Restriction map for
pF1KB8264
(Linear) (Six-base) MAPSORT of: KIBB8264.seq Check: 6109 from: 1 to: 630
KIBB8264.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 555 630
Size: 555 75
AccI GT'mk_AC
Cuts at: 0 356 630
Size: 356 274
AleI CACnn'nnGTG
Cuts at: 0 68 630
Size: 68 562
AlwNI CAG_nnn'CTG
Cuts at: 0 108 351 390 402 630
Size: 108 243 39 12 228
Fragments arranged by size:
243 228 108 39 12
BamHI G'GATC_C
Cuts at: 0 515 630
Size: 515 115
BanII G_rGCy'C
Cuts at: 0 239 306 630
Size: 239 67 324
Fragments arranged by size:
324 239 67
BmgBI CAC'GTC
Cuts at: 0 444 630
Size: 444 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 630
Size: 581 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 400 630
Size: 130 270 230
Fragments arranged by size:
270 230 130
Bpu10I CC'TnA_GC
Cuts at: 0 437 630
Size: 437 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 630
Size: 4 30 596
Fragments arranged by size:
596 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 28 127 247 301 475 630
Size: 25 3 99 120 54 174 155
Fragments arranged by size:
174 155 120 99 54 25 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 630
Size: 337 293
BsiEI CG_ry'CG
Cuts at: 0 69 157 630
Size: 69 88 473
Fragments arranged by size:
473 88 69
BsiHKAI G_wGCw'C
Cuts at: 0 425 443 630
Size: 425 18 187
Fragments arranged by size:
425 187 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 10 586 630
Size: 10 576 44
Fragments arranged by size:
576 44 10
Bsp1286I G_dGCh'C
Cuts at: 0 239 306 425 443 630
Size: 239 67 119 18 187
Fragments arranged by size:
239 187 119 67 18
BspHI T'CATG_A
Cuts at: 0 449 630
Size: 449 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 593 630
Size: 555 38 37
Fragments arranged by size:
555 38 37
BsrFI r'CCGG_y
Cuts at: 0 23 617 630
Size: 23 594 13
Fragments arranged by size:
594 23 13
BssHII G'CGCG_C
Cuts at: 0 538 630
Size: 538 92
BssSI C'ACGA_G
Cuts at: 0 424 630
Size: 424 206
BstAPI GCAn_nnn'nTGC
Cuts at: 0 390 402 630
Size: 390 12 228
Fragments arranged by size:
390 228 12
BstEII G'GTnAC_C
Cuts at: 0 81 630
Size: 81 549
BstXI CCAn_nnnn'nTGG
Cuts at: 0 70 630
Size: 70 560
BstYI r'GATC_y
Cuts at: 0 515 630
Size: 515 115
BtgI C'CryG_G
Cuts at: 0 69 157 630
Size: 69 88 473
Fragments arranged by size:
473 88 69
EaeI y'GGCC_r
Cuts at: 0 154 630
Size: 154 476
EagI C'GGCC_G
Cuts at: 0 154 630
Size: 154 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 327 630
Size: 327 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 400 630
Size: 130 270 230
Fragments arranged by size:
270 230 130
EcoO109I rG'GnC_Cy
Cuts at: 0 528 630
Size: 528 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 476 508 630
Size: 476 32 122
Fragments arranged by size:
476 122 32
HincII GTy'rAC
Cuts at: 0 357 630
Size: 357 273
MslI CAynn'nnrTG
Cuts at: 0 68 194 262 313 630
Size: 68 126 68 51 317
Fragments arranged by size:
317 126 68 68 51
MspA1I CmG'CkG
Cuts at: 0 57 159 507 630
Size: 57 102 348 123
Fragments arranged by size:
348 123 102 57
NotI GC'GGCC_GC
Cuts at: 0 154 630
Size: 154 476
NspI r_CATG'y
Cuts at: 0 282 630
Size: 282 348
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 630
Size: 108 522
PfoI T'CCnGG_A
Cuts at: 0 377 630
Size: 377 253
PpuMI rG'GwC_Cy
Cuts at: 0 528 630
Size: 528 102
PstI C_TGCA'G
Cuts at: 0 395 630
Size: 395 235
PvuII CAG'CTG
Cuts at: 0 57 507 630
Size: 57 450 123
Fragments arranged by size:
450 123 57
SacII CC_GC'GG
Cuts at: 0 160 630
Size: 160 470
SalI G'TCGA_C
Cuts at: 0 355 630
Size: 355 275
SanDI GG'GwC_CC
Cuts at: 0 528 630
Size: 528 102
SfcI C'TryA_G
Cuts at: 0 391 630
Size: 391 239
SphI G_CATG'C
Cuts at: 0 282 630
Size: 282 348
StyI C'CwwG_G
Cuts at: 0 239 455 630
Size: 239 216 175
Fragments arranged by size:
239 216 175
TatI w'GTAC_w
Cuts at: 0 266 371 630
Size: 266 105 259
Fragments arranged by size:
266 259 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 543 575 630
Size: 543 32 55
Fragments arranged by size:
543 55 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 375 630
Size: 348 27 255
Fragments arranged by size:
348 255 27
Enzymes that do cut:
AarI AccI AleI AlwNI BamHI BanII BmgBI BmrI
BpmI Bpu10I BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BssHII BssSI BstAPI BstEII
BstXI BstYI BtgI EaeI EagI EciI Eco57MI EcoO109I
Hin4I HincII MslI MspA1I NotI NspI PflMI PfoI
PpuMI PstI PvuII SacII SalI SanDI SfcI SphI
StyI TatI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BstBI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI