Restriction map for
pF1KB8270
(Linear) (Six-base) MAPSORT of: KIBB8270.seq Check: 7262 from: 1 to: 807
KIBB8270.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 127 807
Size: 127 680
AatII G_ACGT'C
Cuts at: 0 713 807
Size: 713 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 205 766 807
Size: 187 18 561 41
Fragments arranged by size:
561 187 41 18
AgeI A'CCGG_T
Cuts at: 0 548 807
Size: 548 259
AvaI C'yCGr_G
Cuts at: 0 271 703 807
Size: 271 432 104
Fragments arranged by size:
432 271 104
BanI G'GyrC_C
Cuts at: 0 43 155 559 807
Size: 43 112 404 248
Fragments arranged by size:
404 248 112 43
BanII G_rGCy'C
Cuts at: 0 278 577 807
Size: 278 299 230
Fragments arranged by size:
299 278 230
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 98 255 807
Size: 69 29 157 552
Fragments arranged by size:
552 157 69 29
BbvCI CC'TCA_GC
Cuts at: 0 650 807
Size: 650 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 490 807
Size: 456 34 317
Fragments arranged by size:
456 317 34
BglI GCCn_nnn'nGGC
Cuts at: 0 61 807
Size: 61 746
Bme1580I G_kGCm'C
Cuts at: 0 46 158 286 807
Size: 46 112 128 521
Fragments arranged by size:
521 128 112 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 807
Size: 417 390
Bpu10I CC'TnA_GC
Cuts at: 0 650 807
Size: 650 157
BsaHI Gr'CG_yC
Cuts at: 0 710 728 807
Size: 710 18 79
Fragments arranged by size:
710 79 18
BsaWI w'CCGG_w
Cuts at: 0 548 807
Size: 548 259
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 40 70 85 115 807
Size: 40 30 15 30 692
Fragments arranged by size:
692 40 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 807
Size: 103 704
BseYI C'CCAG_C
Cuts at: 0 24 39 807
Size: 24 15 768
Fragments arranged by size:
768 24 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 807
Size: 722 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 352 807
Size: 352 455
Bsp1286I G_dGCh'C
Cuts at: 0 46 158 278 286 577 807
Size: 46 112 120 8 291 230
Fragments arranged by size:
291 230 120 112 46 8
BspHI T'CATG_A
Cuts at: 0 449 807
Size: 449 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 458 807
Size: 127 331 349
Fragments arranged by size:
349 331 127
BsrFI r'CCGG_y
Cuts at: 0 67 494 548 730 807
Size: 67 427 54 182 77
Fragments arranged by size:
427 182 77 67 54
BstEII G'GTnAC_C
Cuts at: 0 666 807
Size: 666 141
BtgI C'CryG_G
Cuts at: 0 48 278 303 807
Size: 48 230 25 504
Fragments arranged by size:
504 230 48 25
EaeI y'GGCC_r
Cuts at: 0 204 287 362 540 617 807
Size: 204 83 75 178 77 190
Fragments arranged by size:
204 190 178 83 77 75
EarI CTCTTCn'nnn_
Cuts at: 0 742 807
Size: 742 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 778 807
Size: 778 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 205 766 807
Size: 187 18 561 41
Fragments arranged by size:
561 187 41 18
EcoO109I rG'GnC_Cy
Cuts at: 0 444 807
Size: 444 363
FspI TGC'GCA
Cuts at: 0 607 807
Size: 607 200
HaeII r_GCGC'y
Cuts at: 0 140 696 807
Size: 140 556 111
Fragments arranged by size:
556 140 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 718 750 807
Size: 85 32 601 32 57
Fragments arranged by size:
601 85 57 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 807
Size: 602 205
MslI CAynn'nnrTG
Cuts at: 0 767 807
Size: 767 40
MspA1I CmG'CkG
Cuts at: 0 161 755 807
Size: 161 594 52
Fragments arranged by size:
594 161 52
NaeI GCC'GGC
Cuts at: 0 69 732 807
Size: 69 663 75
Fragments arranged by size:
663 75 69
NcoI C'CATG_G
Cuts at: 0 278 807
Size: 278 529
NgoMIV G'CCGG_C
Cuts at: 0 67 730 807
Size: 67 663 77
Fragments arranged by size:
663 77 67
PfoI T'CCnGG_A
Cuts at: 0 713 807
Size: 713 94
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 807
Size: 454 32 321
Fragments arranged by size:
454 321 32
SapI GCTCTTCn'nnn_
Cuts at: 0 742 807
Size: 742 65
ScaI AGT'ACT
Cuts at: 0 253 807
Size: 253 554
StyI C'CwwG_G
Cuts at: 0 96 278 569 807
Size: 96 182 291 238
Fragments arranged by size:
291 238 182 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 61 807
Size: 61 746
TatI w'GTAC_w
Cuts at: 0 251 807
Size: 251 556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 40 72 226 258 807
Size: 40 32 154 32 549
Fragments arranged by size:
549 154 40 32 32
ZraI GAC'GTC
Cuts at: 0 711 807
Size: 711 96
Enzymes that do cut:
AarI AatII AcuI AgeI AvaI BanI BanII BbsI
BbvCI BcgI BglI Bme1580I BmrI Bpu10I BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspHI BspMI
BsrFI BstEII BtgI EaeI EarI EciI Eco57MI EcoO109I
FspI HaeII Hin4I MmeI MslI MspA1I NaeI NcoI
NgoMIV PfoI PpiI SapI ScaI StyI TaqII TatI
TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BspEI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI
XmaI XmnI