(Linear) (Six-base) MAPSORT of: KIBB8262.seq  Check: 1658  from: 1  to: 573



KIBB8262.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     318     573 
   Size:        318     255
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      43      75     573 
   Size:         43      32     498

  Fragments arranged by size: 

                 498      43      32
 
AvaI C'yCGr_G

Cuts at:      0       9     573 
   Size:          9     564
 
BanII G_rGCy'C

Cuts at:      0      10     191     573 
   Size:         10     181     382

  Fragments arranged by size: 

                 382     181      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     219     383     573 
   Size:        219     164     190

  Fragments arranged by size: 

                 219     190     164
 
Bme1580I G_kGCm'C

Cuts at:      0     153     161     573 
   Size:        153       8     412

  Fragments arranged by size: 

                 412     153       8
 
BmgBI CAC'GTC

Cuts at:      0     525     573 
   Size:        525      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     524     573 
   Size:        490      34      49

  Fragments arranged by size: 

                 490      49      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     256     382     573 
   Size:        256     126     191

  Fragments arranged by size: 

                 256     191     126
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     573 
   Size:        175     398
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     514     544     573 
   Size:        514      30      29

  Fragments arranged by size: 

                 514      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     487     573 
   Size:        271     216      86

  Fragments arranged by size: 

                 271     216      86
 
BseYI C'CCAG_C

Cuts at:      0     240     573 
   Size:        240     333
 
BsiHKAI G_wGCw'C

Cuts at:      0     191     573 
   Size:        191     382
 
Bsp1286I G_dGCh'C

Cuts at:      0      10     153     161     191     573 
   Size:         10     143       8      30     382

  Fragments arranged by size: 

                 382     143      30      10       8
 
BsrFI r'CCGG_y

Cuts at:      0     386     573 
   Size:        386     187
 
BstYI r'GATC_y

Cuts at:      0      77     276     573 
   Size:         77     199     297

  Fragments arranged by size: 

                 297     199      77
 
EaeI y'GGCC_r

Cuts at:      0      38     573 
   Size:         38     535
 
EcoICRI GAG'CTC

Cuts at:      0     189     573 
   Size:        189     384
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     524     573 
   Size:        490      34      49

  Fragments arranged by size: 

                 490      49      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      25     573 
   Size:         25     548
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     271     573 
   Size:        271     302
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     304     336     573 
   Size:        304      32     237

  Fragments arranged by size: 

                 304     237      32
 
MspA1I CmG'CkG

Cuts at:      0     126     573 
   Size:        126     447
 
NaeI GCC'GGC

Cuts at:      0     388     573 
   Size:        388     185
 
NgoMIV G'CCGG_C

Cuts at:      0     386     573 
   Size:        386     187
 
PfoI T'CCnGG_A

Cuts at:      0     272     467     573 
   Size:        272     195     106

  Fragments arranged by size: 

                 272     195     106
 
PstI C_TGCA'G

Cuts at:      0     223     573 
   Size:        223     350
 
SacI G_AGCT'C

Cuts at:      0     191     573 
   Size:        191     382
 
SfcI C'TryA_G

Cuts at:      0     219     573 
   Size:        219     354
 
SmlI C'TyrA_G

Cuts at:      0     190     573 
   Size:        190     383
 
StyI C'CwwG_G

Cuts at:      0      26     573 
   Size:         26     547
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     514     546     573 
   Size:        514      32      27

  Fragments arranged by size: 

                 514      32      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     573 
   Size:         33     540
 

 Enzymes that do cut:

     AhdI     AloI     AvaI    BanII     BbsI Bme1580I    BmgBI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrFI
    BstYI     EaeI  EcoICRI  Eco57MI    EcoNI     FalI    Hin4I   MspA1I
     NaeI   NgoMIV     PfoI     PstI     SacI     SfcI     SmlI     StyI
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI