    1002 
   Size:         17     985
 
BsrGI T'GTAC_A

Cuts at:      0     645    1002 
   Size:        645     357
 
BtgI C'CryG_G

Cuts at:      0     945    1002 
   Size:        945      57
 
BtsI GCAGTG_nn'

Cuts at:      0     506     648    1002 
   Size:        506     142     354

  Fragments arranged by size: 

                 506     354     142
 
ClaI AT'CG_AT

Cuts at:      0      74    1002 
   Size:         74     928
 
EaeI y'GGCC_r

Cuts at:      0      44     381    1002 
   Size:         44     337     621

  Fragments arranged by size: 

                 621     337      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1002 
   Size:        208     794
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     590    1002 
   Size:        590     412
 
EcoO109I rG'GnC_Cy

Cuts at:      0     595    1002 
   Size:        595     407
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     562     913    1002 
   Size:        562     351      89

  Fragments arranged by size: 

                 562     351      89
 
HaeII r_GCGC'y

Cuts at:      0     554    1002 
   Size:        554     448
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158     456     488     868     900    1002 
   Size:        126      32     298      32     380      32     102

  Fragments arranged by size: 

                 380     298     126     102      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     550    1002 
   Size:        550     452
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     538    1002 
   Size:        538     464
 
MscI TGG'CCA

Cuts at:      0      46    1002 
   Size:         46     956
 
MspA1I CmG'CkG

Cuts at:      0     837     920    1002 
   Size:        837      83      82

  Fragments arranged by size: 

                 837      83      82
 
NarI GG'CG_CC

Cuts at:      0     551    1002 
   Size:        551     451
 
NcoI C'CATG_G

Cuts at:      0     945    1002 
   Size:        945      57
 
NdeI CA'TA_TG

Cuts at:      0     339    1002 
   Size:        339     663
 
NsiI A_TGCA'T

Cuts at:      0     203    1002 
   Size:        203     799
 
NspI r_CATG'y

Cuts at:      0      57     205    1002 
   Size:         57     148     797

  Fragments arranged by size: 

                 797     148      57
 
PfoI T'CCnGG_A

Cuts at:      0      10     482    1002 
   Size:         10     472     520

  Fragments arranged by size: 

                 520     472      10
 
PpuMI rG'GwC_Cy

Cuts at:      0     595    1002 
   Size:        595     407
 
PvuII CAG'CTG

Cuts at:      0     837     920    1002 
   Size:        837      83      82

  Fragments arranged by size: 

                 837      83      82
 
ScaI AGT'ACT

Cuts at:      0     160     459    1002 
   Size:        160     299     543

  Fragments arranged by size: 

                 543     299     160
 
SfoI GGC'GCC

Cuts at:      0     552    1002 
   Size:        552     450
 
SmlI C'TyrA_G

Cuts at:      0     712    1002 
   Size:        712     290
 
SphI G_CATG'C

Cuts at:      0     205    1002 
   Size:        205     797
 
SspI AAT'ATT

Cuts at:      0     661    1002 
   Size:        661     341
 
StyI C'CwwG_G

Cuts at:      0     945    1002 
   Size:        945      57
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     726     893    1002 
   Size:        726     167     109

  Fragments arranged by size: 

                 726     167     109
 
TatI w'GTAC_w

Cuts at:      0     158     457     645    1002 
   Size:        158     299     188     357

  Fragments arranged by size: 

                 357     299     188     158
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     273     424    1002 
   Size:        273     151     578

  Fragments arranged by size: 

                 578     273     151
 

 Enzymes that do cut:

     AcuI     AvaI     BanI     BbeI     BbsI     BclI    BfrBI     BglI
 Bme1580I     BmrI   Bpu10I    BpuEI    BsaHI    BseYI Bsp1286I    BsrBI
    BsrGI     BtgI     BtsI     ClaI     EaeI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I     KasI     MmeI     MscI   MspA1I     NarI
     NcoI     NdeI     NsiI     NspI     PfoI    PpuMI    PvuII     ScaI
     SfoI     SmlI     SphI     SspI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MslI
     NaeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI