Restriction map for
pF1KB8265
(Linear) (Six-base) MAPSORT of: KIBB8265.seq Check: 7813 from: 1 to: 1353
KIBB8265.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 203 611 1353
Size: 203 408 742
Fragments arranged by size:
742 408 203
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 379 1353
Size: 141 238 974
Fragments arranged by size:
974 238 141
AgeI A'CCGG_T
Cuts at: 0 388 1353
Size: 388 965
AleI CACnn'nnGTG
Cuts at: 0 1084 1353
Size: 1084 269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 1353
Size: 199 1154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 1353
Size: 71 32 1250
Fragments arranged by size:
1250 71 32
ApaLI G'TGCA_C
Cuts at: 0 384 1353
Size: 384 969
ApoI r'AATT_y
Cuts at: 0 362 760 1353
Size: 362 398 593
Fragments arranged by size:
593 398 362
AvaI C'yCGr_G
Cuts at: 0 188 311 1353
Size: 188 123 1042
Fragments arranged by size:
1042 188 123
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1212 1245 1353
Size: 1212 33 108
Fragments arranged by size:
1212 108 33
BanI G'GyrC_C
Cuts at: 0 241 519 777 1037 1086 1353
Size: 241 278 258 260 49 267
Fragments arranged by size:
278 267 260 258 241 49
BanII G_rGCy'C
Cuts at: 0 68 551 741 1167 1353
Size: 68 483 190 426 186
Fragments arranged by size:
483 426 190 186 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 385 1353
Size: 385 968
BbvCI CC'TCA_GC
Cuts at: 0 1154 1353
Size: 1154 199
BglI GCCn_nnn'nGGC
Cuts at: 0 1046 1353
Size: 1046 307
BlpI GC'TnA_GC
Cuts at: 0 1132 1353
Size: 1132 221
Bme1580I G_kGCm'C
Cuts at: 0 188 320 388 782 1042 1353
Size: 188 132 68 394 260 311
Fragments arranged by size:
394 311 260 188 132 68
BmgBI CAC'GTC
Cuts at: 0 966 1353
Size: 966 387
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1353
Size: 30 1323
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 604 1353
Size: 520 84 749
Fragments arranged by size:
749 520 84
Bpu10I CC'TnA_GC
Cuts at: 0 266 1154 1353
Size: 266 888 199
Fragments arranged by size:
888 266 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1353
Size: 1303 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 1091 1353
Size: 1091 262
BsaAI yAC'GTr
Cuts at: 0 183 1224 1353
Size: 183 1041 129
Fragments arranged by size:
1041 183 129
BsaWI w'CCGG_w
Cuts at: 0 388 1353
Size: 388 965
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 146 1312 1353
Size: 146 1166 41
Fragments arranged by size:
1166 146 41
BseYI C'CCAG_C
Cuts at: 0 59 1353
Size: 59 1294
BsiHKAI G_wGCw'C
Cuts at: 0 68 388 590 1353
Size: 68 320 202 763
Fragments arranged by size:
763 320 202 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 157 472 1353
Size: 157 315 881
Fragments arranged by size:
881 315 157
Bsp1286I G_dGCh'C
Cuts at: 0 68 188 320 388 551 590 741 782
Size: 68 120 132 68 163 39 151 41
Cuts at: 782 1042 1167 1353
Size: 260 125 186
Fragments arranged by size:
260 186 163 151 132 125 120 68
68 41 39
BsrDI GCAATG_nn'
Cuts at: 0 57 992 1148 1353
Size: 57 935 156 205
Fragments arranged by size:
935 205 156 57
BsrFI r'CCGG_y
Cuts at: 0 388 1353
Size: 388 965
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 1353
Size: 56 1297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 97 421 903 1353
Size: 97 324 482 450
Fragments arranged by size:
482 450 324 97
BtgI C'CryG_G
Cuts at: 0 924 957 1353
Size: 924 33 396
Fragments arranged by size:
924 396 33
BtsI GCAGTG_nn'
Cuts at: 0 713 1353
Size: 713 640
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 204 239 1131 1166 1353
Size: 204 35 892 35 187
Fragments arranged by size:
892 204 187 35 35
EaeI y'GGCC_r
Cuts at: 0 27 99 806 1103 1353
Size: 27 72 707 297 250
Fragments arranged by size:
707 297 250 72 27
EarI CTCTTCn'nnn_
Cuts at: 0 262 1353
Size: 262 1091
EcoICRI GAG'CTC
Cuts at: 0 66 1353
Size: 66 1287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 379 520 604 1353
Size: 141 238 141 84 749
Fragments arranged by size:
749 238 141 141 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 1285 1353
Size: 1105 180 68
Fragments arranged by size:
1105 180 68
EcoRI G'AATT_C
Cuts at: 0 362 760 1353
Size: 362 398 593
Fragments arranged by size:
593 398 362
EcoRV GAT'ATC
Cuts at: 0 654 1353
Size: 654 699
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1224 1353
Size: 1224 129
HaeII r_GCGC'y
Cuts at: 0 173 663 1353
Size: 173 490 690
Fragments arranged by size:
690 490 173
HincII GTy'rAC
Cuts at: 0 750 1353
Size: 750 603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1353
Size: 335 1018
MscI TGG'CCA
Cuts at: 0 29 101 808 1105 1353
Size: 29 72 707 297 248
Fragments arranged by size:
707 297 248 72 29
MslI CAynn'nnrTG
Cuts at: 0 1084 1353
Size: 1084 269
MspA1I CmG'CkG
Cuts at: 0 538 1353
Size: 538 815
NcoI C'CATG_G
Cuts at: 0 924 1353
Size: 924 429
NdeI CA'TA_TG
Cuts at: 0 813 1353
Size: 813 540
NspI r_CATG'y
Cuts at: 0 1131 1353
Size: 1131 222
PflMI CCAn_nnn'nTGG
Cuts at: 0 1029 1353
Size: 1029 324
PmlI CAC'GTG
Cuts at: 0 183 1353
Size: 183 1170
PshAI GACnn'nnGTC
Cuts at: 0 635 1353
Size: 635 718
PvuII CAG'CTG
Cuts at: 0 538 1353
Size: 538 815
SacI G_AGCT'C
Cuts at: 0 68 1353
Size: 68 1285
SapI GCTCTTCn'nnn_
Cuts at: 0 262 1353
Size: 262 1091
SexAI A'CCwGG_T
Cuts at: 0 773 1353
Size: 773 580
SfcI C'TryA_G
Cuts at: 0 539 860 1353
Size: 539 321 493
Fragments arranged by size:
539 493 321
SmaI CCC'GGG
Cuts at: 0 190 1353
Size: 190 1163
SmlI C'TyrA_G
Cuts at: 0 1282 1353
Size: 1282 71
SphI G_CATG'C
Cuts at: 0 1131 1353
Size: 1131 222
StuI AGG'CCT
Cuts at: 0 1159 1353
Size: 1159 194
StyI C'CwwG_G
Cuts at: 0 924 1106 1353
Size: 924 182 247
Fragments arranged by size:
924 247 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 374 586 1216 1353
Size: 374 212 630 137
Fragments arranged by size:
630 374 212 137
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 1353
Size: 421 932
XmaI C'CCGG_G
Cuts at: 0 188 1353
Size: 188 1165
XmnI GAAnn'nnTTC
Cuts at: 0 435 1353
Size: 435 918
Enzymes that do cut:
AccI AcuI AgeI AleI AlfI AloI ApaLI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BglI BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI
BstAPI BstXI BtgI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI HaeII HincII MmeI
MscI MslI MspA1I NcoI NdeI NspI PflMI PmlI
PshAI PvuII SacI SapI SexAI SfcI SmaI SmlI
SphI StuI StyI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AlwNI ApaI AscI AseI AsiSI AvrII BamHI BbeI
BcgI BciVI BclI BfrBI BglII BmrI BmtI BsaBI
BsaHI BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoO109I FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI ZraI