Restriction map for
pF1KB8275
(Linear) (Six-base) MAPSORT of: KIBB8275.seq Check: 9555 from: 1 to: 918
KIBB8275.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 371 918
Size: 371 547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 358 403 602 918
Size: 281 77 45 199 316
Fragments arranged by size:
316 281 199 77 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 918
Size: 30 888
ApoI r'AATT_y
Cuts at: 0 355 548 918
Size: 355 193 370
Fragments arranged by size:
370 355 193
BanI G'GyrC_C
Cuts at: 0 163 918
Size: 163 755
BanII G_rGCy'C
Cuts at: 0 638 837 918
Size: 638 199 81
Fragments arranged by size:
638 199 81
BciVI GTATCCnnnnn_n'
Cuts at: 0 888 918
Size: 888 30
BglI GCCn_nnn'nGGC
Cuts at: 0 91 918
Size: 91 827
BglII A'GATC_T
Cuts at: 0 333 918
Size: 333 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 554 918
Size: 179 375 364
Fragments arranged by size:
375 364 179
Bpu10I CC'TnA_GC
Cuts at: 0 310 918
Size: 310 608
BsaHI Gr'CG_yC
Cuts at: 0 206 918
Size: 206 712
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 74 104 918
Size: 28 46 30 814
Fragments arranged by size:
814 46 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 234 235 830 918
Size: 22 212 1 595 88
Fragments arranged by size:
595 212 88 22 1
BseYI C'CCAG_C
Cuts at: 0 314 918
Size: 314 604
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 918
Size: 360 558
BsiHKAI G_wGCw'C
Cuts at: 0 638 837 918
Size: 638 199 81
Fragments arranged by size:
638 199 81
Bsp1286I G_dGCh'C
Cuts at: 0 638 837 918
Size: 638 199 81
Fragments arranged by size:
638 199 81
BsrFI r'CCGG_y
Cuts at: 0 537 918
Size: 537 381
BssSI C'ACGA_G
Cuts at: 0 836 918
Size: 836 82
BstEII G'GTnAC_C
Cuts at: 0 850 918
Size: 850 68
BstXI CCAn_nnnn'nTGG
Cuts at: 0 739 862 918
Size: 739 123 56
Fragments arranged by size:
739 123 56
BstYI r'GATC_y
Cuts at: 0 333 390 808 918
Size: 333 57 418 110
Fragments arranged by size:
418 333 110 57
BtgI C'CryG_G
Cuts at: 0 86 641 918
Size: 86 555 277
Fragments arranged by size:
555 277 86
DraI TTT'AAA
Cuts at: 0 435 918
Size: 435 483
EarI CTCTTCn'nnn_
Cuts at: 0 578 825 837 918
Size: 578 247 12 81
Fragments arranged by size:
578 247 81 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 161 918
Size: 161 757
EcoICRI GAG'CTC
Cuts at: 0 636 835 918
Size: 636 199 83
Fragments arranged by size:
636 199 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 281 358 403 554 602 918
Size: 179 102 77 45 151 48 316
Fragments arranged by size:
316 179 151 102 77 48 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 398 918
Size: 398 520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 918
Size: 363 555
HaeII r_GCGC'y
Cuts at: 0 182 918
Size: 182 736
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 610 642 918
Size: 610 32 276
Fragments arranged by size:
610 276 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 918
Size: 167 751
MspA1I CmG'CkG
Cuts at: 0 40 112 279 918
Size: 40 72 167 639
Fragments arranged by size:
639 167 72 40
NcoI C'CATG_G
Cuts at: 0 86 641 918
Size: 86 555 277
Fragments arranged by size:
555 277 86
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 918
Size: 30 888
SacI G_AGCT'C
Cuts at: 0 638 837 918
Size: 638 199 81
Fragments arranged by size:
638 199 81
SapI GCTCTTCn'nnn_
Cuts at: 0 825 918
Size: 825 93
ScaI AGT'ACT
Cuts at: 0 601 918
Size: 601 317
StuI AGG'CCT
Cuts at: 0 308 918
Size: 308 610
StyI C'CwwG_G
Cuts at: 0 86 641 918
Size: 86 555 277
Fragments arranged by size:
555 277 86
TatI w'GTAC_w
Cuts at: 0 599 918
Size: 599 319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 918
Size: 74 32 812
Fragments arranged by size:
812 74 32
XbaI T'CTAG_A
Cuts at: 0 811 918
Size: 811 107
Enzymes that do cut:
AccI AcuI AloI ApoI BanI BanII BciVI BglI
BglII BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrFI BssSI BstEII BstXI BstYI BtgI
DraI EarI EciI EcoICRI Eco57MI EcoNI FalI HaeII
Hin4I MmeI MspA1I NcoI PpiI SacI SapI ScaI
StuI StyI TatI TstI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EcoO109I EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XcmI XhoI XmaI XmnI ZraI