(Linear) (Six-base) MAPSORT of: KIBB8245.seq  Check: 6329  from: 1  to: 540



KIBB8245.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     418     540 
   Size:        130     288     122

  Fragments arranged by size: 

                 288     130     122
 
AhdI GACnn_n'nnGTC

Cuts at:      0     516     540 
   Size:        516      24
 
ApoI r'AATT_y

Cuts at:      0     296     540 
   Size:        296     244
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       6     540 
   Size:          6     534
 
BsiEI CG_ry'CG

Cuts at:      0     288     540 
   Size:        288     252
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     327     540 
   Size:        327     213
 
BtsI GCAGTG_nn'

Cuts at:      0     362     540 
   Size:        362     178
 
EaeI y'GGCC_r

Cuts at:      0     401     540 
   Size:        401     139
 
EarI CTCTTCn'nnn_

Cuts at:      0      28     446     540 
   Size:         28     418      94

  Fragments arranged by size: 

                 418      94      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     418     540 
   Size:        130     288     122

  Fragments arranged by size: 

                 288     130     122
 
EcoRI G'AATT_C

Cuts at:      0     296     540 
   Size:        296     244
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     258     540 
   Size:        258     282
 
FspI TGC'GCA

Cuts at:      0      55     540 
   Size:         55     485
 
FspAI rTGC'GCAy

Cuts at:      0      55     540 
   Size:         55     485
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     502     534     540 
   Size:        502      32       6

  Fragments arranged by size: 

                 502      32       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     540 
   Size:         48     492
 
MscI TGG'CCA

Cuts at:      0     403     540 
   Size:        403     137
 
MslI CAynn'nnrTG

Cuts at:      0      61     540 
   Size:         61     479
 
PasI CC'CwG_GG

Cuts at:      0     255     540 
   Size:        255     285
 
PstI C_TGCA'G

Cuts at:      0     323     540 
   Size:        323     217
 
PvuI CG_AT'CG

Cuts at:      0     288     540 
   Size:        288     252
 
SfcI C'TryA_G

Cuts at:      0     319     540 
   Size:        319     221
 
StuI AGG'CCT

Cuts at:      0     225     540 
   Size:        225     315
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     120     152     540 
   Size:        120      32     388

  Fragments arranged by size: 

                 388     120      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     296     540 
   Size:        296     244
 

 Enzymes that do cut:

     AcuI     AhdI     ApoI    BseRI    BsiEI   BstAPI     BtsI     EaeI
     EarI  Eco57MI    EcoRI     FalI     FspI    FspAI    Hin4I     MmeI
     MscI     MslI     PasI     PstI     PvuI     SfcI     StuI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI