(Linear) (Six-base) MAPSORT of: KIBB8254.seq  Check: 2748  from: 1  to: 576



KIBB8254.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     406     576 
   Size:        406     170
 
AgeI A'CCGG_T

Cuts at:      0     147     576 
   Size:        147     429
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     546     576 
   Size:        546      30
 
BanI G'GyrC_C

Cuts at:      0     305     576 
   Size:        305     271
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     162     302     576 
   Size:        162     140     274

  Fragments arranged by size: 

                 274     162     140
 
BclI T'GATC_A

Cuts at:      0      59     576 
   Size:         59     517
 
BfrBI ATG'CAT

Cuts at:      0      79     576 
   Size:         79     497
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     544     576 
   Size:        544      32
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     484     576 
   Size:        107     377      92

  Fragments arranged by size: 

                 377     107      92
 
Bpu10I CC'TnA_GC

Cuts at:      0     437     576 
   Size:        437     139
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     576 
   Size:        478      98
 
BsaWI w'CCGG_w

Cuts at:      0     147     576 
   Size:        147     429
 
BsiEI CG_ry'CG

Cuts at:      0     377     576 
   Size:        377     199
 
BsiHKAI G_wGCw'C

Cuts at:      0     472     576 
   Size:        472     104
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      23     576 
   Size:         23     553
 
Bsp1286I G_dGCh'C

Cuts at:      0     472     576 
   Size:        472     104
 
BsrFI r'CCGG_y

Cuts at:      0     147     576 
   Size:        147     429
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     132     576 
   Size:        132     444
 
Bsu36I CC'TnA_GG

Cuts at:      0     296     576 
   Size:        296     280
 
BtgI C'CryG_G

Cuts at:      0     431     576 
   Size:        431     145
 
EarI CTCTTCn'nnn_

Cuts at:      0     544     550     576 
   Size:        544       6      26

  Fragments arranged by size: 

                 544      26       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     406     484     576 
   Size:        107     299      78      92

  Fragments arranged by size: 

                 299     107      92      78
 
HaeII r_GCGC'y

Cuts at:      0     478     576 
   Size:        478      98
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      80     112     576 
   Size:         80      32     464

  Fragments arranged by size: 

                 464      80      32
 
MslI CAynn'nnrTG

Cuts at:      0     334     576 
   Size:        334     242
 
MspA1I CmG'CkG

Cuts at:      0     175     576 
   Size:        175     401
 
NcoI C'CATG_G

Cuts at:      0     431     576 
   Size:        431     145
 
NsiI A_TGCA'T

Cuts at:      0      81     576 
   Size:         81     495
 
PfoI T'CCnGG_A

Cuts at:      0      84     576 
   Size:         84     492
 
PvuI CG_AT'CG

Cuts at:      0     377     576 
   Size:        377     199
 
PvuII CAG'CTG

Cuts at:      0     175     576 
   Size:        175     401
 
SfcI C'TryA_G

Cuts at:      0      38     576 
   Size:         38     538
 
SmlI C'TyrA_G

Cuts at:      0     493     576 
   Size:        493      83
 
StuI AGG'CCT

Cuts at:      0     491     576 
   Size:        491      85
 
StyI C'CwwG_G

Cuts at:      0     431     576 
   Size:        431     145
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     134     166     576 
   Size:        134      32     410

  Fragments arranged by size: 

                 410     134      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      15     333     576 
   Size:         15     318     243

  Fragments arranged by size: 

                 318     243      15
 

 Enzymes that do cut:

     AcuI     AgeI     AlfI     BanI    BciVI     BclI    BfrBI     BplI
     BpmI   Bpu10I    BpuEI    BsaWI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BsrFI    BstXI   Bsu36I     BtgI     EarI  Eco57MI    HaeII    Hin4I
     MslI   MspA1I     NcoI     NsiI     PfoI     PvuI    PvuII     SfcI
     SmlI     StuI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI