Restriction map for
pF1KB8276
(Linear) (Six-base) MAPSORT of: KIBB8276.seq Check: 9580 from: 1 to: 417
KIBB8276.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 417
Size: 40 377
AvaI C'yCGr_G
Cuts at: 0 169 360 417
Size: 169 191 57
Fragments arranged by size:
191 169 57
BamHI G'GATC_C
Cuts at: 0 356 417
Size: 356 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 417
Size: 28 389
BglI GCCn_nnn'nGGC
Cuts at: 0 272 417
Size: 272 145
Bme1580I G_kGCm'C
Cuts at: 0 367 417
Size: 367 50
BmgBI CAC'GTC
Cuts at: 0 137 417
Size: 137 280
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 417
Size: 256 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 417
Size: 117 300
BsiEI CG_ry'CG
Cuts at: 0 267 417
Size: 267 150
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 417
Size: 61 356
Bsp1286I G_dGCh'C
Cuts at: 0 367 417
Size: 367 50
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 212 417
Size: 125 87 205
Fragments arranged by size:
205 125 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 417
Size: 48 369
BstYI r'GATC_y
Cuts at: 0 356 417
Size: 356 61
BtgI C'CryG_G
Cuts at: 0 183 261 267 303 417
Size: 183 78 6 36 114
Fragments arranged by size:
183 114 78 36 6
BtsI GCAGTG_nn'
Cuts at: 0 13 417
Size: 13 404
DraIII CAC_nnn'GTG
Cuts at: 0 87 417
Size: 87 330
EaeI y'GGCC_r
Cuts at: 0 251 264 321 339 417
Size: 251 13 57 18 78
Fragments arranged by size:
251 78 57 18 13
EagI C'GGCC_G
Cuts at: 0 264 417
Size: 264 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 177 417
Size: 177 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 417
Size: 40 377
FspI TGC'GCA
Cuts at: 0 208 417
Size: 208 209
HaeII r_GCGC'y
Cuts at: 0 320 417
Size: 320 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 113 145 341 373 417
Size: 113 32 196 32 44
Fragments arranged by size:
196 113 44 32 32
MscI TGG'CCA
Cuts at: 0 253 417
Size: 253 164
MslI CAynn'nnrTG
Cuts at: 0 46 229 417
Size: 46 183 188
Fragments arranged by size:
188 183 46
MspA1I CmG'CkG
Cuts at: 0 185 263 269 305 417
Size: 185 78 6 36 112
Fragments arranged by size:
185 112 78 36 6
NotI GC'GGCC_GC
Cuts at: 0 264 417
Size: 264 153
PstI C_TGCA'G
Cuts at: 0 281 417
Size: 281 136
SacII CC_GC'GG
Cuts at: 0 186 264 270 306 417
Size: 186 78 6 36 111
Fragments arranged by size:
186 111 78 36 6
SexAI A'CCwGG_T
Cuts at: 0 83 417
Size: 83 334
SfcI C'TryA_G
Cuts at: 0 277 417
Size: 277 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 272 417
Size: 272 145
SmaI CCC'GGG
Cuts at: 0 362 417
Size: 362 55
StuI AGG'CCT
Cuts at: 0 275 417
Size: 275 142
StyI C'CwwG_G
Cuts at: 0 254 417
Size: 254 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 417
Size: 71 32 314
Fragments arranged by size:
314 71 32
Tth111I GACn'n_nGTC
Cuts at: 0 232 417
Size: 232 185
XmaI C'CCGG_G
Cuts at: 0 360 417
Size: 360 57
Enzymes that do cut:
AcuI AvaI BamHI BbsI BglI Bme1580I BmgBI BseRI
BsgI BsiEI BsmBI Bsp1286I BspMI BstXI BstYI BtgI
BtsI DraIII EaeI EagI EciI Eco57MI FspI HaeII
Hin4I MscI MslI MspA1I NotI PstI SacII SexAI
SfcI SfiI SmaI StuI StyI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TatI XbaI
XcmI XhoI XmnI ZraI