Restriction map for
pF1KB8277
(Linear) (Six-base) MAPSORT of: KIBB8277.seq Check: 9023 from: 1 to: 1071
KIBB8277.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 378 1071
Size: 378 693
AccI GT'mk_AC
Cuts at: 0 58 491 1071
Size: 58 433 580
Fragments arranged by size:
580 433 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1071
Size: 334 737
AfeI AGC'GCT
Cuts at: 0 1043 1071
Size: 1043 28
AflIII A'CryG_T
Cuts at: 0 770 950 1071
Size: 770 180 121
Fragments arranged by size:
770 180 121
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 665 666 697 698 1071
Size: 665 1 31 1 373
Fragments arranged by size:
665 373 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 471 1071
Size: 471 600
ApoI r'AATT_y
Cuts at: 0 970 1071
Size: 970 101
AvaI C'yCGr_G
Cuts at: 0 638 1071
Size: 638 433
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 738 771 1071
Size: 738 33 300
Fragments arranged by size:
738 300 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 370 1071
Size: 370 701
BclI T'GATC_A
Cuts at: 0 99 917 1071
Size: 99 818 154
Fragments arranged by size:
818 154 99
BlpI GC'TnA_GC
Cuts at: 0 85 158 1071
Size: 85 73 913
Fragments arranged by size:
913 85 73
Bme1580I G_kGCm'C
Cuts at: 0 722 1071
Size: 722 349
BmtI G_CTAG'C
Cuts at: 0 16 1071
Size: 16 1055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 618 1071
Size: 618 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 438 538 677 712 1071
Size: 358 80 100 139 35 359
Fragments arranged by size:
359 358 139 100 80 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 797 936 1071
Size: 797 139 135
Fragments arranged by size:
797 139 135
BsaBI GATnn'nnATC
Cuts at: 0 697 922 1071
Size: 697 225 149
Fragments arranged by size:
697 225 149
BseYI C'CCAG_C
Cuts at: 0 31 414 1071
Size: 31 383 657
Fragments arranged by size:
657 383 31
BsmI GAATG_Cn'
Cuts at: 0 839 1071
Size: 839 232
BsmBI CGTCTCn'nnnn_
Cuts at: 0 20 334 1071
Size: 20 314 737
Fragments arranged by size:
737 314 20
Bsp1286I G_dGCh'C
Cuts at: 0 722 1071
Size: 722 349
BspHI T'CATG_A
Cuts at: 0 701 1071
Size: 701 370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 378 1071
Size: 378 693
BsrBI CCG'CTC
Cuts at: 0 162 711 1071
Size: 162 549 360
Fragments arranged by size:
549 360 162
BsrDI GCAATG_nn'
Cuts at: 0 252 1071
Size: 252 819
BsrGI T'GTAC_A
Cuts at: 0 503 1071
Size: 503 568
BstEII G'GTnAC_C
Cuts at: 0 213 1071
Size: 213 858
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 1071
Size: 204 867
BstYI r'GATC_y
Cuts at: 0 642 1071
Size: 642 429
BtsI GCAGTG_nn'
Cuts at: 0 945 1071
Size: 945 126
EaeI y'GGCC_r
Cuts at: 0 330 1071
Size: 330 741
EarI CTCTTCn'nnn_
Cuts at: 0 582 658 1071
Size: 582 76 413
Fragments arranged by size:
582 413 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 358 438 538 677 712 1071
Size: 334 24 80 100 139 35 359
Fragments arranged by size:
359 334 139 100 80 35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 1071
Size: 577 494
EcoO109I rG'GnC_Cy
Cuts at: 0 912 1071
Size: 912 159
EcoRI G'AATT_C
Cuts at: 0 970 1071
Size: 970 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 105 339 1071
Size: 63 42 234 732
Fragments arranged by size:
732 234 63 42
HaeII r_GCGC'y
Cuts at: 0 1045 1071
Size: 1045 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 357 389 415 447 896 928 1071
Size: 357 32 26 32 449 32 143
Fragments arranged by size:
449 357 143 32 32 32 26
HincII GTy'rAC
Cuts at: 0 225 1071
Size: 225 846
HindIII A'AGCT_T
Cuts at: 0 398 1071
Size: 398 673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1071
Size: 755 316
MscI TGG'CCA
Cuts at: 0 332 1071
Size: 332 739
NdeI CA'TA_TG
Cuts at: 0 123 1071
Size: 123 948
NheI G'CTAG_C
Cuts at: 0 12 1071
Size: 12 1059
NspI r_CATG'y
Cuts at: 0 774 954 1071
Size: 774 180 117
Fragments arranged by size:
774 180 117
PciI A'CATG_T
Cuts at: 0 770 950 1071
Size: 770 180 121
Fragments arranged by size:
770 180 121
PpuMI rG'GwC_Cy
Cuts at: 0 912 1071
Size: 912 159
PstI C_TGCA'G
Cuts at: 0 476 1071
Size: 476 595
SapI GCTCTTCn'nnn_
Cuts at: 0 582 1071
Size: 582 489
SexAI A'CCwGG_T
Cuts at: 0 827 1071
Size: 827 244
SfcI C'TryA_G
Cuts at: 0 472 1071
Size: 472 599
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 721 1071
Size: 721 350
TatI w'GTAC_w
Cuts at: 0 503 998 1071
Size: 503 495 73
Fragments arranged by size:
503 495 73
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 1071
Size: 411 660
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AloI AlwNI ApoI
AvaI BaeI BbsI BclI BlpI Bme1580I BmtI BplI
BpmI BsaI BsaBI BseYI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrGI BstEII BstXI BstYI BtsI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII MmeI MscI NdeI NheI NspI
PciI PpuMI PstI SapI SexAI SfcI TaqII TatI
XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BanI
BanII BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BmgBI BmrI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsiWI BspEI BsrFI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI