Restriction map for
pF1KB8252
(Linear) (Six-base) MAPSORT of: KIBB8252.seq Check: 4310 from: 1 to: 1005
KIBB8252.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AflIII A'CryG_T
Cuts at: 0 134 716 1005
Size: 134 582 289
Fragments arranged by size:
582 289 134
AhdI GACnn_n'nnGTC
Cuts at: 0 740 1005
Size: 740 265
ApaI G_GGCC'C
Cuts at: 0 805 1005
Size: 805 200
ApoI r'AATT_y
Cuts at: 0 163 949 1005
Size: 163 786 56
Fragments arranged by size:
786 163 56
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 489 522 1005
Size: 489 33 483
Fragments arranged by size:
489 483 33
BanI G'GyrC_C
Cuts at: 0 44 172 1005
Size: 44 128 833
Fragments arranged by size:
833 128 44
BanII G_rGCy'C
Cuts at: 0 330 606 805 1005
Size: 330 276 199 200
Fragments arranged by size:
330 276 200 199
BbeI G_GCGC'C
Cuts at: 0 48 1005
Size: 48 957
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 562 1005
Size: 294 268 443
Fragments arranged by size:
443 294 268
BglI GCCn_nnn'nGGC
Cuts at: 0 991 1005
Size: 991 14
BlpI GC'TnA_GC
Cuts at: 0 468 1005
Size: 468 537
Bme1580I G_kGCm'C
Cuts at: 0 805 1005
Size: 805 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1005
Size: 592 413
Bpu10I CC'TnA_GC
Cuts at: 0 665 1005
Size: 665 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 793 1005
Size: 415 378 212
Fragments arranged by size:
415 378 212
BsaI GGTCTCn'nnnn_
Cuts at: 0 849 1005
Size: 849 156
BsaHI Gr'CG_yC
Cuts at: 0 45 299 793 1005
Size: 45 254 494 212
Fragments arranged by size:
494 254 212 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 856 886 979 1005
Size: 856 30 93 26
Fragments arranged by size:
856 93 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 837 864 1005
Size: 837 27 141
Fragments arranged by size:
837 141 27
BseYI C'CCAG_C
Cuts at: 0 322 889 1005
Size: 322 567 116
Fragments arranged by size:
567 322 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 1005
Size: 438 567
BsiHKAI G_wGCw'C
Cuts at: 0 836 1005
Size: 836 169
Bsp1286I G_dGCh'C
Cuts at: 0 330 606 805 836 1005
Size: 330 276 199 31 169
Fragments arranged by size:
330 276 199 169 31
BspHI T'CATG_A
Cuts at: 0 521 1005
Size: 521 484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 745 762 1005
Size: 745 17 243
Fragments arranged by size:
745 243 17
BsrDI GCAATG_nn'
Cuts at: 0 450 899 1005
Size: 450 449 106
Fragments arranged by size:
450 449 106
BstEII G'GTnAC_C
Cuts at: 0 51 1005
Size: 51 954
BstXI CCAn_nnnn'nTGG
Cuts at: 0 648 1005
Size: 648 357
BstYI r'GATC_y
Cuts at: 0 240 1005
Size: 240 765
BtgI C'CryG_G
Cuts at: 0 156 168 311 597 1005
Size: 156 12 143 286 408
Fragments arranged by size:
408 286 156 143 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 278 1005
Size: 278 727
BtsI GCAGTG_nn'
Cuts at: 0 542 707 1005
Size: 542 165 298
Fragments arranged by size:
542 298 165
DraIII CAC_nnn'GTG
Cuts at: 0 840 1005
Size: 840 165
DrdI GACnn_nn'nnGTC
Cuts at: 0 919 1005
Size: 919 86
EaeI y'GGCC_r
Cuts at: 0 479 594 1005
Size: 479 115 411
Fragments arranged by size:
479 411 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1005
Size: 592 413
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 1005
Size: 481 524
EcoO109I rG'GnC_Cy
Cuts at: 0 801 802 1005
Size: 801 1 203
Fragments arranged by size:
801 203 1
HaeII r_GCGC'y
Cuts at: 0 48 275 638 1005
Size: 48 227 363 367
Fragments arranged by size:
367 363 227 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 726 758 856 888 1005
Size: 556 32 138 32 98 32 117
Fragments arranged by size:
556 138 117 98 32 32 32
HincII GTy'rAC
Cuts at: 0 24 1005
Size: 24 981
HindIII A'AGCT_T
Cuts at: 0 196 1005
Size: 196 809
KasI G'GCGC_C
Cuts at: 0 44 1005
Size: 44 961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1005
Size: 776 229
MscI TGG'CCA
Cuts at: 0 481 1005
Size: 481 524
MslI CAynn'nnrTG
Cuts at: 0 310 400 688 1005
Size: 310 90 288 317
Fragments arranged by size:
317 310 288 90
MspA1I CmG'CkG
Cuts at: 0 599 1005
Size: 599 406
NarI GG'CG_CC
Cuts at: 0 45 1005
Size: 45 960
NcoI C'CATG_G
Cuts at: 0 156 168 311 1005
Size: 156 12 143 694
Fragments arranged by size:
694 156 143 12
NspI r_CATG'y
Cuts at: 0 138 1005
Size: 138 867
PciI A'CATG_T
Cuts at: 0 134 1005
Size: 134 871
PfoI T'CCnGG_A
Cuts at: 0 230 574 1005
Size: 230 344 431
Fragments arranged by size:
431 344 230
PshAI GACnn'nnGTC
Cuts at: 0 529 1005
Size: 529 476
PspOMI G'GGCC_C
Cuts at: 0 801 1005
Size: 801 204
SacII CC_GC'GG
Cuts at: 0 600 1005
Size: 600 405
SexAI A'CCwGG_T
Cuts at: 0 836 1005
Size: 836 169
SfcI C'TryA_G
Cuts at: 0 957 1005
Size: 957 48
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 991 1005
Size: 991 14
SfoI GGC'GCC
Cuts at: 0 46 1005
Size: 46 959
SmlI C'TyrA_G
Cuts at: 0 430 808 1005
Size: 430 378 197
Fragments arranged by size:
430 378 197
StyI C'CwwG_G
Cuts at: 0 156 168 311 482 641 998 1005
Size: 156 12 143 171 159 357 7
Fragments arranged by size:
357 171 159 156 143 12 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 169 388 565 883 1005
Size: 169 219 177 318 122
Fragments arranged by size:
318 219 177 169 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 979 1005
Size: 979 26
XbaI T'CTAG_A
Cuts at: 0 744 1005
Size: 744 261
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 499 720 1005
Size: 393 106 221 285
Fragments arranged by size:
393 285 221 106
Enzymes that do cut:
AflIII AhdI ApaI ApoI BaeI BanI BanII BbeI
BbsI BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PciI PfoI PshAI PspOMI SacII SexAI
SfcI SfiI SfoI SmlI StyI TaqII TstI XbaI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XhoI
XmaI XmnI ZraI