Restriction map for
pF1KB8295
(Linear) (Six-base) MAPSORT of: KIBB8295.seq Check: 5351 from: 1 to: 1419
KIBB8295.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 48 1419
Size: 48 1371
Acc65I G'GTAC_C
Cuts at: 0 155 1419
Size: 155 1264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 359 665 1173 1419
Size: 126 233 306 508 246
Fragments arranged by size:
508 306 246 233 126
AfeI AGC'GCT
Cuts at: 0 139 336 1419
Size: 139 197 1083
Fragments arranged by size:
1083 197 139
AflIII A'CryG_T
Cuts at: 0 914 1419
Size: 914 505
AgeI A'CCGG_T
Cuts at: 0 426 1419
Size: 426 993
AhdI GACnn_n'nnGTC
Cuts at: 0 397 1419
Size: 397 1022
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 1419
Size: 547 32 840
Fragments arranged by size:
840 547 32
AlwNI CAG_nnn'CTG
Cuts at: 0 252 279 457 1419
Size: 252 27 178 962
Fragments arranged by size:
962 252 178 27
ApaI G_GGCC'C
Cuts at: 0 40 530 1419
Size: 40 490 889
Fragments arranged by size:
889 490 40
ApaLI G'TGCA_C
Cuts at: 0 918 1419
Size: 918 501
AvaI C'yCGr_G
Cuts at: 0 176 226 471 1419
Size: 176 50 245 948
Fragments arranged by size:
948 245 176 50
AvrII C'CTAG_G
Cuts at: 0 40 1419
Size: 40 1379
BamHI G'GATC_C
Cuts at: 0 1139 1419
Size: 1139 280
BanI G'GyrC_C
Cuts at: 0 155 1071 1419
Size: 155 916 348
Fragments arranged by size:
916 348 155
BanII G_rGCy'C
Cuts at: 0 40 302 530 659 1033 1419
Size: 40 262 228 129 374 386
Fragments arranged by size:
386 374 262 228 129 40
BbeI G_GCGC'C
Cuts at: 0 1075 1419
Size: 1075 344
BbvCI CC'TCA_GC
Cuts at: 0 220 1207 1419
Size: 220 987 212
Fragments arranged by size:
987 220 212
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 496 1419
Size: 462 34 923
Fragments arranged by size:
923 462 34
BglII A'GATC_T
Cuts at: 0 363 1419
Size: 363 1056
Bme1580I G_kGCm'C
Cuts at: 0 40 530 922 1419
Size: 40 490 392 497
Fragments arranged by size:
497 490 392 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 908 1157 1419
Size: 22 886 249 262
Fragments arranged by size:
886 262 249 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 1419
Size: 789 630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 274 286 289 388 1419
Size: 142 132 12 3 99 1031
Fragments arranged by size:
1031 142 132 99 12 3
Bpu10I CC'TnA_GC
Cuts at: 0 187 220 1207 1419
Size: 187 33 987 212
Fragments arranged by size:
987 212 187 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 1419
Size: 554 865
BsaI GGTCTCn'nnnn_
Cuts at: 0 405 493 1419
Size: 405 88 926
Fragments arranged by size:
926 405 88
BsaAI yAC'GTr
Cuts at: 0 915 1419
Size: 915 504
BsaHI Gr'CG_yC
Cuts at: 0 1072 1419
Size: 1072 347
BsaWI w'CCGG_w
Cuts at: 0 426 1231 1419
Size: 426 805 188
Fragments arranged by size:
805 426 188
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1267 1297 1419
Size: 547 30 690 30 122
Fragments arranged by size:
690 547 122 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 346 398 438 568 1419
Size: 100 246 52 40 130 851
Fragments arranged by size:
851 246 130 100 52 40
BseYI C'CCAG_C
Cuts at: 0 608 1419
Size: 608 811
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1419
Size: 334 1085
BsiHKAI G_wGCw'C
Cuts at: 0 206 302 922 1033 1419
Size: 206 96 620 111 386
Fragments arranged by size:
620 386 206 111 96
BsmI GAATG_Cn'
Cuts at: 0 1061 1419
Size: 1061 358
BsmBI CGTCTCn'nnnn_
Cuts at: 0 816 1419
Size: 816 603
Bsp1286I G_dGCh'C
Cuts at: 0 40 206 302 530 659 922 1033 1419
Size: 40 166 96 228 129 263 111 386
Fragments arranged by size:
386 263 228 166 129 111 96 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 48 1419
Size: 48 1371
BsrBI CCG'CTC
Cuts at: 0 372 1419
Size: 372 1047
BsrDI GCAATG_nn'
Cuts at: 0 321 1419
Size: 321 1098
BsrFI r'CCGG_y
Cuts at: 0 426 989 1419
Size: 426 563 430
Fragments arranged by size:
563 430 426
BsrGI T'GTAC_A
Cuts at: 0 533 1419
Size: 533 886
BssSI C'ACGA_G
Cuts at: 0 613 1419
Size: 613 806
BstXI CCAn_nnnn'nTGG
Cuts at: 0 547 1419
Size: 547 872
BstYI r'GATC_y
Cuts at: 0 230 248 363 573 1139 1419
Size: 230 18 115 210 566 280
Fragments arranged by size:
566 280 230 210 115 18
Bsu36I CC'TnA_GG
Cuts at: 0 450 698 1419
Size: 450 248 721
Fragments arranged by size:
721 450 248
BtsI GCAGTG_nn'
Cuts at: 0 724 1419
Size: 724 695
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 476 511 1074 1109 1419
Size: 476 35 563 35 310
Fragments arranged by size:
563 476 310 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 926 984 1419
Size: 926 58 435
Fragments arranged by size:
926 435 58
EarI CTCTTCn'nnn_
Cuts at: 0 89 335 764 1061 1177 1419
Size: 89 246 429 297 116 242
Fragments arranged by size:
429 297 246 242 116 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 1419
Size: 121 1298
EcoICRI GAG'CTC
Cuts at: 0 300 1031 1419
Size: 300 731 388
Fragments arranged by size:
731 388 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 142 274 286 289 359 388 665
Size: 126 16 132 12 3 70 29 277
Cuts at: 665 1173 1419
Size: 508 246
Fragments arranged by size:
508 277 246 132 126 70 29 16
12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 812 1419
Size: 292 520 607
Fragments arranged by size:
607 520 292
EcoO109I rG'GnC_Cy
Cuts at: 0 37 64 183 526 527 1419
Size: 37 27 119 343 1 892
Fragments arranged by size:
892 343 119 37 27 1
EcoRV GAT'ATC
Cuts at: 0 443 1419
Size: 443 976
HaeII r_GCGC'y
Cuts at: 0 141 338 1075 1419
Size: 141 197 737 344
Fragments arranged by size:
737 344 197 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 427 459 1176 1208 1419
Size: 383 32 12 32 717 32 211
Fragments arranged by size:
717 383 211 32 32 32 12
HindIII A'AGCT_T
Cuts at: 0 935 1016 1419
Size: 935 81 403
Fragments arranged by size:
935 403 81
KasI G'GCGC_C
Cuts at: 0 1071 1419
Size: 1071 348
KpnI G_GTAC'C
Cuts at: 0 159 1419
Size: 159 1260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1419
Size: 1310 109
MslI CAynn'nnrTG
Cuts at: 0 1080 1248 1343 1419
Size: 1080 168 95 76
Fragments arranged by size:
1080 168 95 76
MspA1I CmG'CkG
Cuts at: 0 1109 1419
Size: 1109 310
NarI GG'CG_CC
Cuts at: 0 1072 1419
Size: 1072 347
NspI r_CATG'y
Cuts at: 0 483 1201 1419
Size: 483 718 218
Fragments arranged by size:
718 483 218
PfoI T'CCnGG_A
Cuts at: 0 251 1419
Size: 251 1168
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 779 811 1419
Size: 547 32 200 32 608
Fragments arranged by size:
608 547 200 32 32
PspOMI G'GGCC_C
Cuts at: 0 36 526 1419
Size: 36 490 893
Fragments arranged by size:
893 490 36
SacI G_AGCT'C
Cuts at: 0 302 1033 1419
Size: 302 731 386
Fragments arranged by size:
731 386 302
SapI GCTCTTCn'nnn_
Cuts at: 0 1177 1419
Size: 1177 242
SexAI A'CCwGG_T
Cuts at: 0 395 1419
Size: 395 1024
SfcI C'TryA_G
Cuts at: 0 969 1359 1419
Size: 969 390 60
Fragments arranged by size:
969 390 60
SfoI GGC'GCC
Cuts at: 0 1073 1419
Size: 1073 346
SmlI C'TyrA_G
Cuts at: 0 569 1419
Size: 569 850
SphI G_CATG'C
Cuts at: 0 483 1201 1419
Size: 483 718 218
Fragments arranged by size:
718 483 218
StuI AGG'CCT
Cuts at: 0 45 307 1419
Size: 45 262 1112
Fragments arranged by size:
1112 262 45
StyI C'CwwG_G
Cuts at: 0 8 40 239 494 1419
Size: 8 32 199 255 925
Fragments arranged by size:
925 255 199 32 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 300 1419
Size: 300 1119
TatI w'GTAC_w
Cuts at: 0 533 1419
Size: 533 886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 166 1419
Size: 166 1253
XmnI GAAnn'nnTTC
Cuts at: 0 794 937 1186 1270 1419
Size: 794 143 249 84 149
Fragments arranged by size:
794 249 149 143 84
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BcgI BglII Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtsI
CspCI DraIII EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV HaeII Hin4I HindIII KasI KpnI MmeI MslI
MspA1I NarI NspI PfoI PpiI PspOMI SacI SapI
SexAI SfcI SfoI SmlI SphI StuI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AleI AlfI ApoI AscI
AseI AsiSI BaeI BbsI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BsaBI BsiEI BsiWI BspEI BspHI
BssHII BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
DraI DrdI EaeI EagI EcoRI FalI FseI FspI
FspAI HincII HpaI MfeI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI