Restriction map for
pF1KB8290
(Linear) (Six-base) MAPSORT of: KIBB8290.seq Check: 2646 from: 1 to: 1683
KIBB8290.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
Acc65I G'GTAC_C
Cuts at: 0 1561 1683
Size: 1561 122
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1216 1400 1683
Size: 760 456 184 283
Fragments arranged by size:
760 456 283 184
AflIII A'CryG_T
Cuts at: 0 66 1683
Size: 66 1617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1065 1097 1683
Size: 1065 32 586
Fragments arranged by size:
1065 586 32
AlwNI CAG_nnn'CTG
Cuts at: 0 892 1683
Size: 892 791
ApoI r'AATT_y
Cuts at: 0 34 1683
Size: 34 1649
AvaI C'yCGr_G
Cuts at: 0 762 1683
Size: 762 921
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1503 1536 1683
Size: 1503 33 147
Fragments arranged by size:
1503 147 33
BanI G'GyrC_C
Cuts at: 0 1302 1561 1683
Size: 1302 259 122
Fragments arranged by size:
1302 259 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 642 782 923 1683
Size: 642 140 141 760
Fragments arranged by size:
760 642 141 140
BbvCI CC'TCA_GC
Cuts at: 0 1468 1683
Size: 1468 215
BciVI GTATCCnnnnn_n'
Cuts at: 0 687 1182 1524 1683
Size: 687 495 342 159
Fragments arranged by size:
687 495 342 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 1313 1511 1683
Size: 1313 198 172
Fragments arranged by size:
1313 198 172
BmtI G_CTAG'C
Cuts at: 0 1574 1683
Size: 1574 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 466 1683
Size: 466 1217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 665 725 1064 1109 1683
Size: 596 69 60 339 45 574
Fragments arranged by size:
596 574 339 69 60 45
Bpu10I CC'TnA_GC
Cuts at: 0 1342 1468 1683
Size: 1342 126 215
Fragments arranged by size:
1342 215 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1391 1683
Size: 643 748 292
Fragments arranged by size:
748 643 292
BsaI GGTCTCn'nnnn_
Cuts at: 0 788 1433 1683
Size: 788 645 250
Fragments arranged by size:
788 645 250
BsaAI yAC'GTr
Cuts at: 0 549 1683
Size: 549 1134
BsaHI Gr'CG_yC
Cuts at: 0 384 1683
Size: 384 1299
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1059 1065 1089 1095 1683
Size: 1059 6 24 6 588
Fragments arranged by size:
1059 588 24 6 6
BseYI C'CCAG_C
Cuts at: 0 477 1019 1683
Size: 477 542 664
Fragments arranged by size:
664 542 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1343 1683
Size: 571 772 340
Fragments arranged by size:
772 571 340
BsiHKAI G_wGCw'C
Cuts at: 0 281 1012 1683
Size: 281 731 671
Fragments arranged by size:
731 671 281
Bsp1286I G_dGCh'C
Cuts at: 0 281 1012 1683
Size: 281 731 671
Fragments arranged by size:
731 671 281
BspHI T'CATG_A
Cuts at: 0 452 750 1683
Size: 452 298 933
Fragments arranged by size:
933 452 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 1683
Size: 107 1576
BsrBI CCG'CTC
Cuts at: 0 447 1683
Size: 447 1236
BsrDI GCAATG_nn'
Cuts at: 0 465 1683
Size: 465 1218
BsrFI r'CCGG_y
Cuts at: 0 1614 1683
Size: 1614 69
BstEII G'GTnAC_C
Cuts at: 0 616 1683
Size: 616 1067
BstXI CCAn_nnnn'nTGG
Cuts at: 0 703 1683
Size: 703 980
BstYI r'GATC_y
Cuts at: 0 390 1683
Size: 390 1293
Bsu36I CC'TnA_GG
Cuts at: 0 1184 1395 1683
Size: 1184 211 288
Fragments arranged by size:
1184 288 211
BtsI GCAGTG_nn'
Cuts at: 0 1260 1331 1683
Size: 1260 71 352
Fragments arranged by size:
1260 352 71
ClaI AT'CG_AT
Cuts at: 0 1416 1683
Size: 1416 267
DraI TTT'AAA
Cuts at: 0 126 1683
Size: 126 1557
DraIII CAC_nnn'GTG
Cuts at: 0 487 1057 1683
Size: 487 570 626
Fragments arranged by size:
626 570 487
EaeI y'GGCC_r
Cuts at: 0 1281 1616 1683
Size: 1281 335 67
Fragments arranged by size:
1281 335 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 665 725 760 1064 1109 1216 1400
Size: 596 69 60 35 304 45 107 184
Cuts at: 1400 1683
Size: 283
Fragments arranged by size:
596 304 283 184 107 69 60 45
35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 1683
Size: 364 1319
EcoRV GAT'ATC
Cuts at: 0 1165 1683
Size: 1165 518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 196 1135 1683
Size: 196 939 548
Fragments arranged by size:
939 548 196
FspI TGC'GCA
Cuts at: 0 241 1683
Size: 241 1442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 745 777 1476 1508 1683
Size: 26 719 32 699 32 175
Fragments arranged by size:
719 699 175 32 32 26
HincII GTy'rAC
Cuts at: 0 711 1683
Size: 711 972
KpnI G_GTAC'C
Cuts at: 0 1565 1683
Size: 1565 118
MfeI C'AATT_G
Cuts at: 0 77 719 1683
Size: 77 642 964
Fragments arranged by size:
964 642 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1129 1177 1683
Size: 961 168 48 506
Fragments arranged by size:
961 506 168 48
MscI TGG'CCA
Cuts at: 0 1283 1683
Size: 1283 400
MslI CAynn'nnrTG
Cuts at: 0 71 1319 1683
Size: 71 1248 364
Fragments arranged by size:
1248 364 71
MspA1I CmG'CkG
Cuts at: 0 889 1467 1683
Size: 889 578 216
Fragments arranged by size:
889 578 216
NheI G'CTAG_C
Cuts at: 0 1570 1683
Size: 1570 113
NspI r_CATG'y
Cuts at: 0 70 1683
Size: 70 1613
PasI CC'CwG_GG
Cuts at: 0 696 1683
Size: 696 987
PciI A'CATG_T
Cuts at: 0 66 1683
Size: 66 1617
PflMI CCAn_nnn'nTGG
Cuts at: 0 892 1683
Size: 892 791
PfoI T'CCnGG_A
Cuts at: 0 1032 1683
Size: 1032 651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1065 1097 1683
Size: 1065 32 586
Fragments arranged by size:
1065 586 32
PstI C_TGCA'G
Cuts at: 0 104 1683
Size: 104 1579
PvuII CAG'CTG
Cuts at: 0 889 1467 1683
Size: 889 578 216
Fragments arranged by size:
889 578 216
SbfI CC_TGCA'GG
Cuts at: 0 104 1683
Size: 104 1579
ScaI AGT'ACT
Cuts at: 0 121 1683
Size: 121 1562
SfcI C'TryA_G
Cuts at: 0 100 854 1683
Size: 100 754 829
Fragments arranged by size:
829 754 100
SmlI C'TyrA_G
Cuts at: 0 622 1406 1683
Size: 622 784 277
Fragments arranged by size:
784 622 277
StyI C'CwwG_G
Cuts at: 0 425 1683
Size: 425 1258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 1620 1683
Size: 56 1564 63
Fragments arranged by size:
1564 63 56
TatI w'GTAC_w
Cuts at: 0 119 1683
Size: 119 1564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 473 505 1100 1132 1683
Size: 473 32 595 32 551
Fragments arranged by size:
595 551 473 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 376 1683
Size: 376 1307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 1683
Size: 1419 264
Enzymes that do cut:
Acc65I AcuI AflIII AloI AlwNI ApoI AvaI BaeI
BanI BbsI BbvCI BciVI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstEII
BstXI BstYI Bsu36I BtsI ClaI DraI DraIII EaeI
Eco57MI EcoNI EcoRV FalI FspI Hin4I HincII KpnI
MfeI MmeI MscI MslI MspA1I NheI NspI PasI
PciI PflMI PfoI PpiI PstI PvuII SbfI ScaI
SfcI SmlI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanII BbeI BcgI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BsaBI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I BtgI BtgZI CspCI DrdI EagI EarI EciI
EcoICRI EcoO109I EcoRI FseI FspAI HaeII HindIII HpaI
KasI MluI NaeI NarI NcoI NdeI NgoMIV NotI
NruI NsiI PacI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XhoI XmaI XmnI ZraI