(Linear) (Six-base) MAPSORT of: KIBB8253.seq Check: 205 from: 1 to: 228 KIBB8253.orf With 182 enzymes: SgfI * March 2, 2010 14:28 .. ApoI r'AATT_y Cuts at: 0 102 228 Size: 102 126 BmrI ACTGGGnnnn_n' Cuts at: 0 172 228 Size: 172 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 40 228 Size: 40 188 BtsI GCAGTG_nn' Cuts at: 0 24 228 Size: 24 204 EcoRI G'AATT_C Cuts at: 0 102 228 Size: 102 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 36 68 228 Size: 36 32 160 Fragments arranged by size: 160 36 32 SmlI C'TyrA_G Cuts at: 0 55 228 Size: 55 173 Enzymes that do cut: ApoI BmrI BpuEI BtsI EcoRI Hin4I SmlI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI