Restriction map for
pF1KB8272
(Linear) (Six-base) MAPSORT of: KIBB8272.seq Check: 2105 from: 1 to: 483
KIBB8272.orf
With 182 enzymes: SgfI *
June 2, 2011 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 362 483
Size: 362 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 483
Size: 100 383
AflIII A'CryG_T
Cuts at: 0 5 483
Size: 5 478
AlwNI CAG_nnn'CTG
Cuts at: 0 129 483
Size: 129 354
ApaLI G'TGCA_C
Cuts at: 0 349 483
Size: 349 134
AvaI C'yCGr_G
Cuts at: 0 321 483
Size: 321 162
BanI G'GyrC_C
Cuts at: 0 23 483
Size: 23 460
BbeI G_GCGC'C
Cuts at: 0 27 483
Size: 27 456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 54 88 483
Size: 54 34 395
Fragments arranged by size:
395 54 34
BglI GCCn_nnn'nGGC
Cuts at: 0 238 483
Size: 238 245
Bme1580I G_kGCm'C
Cuts at: 0 353 483
Size: 353 130
BmgBI CAC'GTC
Cuts at: 0 6 483
Size: 6 477
BmrI ACTGGGnnnn_n'
Cuts at: 0 178 483
Size: 178 305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 483
Size: 420 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 483
Size: 88 395
BsaHI Gr'CG_yC
Cuts at: 0 24 50 483
Size: 24 26 433
Fragments arranged by size:
433 26 24
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 393 423 483
Size: 393 30 60
Fragments arranged by size:
393 60 30
BseYI C'CCAG_C
Cuts at: 0 213 473 483
Size: 213 260 10
Fragments arranged by size:
260 213 10
BsiHKAI G_wGCw'C
Cuts at: 0 353 483
Size: 353 130
Bsp1286I G_dGCh'C
Cuts at: 0 353 483
Size: 353 130
BspHI T'CATG_A
Cuts at: 0 14 483
Size: 14 469
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 483
Size: 362 121
BsrGI T'GTAC_A
Cuts at: 0 131 483
Size: 131 352
BstBI TT'CG_AA
Cuts at: 0 74 483
Size: 74 409
BstXI CCAn_nnnn'nTGG
Cuts at: 0 412 483
Size: 412 71
BtsI GCAGTG_nn'
Cuts at: 0 336 483
Size: 336 147
EaeI y'GGCC_r
Cuts at: 0 202 377 483
Size: 202 175 106
Fragments arranged by size:
202 175 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 420 483
Size: 100 320 63
Fragments arranged by size:
320 100 63
EcoO109I rG'GnC_Cy
Cuts at: 0 210 258 483
Size: 210 48 225
Fragments arranged by size:
225 210 48
HaeII r_GCGC'y
Cuts at: 0 27 483
Size: 27 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 483
Size: 393 32 58
Fragments arranged by size:
393 58 32
KasI G'GCGC_C
Cuts at: 0 23 483
Size: 23 460
MscI TGG'CCA
Cuts at: 0 379 483
Size: 379 104
MslI CAynn'nnrTG
Cuts at: 0 356 483
Size: 356 127
MspA1I CmG'CkG
Cuts at: 0 126 483
Size: 126 357
NarI GG'CG_CC
Cuts at: 0 24 483
Size: 24 459
NspI r_CATG'y
Cuts at: 0 361 428 483
Size: 361 67 55
Fragments arranged by size:
361 67 55
PflMI CCAn_nnn'nTGG
Cuts at: 0 150 375 483
Size: 150 225 108
Fragments arranged by size:
225 150 108
SfoI GGC'GCC
Cuts at: 0 25 483
Size: 25 458
SmlI C'TyrA_G
Cuts at: 0 103 483
Size: 103 380
StyI C'CwwG_G
Cuts at: 0 53 483
Size: 53 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 482 483
Size: 482 1
TatI w'GTAC_w
Cuts at: 0 131 483
Size: 131 352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 483
Size: 225 258
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaLI AvaI BanI BbeI
BcgI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaHI
BsaXI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrGI BstBI
BstXI BtsI EaeI Eco57MI EcoO109I HaeII Hin4I KasI
MscI MslI MspA1I NarI NspI PflMI SfoI SmlI
StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI