(Linear) (Six-base) MAPSORT of: KIBB8251.seq  Check: 32  from: 1  to: 681



KIBB8251.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AflII C'TTAA_G

Cuts at:      0     589     681 
   Size:        589      92
 
ApoI r'AATT_y

Cuts at:      0      19     283     511     681 
   Size:         19     264     228     170

  Fragments arranged by size: 

                 264     228     170      19
 
Bme1580I G_kGCm'C

Cuts at:      0     128     681 
   Size:        128     553
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     234     264     681 
   Size:        234      30     417

  Fragments arranged by size: 

                 417     234      30
 
BseYI C'CCAG_C

Cuts at:      0     384     681 
   Size:        384     297
 
Bsp1286I G_dGCh'C

Cuts at:      0     128     681 
   Size:        128     553
 
BsrDI GCAATG_nn'

Cuts at:      0     386     452     681 
   Size:        386      66     229

  Fragments arranged by size: 

                 386     229      66
 
BstBI TT'CG_AA

Cuts at:      0     287     681 
   Size:        287     394
 
DraI TTT'AAA

Cuts at:      0     204     632     681 
   Size:        204     428      49

  Fragments arranged by size: 

                 428     204      49
 
EaeI y'GGCC_r

Cuts at:      0      14     681 
   Size:         14     667
 
EcoRI G'AATT_C

Cuts at:      0     283     681 
   Size:        283     398
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     456     488     681 
   Size:        160      32     264      32     193

  Fragments arranged by size: 

                 264     193     160      32      32
 
HindIII A'AGCT_T

Cuts at:      0     570     681 
   Size:        570     111
 
NdeI CA'TA_TG

Cuts at:      0     183     255     681 
   Size:        183      72     426

  Fragments arranged by size: 

                 426     183      72
 
SmlI C'TyrA_G

Cuts at:      0     589     681 
   Size:        589      92
 
SspI AAT'ATT

Cuts at:      0     305     681 
   Size:        305     376
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381     681 
   Size:        381     300
 

 Enzymes that do cut:

    AflII     ApoI Bme1580I    BsaXI    BseYI Bsp1286I    BsrDI    BstBI
     DraI     EaeI    EcoRI    Hin4I  HindIII     NdeI     SmlI     SspI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI