Restriction map for
pF1KB8249
(Linear) (Six-base) MAPSORT of: KIBB8249.seq Check: 954 from: 1 to: 717
KIBB8249.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 390 567 717
Size: 390 177 150
Fragments arranged by size:
390 177 150
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 31 717
Size: 30 1 686
Fragments arranged by size:
686 30 1
AlwNI CAG_nnn'CTG
Cuts at: 0 508 717
Size: 508 209
ApaLI G'TGCA_C
Cuts at: 0 589 717
Size: 589 128
ApoI r'AATT_y
Cuts at: 0 372 717
Size: 372 345
BanII G_rGCy'C
Cuts at: 0 647 717
Size: 647 70
BclI T'GATC_A
Cuts at: 0 404 717
Size: 404 313
Bme1580I G_kGCm'C
Cuts at: 0 593 717
Size: 593 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 583 613 717
Size: 583 30 104
Fragments arranged by size:
583 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 237 394 688 717
Size: 237 157 294 29
Fragments arranged by size:
294 237 157 29
BsiHKAI G_wGCw'C
Cuts at: 0 593 647 717
Size: 593 54 70
Fragments arranged by size:
593 70 54
Bsp1286I G_dGCh'C
Cuts at: 0 593 647 717
Size: 593 54 70
Fragments arranged by size:
593 70 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 567 713 717
Size: 390 177 146 4
Fragments arranged by size:
390 177 146 4
BsrBI CCG'CTC
Cuts at: 0 678 717
Size: 678 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 508 717
Size: 508 209
BtgI C'CryG_G
Cuts at: 0 121 717
Size: 121 596
BtsI GCAGTG_nn'
Cuts at: 0 236 717
Size: 236 481
EarI CTCTTCn'nnn_
Cuts at: 0 7 578 635 717
Size: 7 571 57 82
Fragments arranged by size:
571 82 57 7
EcoICRI GAG'CTC
Cuts at: 0 645 717
Size: 645 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 31 717
Size: 30 1 686
Fragments arranged by size:
686 30 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 709 717
Size: 709 8
EcoRI G'AATT_C
Cuts at: 0 372 717
Size: 372 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 520 552 717
Size: 238 32 250 32 165
Fragments arranged by size:
250 238 165 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 717
Size: 668 49
MslI CAynn'nnrTG
Cuts at: 0 469 717
Size: 469 248
MspA1I CmG'CkG
Cuts at: 0 564 717
Size: 564 153
NcoI C'CATG_G
Cuts at: 0 121 717
Size: 121 596
NdeI CA'TA_TG
Cuts at: 0 279 717
Size: 279 438
PfoI T'CCnGG_A
Cuts at: 0 601 717
Size: 601 116
PstI C_TGCA'G
Cuts at: 0 480 578 717
Size: 480 98 139
Fragments arranged by size:
480 139 98
PvuII CAG'CTG
Cuts at: 0 564 717
Size: 564 153
SacI G_AGCT'C
Cuts at: 0 647 717
Size: 647 70
SapI GCTCTTCn'nnn_
Cuts at: 0 635 717
Size: 635 82
ScaI AGT'ACT
Cuts at: 0 448 717
Size: 448 269
SfcI C'TryA_G
Cuts at: 0 476 574 717
Size: 476 98 143
Fragments arranged by size:
476 143 98
StuI AGG'CCT
Cuts at: 0 535 700 717
Size: 535 165 17
Fragments arranged by size:
535 165 17
StyI C'CwwG_G
Cuts at: 0 121 717
Size: 121 596
TatI w'GTAC_w
Cuts at: 0 446 717
Size: 446 271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 717
Size: 583 32 102
Fragments arranged by size:
583 102 32
Enzymes that do cut:
AarI AcuI AlwNI ApaLI ApoI BanII BclI Bme1580I
BsaXI BseRI BsiHKAI Bsp1286I BspMI BsrBI BstAPI BtgI
BtsI EarI EcoICRI Eco57MI EcoNI EcoRI Hin4I MmeI
MslI MspA1I NcoI NdeI PfoI PstI PvuII SacI
SapI ScaI SfcI StuI StyI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI