(Linear) (Six-base) MAPSORT of: KIBB8253.seq Check: 205 from: 1 to: 228
KIBB8253.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
ApoI r'AATT_y
Cuts at: 0 102 228
Size: 102 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 228
Size: 172 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 228
Size: 40 188
BtsI GCAGTG_nn'
Cuts at: 0 24 228
Size: 24 204
EcoRI G'AATT_C
Cuts at: 0 102 228
Size: 102 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 36 68 228
Size: 36 32 160
Fragments arranged by size:
160 36 32
SmlI C'TyrA_G
Cuts at: 0 55 228
Size: 55 173
Enzymes that do cut:
ApoI BmrI BpuEI BtsI EcoRI Hin4I SmlI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI