Restriction map for
pF1KB8263
(Linear) (Six-base) MAPSORT of: KIBB8263.seq Check: 7216 from: 1 to: 963
KIBB8263.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AvaI C'yCGr_G
Cuts at: 0 496 507 963
Size: 496 11 456
Fragments arranged by size:
496 456 11
BanI G'GyrC_C
Cuts at: 0 511 963
Size: 511 452
BbsI GAAGACnn'nnnn_
Cuts at: 0 279 963
Size: 279 684
BbvCI CC'TCA_GC
Cuts at: 0 819 963
Size: 819 144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 660 694 963
Size: 660 34 269
Fragments arranged by size:
660 269 34
BglI GCCn_nnn'nGGC
Cuts at: 0 47 83 273 963
Size: 47 36 190 690
Fragments arranged by size:
690 190 47 36
Bme1580I G_kGCm'C
Cuts at: 0 514 963
Size: 514 449
BmtI G_CTAG'C
Cuts at: 0 433 963
Size: 433 530
Bpu10I CC'TnA_GC
Cuts at: 0 482 819 963
Size: 482 337 144
Fragments arranged by size:
482 337 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 963
Size: 916 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 525 905 963
Size: 525 380 58
Fragments arranged by size:
525 380 58
BsaBI GATnn'nnATC
Cuts at: 0 466 963
Size: 466 497
BsaWI w'CCGG_w
Cuts at: 0 546 963
Size: 546 417
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 149 152 179 182 963
Size: 149 3 27 3 781
Fragments arranged by size:
781 149 27 3 3
BseYI C'CCAG_C
Cuts at: 0 713 963
Size: 713 250
BsiEI CG_ry'CG
Cuts at: 0 42 963
Size: 42 921
BsmI GAATG_Cn'
Cuts at: 0 750 963
Size: 750 213
Bsp1286I G_dGCh'C
Cuts at: 0 514 963
Size: 514 449
BspEI T'CCGG_A
Cuts at: 0 546 963
Size: 546 417
BsrBI CCG'CTC
Cuts at: 0 422 584 963
Size: 422 162 379
Fragments arranged by size:
422 379 162
BsrDI GCAATG_nn'
Cuts at: 0 689 963
Size: 689 274
BsrGI T'GTAC_A
Cuts at: 0 935 963
Size: 935 28
BstEII G'GTnAC_C
Cuts at: 0 55 318 963
Size: 55 263 645
Fragments arranged by size:
645 263 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 199 330 586 963
Size: 199 131 256 377
Fragments arranged by size:
377 256 199 131
BstYI r'GATC_y
Cuts at: 0 461 963
Size: 461 502
BtgI C'CryG_G
Cuts at: 0 21 60 515 920 963
Size: 21 39 455 405 43
Fragments arranged by size:
455 405 43 39 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 663 963
Size: 663 300
DraIII CAC_nnn'GTG
Cuts at: 0 780 963
Size: 780 183
EaeI y'GGCC_r
Cuts at: 0 39 588 608 963
Size: 39 549 20 355
Fragments arranged by size:
549 355 39 20
EagI C'GGCC_G
Cuts at: 0 39 963
Size: 39 924
EarI CTCTTCn'nnn_
Cuts at: 0 168 355 610 963
Size: 168 187 255 353
Fragments arranged by size:
353 255 187 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 125 963
Size: 125 838
HincII GTy'rAC
Cuts at: 0 890 963
Size: 890 73
MscI TGG'CCA
Cuts at: 0 610 963
Size: 610 353
MslI CAynn'nnrTG
Cuts at: 0 197 275 281 634 682 963
Size: 197 78 6 353 48 281
Fragments arranged by size:
353 281 197 78 48 6
NdeI CA'TA_TG
Cuts at: 0 903 963
Size: 903 60
NheI G'CTAG_C
Cuts at: 0 429 963
Size: 429 534
NspI r_CATG'y
Cuts at: 0 675 963
Size: 675 288
PasI CC'CwG_GG
Cuts at: 0 337 963
Size: 337 626
PflMI CCAn_nnn'nTGG
Cuts at: 0 921 963
Size: 921 42
SexAI A'CCwGG_T
Cuts at: 0 329 963
Size: 329 634
SmlI C'TyrA_G
Cuts at: 0 895 963
Size: 895 68
SphI G_CATG'C
Cuts at: 0 675 963
Size: 675 288
StyI C'CwwG_G
Cuts at: 0 950 963
Size: 950 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 436 671 963
Size: 436 235 292
Fragments arranged by size:
436 292 235
TatI w'GTAC_w
Cuts at: 0 935 963
Size: 935 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 963
Size: 764 32 167
Fragments arranged by size:
764 167 32
XmnI GAAnn'nnTTC
Cuts at: 0 415 963
Size: 415 548
Enzymes that do cut:
AvaI BanI BbsI BbvCI BcgI BglI Bme1580I BmtI
Bpu10I BpuEI BsaI BsaBI BsaWI BseRI BseYI BsiEI
BsmI Bsp1286I BspEI BsrBI BsrDI BsrGI BstEII BstXI
BstYI BtgI BtgZI DraIII EaeI EagI EarI EciI
HincII MscI MslI NdeI NheI NspI PasI PflMI
SexAI SmlI SphI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BciVI BclI BfrBI BglII BlpI BmgBI
BmrI BplI BpmI BsaAI BsaHI BsaXI BsgI BsiHKAI
BsiWI BsmBI BspHI BspMI BsrFI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NcoI NgoMIV
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI