Restriction map for
pF1KB8269
(Linear) (Six-base) MAPSORT of: KIBB8269.seq Check: 1886 from: 1 to: 603
KIBB8269.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 603
Size: 516 87
AfeI AGC'GCT
Cuts at: 0 341 603
Size: 341 262
BanI G'GyrC_C
Cuts at: 0 403 603
Size: 403 200
BanII G_rGCy'C
Cuts at: 0 500 601 603
Size: 500 101 2
Fragments arranged by size:
500 101 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 603
Size: 265 338
BciVI GTATCCnnnnn_n'
Cuts at: 0 28 433 603
Size: 28 405 170
Fragments arranged by size:
405 170 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 484 511 603
Size: 170 314 27 92
Fragments arranged by size:
314 170 92 27
BsaAI yAC'GTr
Cuts at: 0 457 603
Size: 457 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 143 173 603
Size: 143 30 430
Fragments arranged by size:
430 143 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 508 603
Size: 295 213 95
Fragments arranged by size:
295 213 95
BsiEI CG_ry'CG
Cuts at: 0 54 432 603
Size: 54 378 171
Fragments arranged by size:
378 171 54
BsiHKAI G_wGCw'C
Cuts at: 0 500 603
Size: 500 103
Bsp1286I G_dGCh'C
Cuts at: 0 500 601 603
Size: 500 101 2
Fragments arranged by size:
500 101 2
BspHI T'CATG_A
Cuts at: 0 93 603
Size: 93 510
BsrGI T'GTAC_A
Cuts at: 0 159 603
Size: 159 444
BstBI TT'CG_AA
Cuts at: 0 540 603
Size: 540 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 400 603
Size: 400 203
Bsu36I CC'TnA_GG
Cuts at: 0 478 603
Size: 478 125
BtgI C'CryG_G
Cuts at: 0 230 393 603
Size: 230 163 210
Fragments arranged by size:
230 210 163
BtsI GCAGTG_nn'
Cuts at: 0 473 603
Size: 473 130
EaeI y'GGCC_r
Cuts at: 0 59 371 603
Size: 59 312 232
Fragments arranged by size:
312 232 59
EcoICRI GAG'CTC
Cuts at: 0 498 603
Size: 498 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 484 511 516 603
Size: 170 314 27 5 87
Fragments arranged by size:
314 170 87 27 5
EcoO109I rG'GnC_Cy
Cuts at: 0 25 378 603
Size: 25 353 225
Fragments arranged by size:
353 225 25
HaeII r_GCGC'y
Cuts at: 0 343 603
Size: 343 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 276 308 603
Size: 276 32 295
Fragments arranged by size:
295 276 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 120 262 603
Size: 74 46 142 341
Fragments arranged by size:
341 142 74 46
MscI TGG'CCA
Cuts at: 0 373 603
Size: 373 230
MslI CAynn'nnrTG
Cuts at: 0 398 603
Size: 398 205
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 603
Size: 570 33
PmlI CAC'GTG
Cuts at: 0 457 603
Size: 457 146
PsiI TTA'TAA
Cuts at: 0 200 603
Size: 200 403
SacI G_AGCT'C
Cuts at: 0 500 603
Size: 500 103
SspI AAT'ATT
Cuts at: 0 72 603
Size: 72 531
StyI C'CwwG_G
Cuts at: 0 21 368 374 603
Size: 21 347 6 229
Fragments arranged by size:
347 229 21 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 446 603
Size: 446 157
TatI w'GTAC_w
Cuts at: 0 159 603
Size: 159 444
XmnI GAAnn'nnTTC
Cuts at: 0 176 508 603
Size: 176 332 95
Fragments arranged by size:
332 176 95
Enzymes that do cut:
AcuI AfeI BanI BanII BbsI BciVI BpmI BsaAI
BsaXI BseRI BsiEI BsiHKAI Bsp1286I BspHI BsrGI BstBI
BstXI Bsu36I BtgI BtsI EaeI EcoICRI Eco57MI EcoO109I
HaeII Hin4I MmeI MscI MslI PflMI PmlI PsiI
SacI SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BseYI BsgI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI