Restriction map for
pF1KB8258
(Linear) (Six-base) MAPSORT of: KIBB8258.seq Check: 2419 from: 1 to: 717
KIBB8258.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 420 717
Size: 420 297
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 491 584 717
Size: 491 93 133
Fragments arranged by size:
491 133 93
AlwNI CAG_nnn'CTG
Cuts at: 0 250 268 717
Size: 250 18 449
Fragments arranged by size:
449 250 18
ApoI r'AATT_y
Cuts at: 0 688 717
Size: 688 29
AvaI C'yCGr_G
Cuts at: 0 23 717
Size: 23 694
BanII G_rGCy'C
Cuts at: 0 499 717
Size: 499 218
BbsI GAAGACnn'nnnn_
Cuts at: 0 383 717
Size: 383 334
BlpI GC'TnA_GC
Cuts at: 0 243 717
Size: 243 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 84 107 176 717
Size: 84 23 69 541
Fragments arranged by size:
541 84 69 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 138 591 717
Size: 53 85 453 126
Fragments arranged by size:
453 126 85 53
Bpu10I CC'TnA_GC
Cuts at: 0 237 717
Size: 237 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 717
Size: 401 316
BsaI GGTCTCn'nnnn_
Cuts at: 0 668 717
Size: 668 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 416 446 717
Size: 416 30 271
Fragments arranged by size:
416 271 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 629 717
Size: 629 88
BseYI C'CCAG_C
Cuts at: 0 407 717
Size: 407 310
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 717
Size: 139 578
BsiEI CG_ry'CG
Cuts at: 0 219 717
Size: 219 498
BsiHKAI G_wGCw'C
Cuts at: 0 499 717
Size: 499 218
Bsp1286I G_dGCh'C
Cuts at: 0 499 717
Size: 499 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 420 717
Size: 420 297
BsrBI CCG'CTC
Cuts at: 0 597 657 717
Size: 597 60 60
Fragments arranged by size:
597 60 60
BsrDI GCAATG_nn'
Cuts at: 0 635 717
Size: 635 82
BsrFI r'CCGG_y
Cuts at: 0 42 717
Size: 42 675
BstAPI GCAn_nnn'nTGC
Cuts at: 0 227 637 717
Size: 227 410 80
Fragments arranged by size:
410 227 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 717
Size: 111 606
BtgI C'CryG_G
Cuts at: 0 590 717
Size: 590 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 162 384 717
Size: 162 222 333
Fragments arranged by size:
333 222 162
BtsI GCAGTG_nn'
Cuts at: 0 566 717
Size: 566 151
EaeI y'GGCC_r
Cuts at: 0 113 587 717
Size: 113 474 130
Fragments arranged by size:
474 130 113
EarI CTCTTCn'nnn_
Cuts at: 0 545 717
Size: 545 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 613 717
Size: 163 450 104
Fragments arranged by size:
450 163 104
EcoICRI GAG'CTC
Cuts at: 0 497 717
Size: 497 220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 138 591 717
Size: 53 85 453 126
Fragments arranged by size:
453 126 85 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 419 717
Size: 349 70 298
Fragments arranged by size:
349 298 70
EcoO109I rG'GnC_Cy
Cuts at: 0 170 411 717
Size: 170 241 306
Fragments arranged by size:
306 241 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 605 637 717
Size: 605 32 80
Fragments arranged by size:
605 80 32
MscI TGG'CCA
Cuts at: 0 589 717
Size: 589 128
MslI CAynn'nnrTG
Cuts at: 0 109 115 717
Size: 109 6 602
Fragments arranged by size:
602 109 6
MspA1I CmG'CkG
Cuts at: 0 247 717
Size: 247 470
NcoI C'CATG_G
Cuts at: 0 590 717
Size: 590 127
PfoI T'CCnGG_A
Cuts at: 0 490 717
Size: 490 227
PpuMI rG'GwC_Cy
Cuts at: 0 170 411 717
Size: 170 241 306
Fragments arranged by size:
306 241 170
PvuII CAG'CTG
Cuts at: 0 247 717
Size: 247 470
SacI G_AGCT'C
Cuts at: 0 499 717
Size: 499 218
SanDI GG'GwC_CC
Cuts at: 0 411 717
Size: 411 306
ScaI AGT'ACT
Cuts at: 0 665 717
Size: 665 52
SmlI C'TyrA_G
Cuts at: 0 416 717
Size: 416 301
StyI C'CwwG_G
Cuts at: 0 545 590 717
Size: 545 45 127
Fragments arranged by size:
545 127 45
TatI w'GTAC_w
Cuts at: 0 434 663 717
Size: 434 229 54
Fragments arranged by size:
434 229 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 717
Size: 416 32 269
Fragments arranged by size:
416 269 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 44 717
Size: 44 673
Enzymes that do cut:
AarI AlfI AlwNI ApoI AvaI BanII BbsI BlpI
BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BtgI BtgZI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I MscI MslI MspA1I
NcoI PfoI PpuMI PvuII SacI SanDI ScaI SmlI
StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI