(Linear) (Six-base) MAPSORT of: KIBB8299.seq Check: 1480 from: 1 to: 1611 KIBB8299.orf With 182 enzymes: SgfI * March 3, 2008 11:04 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 632 1000 1075 1611 Size: 632 368 75 536 Fragments arranged by size: 632 536 368 75 AleI CACnn'nnGTG Cuts at: 0 529 1177 1611 Size: 529 648 434 Fragments arranged by size: 648 529 434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 294 388 547 1611 Size: 294 94 159 1064 Fragments arranged by size: 1064 294 159 94 AlwNI CAG_nnn'CTG Cuts at: 0 264 399 543 555 1086 1611 Size: 264 135 144 12 531 525 Fragments arranged by size: 531 525 264 144 135 12 AvaI C'yCGr_G Cuts at: 0 8 221 1309 1526 1611 Size: 8 213 1088 217 85 Fragments arranged by size: 1088 217 213 85 8 BanII G_rGCy'C Cuts at: 0 8 1611 Size: 8 1603 BbsI GAAGACnn'nnnn_ Cuts at: 0 734 1611 Size: 734 877 BlpI GC'TnA_GC Cuts at: 0 376 1003 1611 Size: 376 627 608 Fragments arranged by size: 627 608 376 Bme1580I G_kGCm'C Cuts at: 0 936 1026 1232 1611 Size: 936 90 206 379 Fragments arranged by size: 936 379 206 90 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1010 1611 Size: 1010 601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 607 812 898 1473 1611 Size: 364 243 205 86 575 138 Fragments arranged by size: 575 364 243 205 138 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 835 1611 Size: 337 498 776 Fragments arranged by size: 776 498 337 BsaI GGTCTCn'nnnn_ Cuts at: 0 156 788 1611 Size: 156 632 823 Fragments arranged by size: 823 632 156 BsaWI w'CCGG_w Cuts at: 0 125 1611 Size: 125 1486 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 617 647 1471 1501 1611 Size: 617 30 824 30 110 Fragments arranged by size: 824 617 110 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 474 490 820 859 928 1414 1450 Size: 55 419 16 330 39 69 486 36 Cuts at: 1450 1575 1611 Size: 125 36 Fragments arranged by size: 486 419 330 125 69 55 39 36 36 16 BseYI C'CCAG_C Cuts at: 0 597 1038 1611 Size: 597 441 573 Fragments arranged by size: 597 573 441 BsiHKAI G_wGCw'C Cuts at: 0 86 377 683 1219 1451 1611 Size: 86 291 306 536 232 160 Fragments arranged by size: 536 306 291 232 160 86 BsmI GAATG_Cn' Cuts at: 0 1266 1611 Size: 1266 345 BsmBI CGTCTCn'nnnn_ Cuts at: 0 25 1611 Size: 25 1586 Bsp1286I G_dGCh'C Cuts at: 0 8 86 377 683 936 1026 1219 1232 Size: 8 78 291 306 253 90 193 13 Cuts at: 1232 1451 1611 Size: 219 160 Fragments arranged by size: 306 291 253 219 193 160 90 78 13 8 BspHI T'CATG_A Cuts at: 0 1157 1611 Size: 1157 454 BsrBI CCG'CTC Cuts at: 0 657 1611 Size: 657 954 BsrFI r'CCGG_y Cuts at: 0 1304 1611 Size: 1304 307 BssSI C'ACGA_G Cuts at: 0 370 1450 1611 Size: 370 1080 161 Fragments arranged by size: 1080 370 161 BstEII G'GTnAC_C Cuts at: 0 1467 1611 Size: 1467 144 BstXI CCAn_nnnn'nTGG Cuts at: 0 1464 1611 Size: 1464 147 BstYI r'GATC_y Cuts at: 0 668 1611 Size: 668 943 Bsu36I CC'TnA_GG Cuts at: 0 1118 1611 Size: 1118 493 BtgI C'CryG_G Cuts at: 0 26 662 813 896 1611 Size: 26 636 151 83 715 Fragments arranged by size: 715 636 151 83 26 BtsI GCAGTG_nn' Cuts at: 0 830 1611 Size: 830 781 DraIII CAC_nnn'GTG Cuts at: 0 774 825 1611 Size: 774 51 786 Fragments arranged by size: 786 774 51 DrdI GACnn_nn'nnGTC Cuts at: 0 295 1611 Size: 295 1316 EaeI y'GGCC_r Cuts at: 0 816 1069 1611 Size: 816 253 542 Fragments arranged by size: 816 542 253 EarI CTCTTCn'nnn_ Cuts at: 0 124 508 688 1177 1611 Size: 124 384 180 489 434 Fragments arranged by size: 489 434 384 180 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 154 1141 1611 Size: 154 987 470 Fragments arranged by size: 987 470 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 364 607 632 812 898 1000 1075 1473 Size: 364 243 25 180 86 102 75 398 Cuts at: 1473 1611 Size: 138 Fragments arranged by size: 398 364 243 180 138 102 86 75 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 163 1611 Size: 163 1448 EcoO109I rG'GnC_Cy Cuts at: 0 755 1287 1611 Size: 755 532 324 Fragments arranged by size: 755 532 324 FseI GG_CCGG'CC Cuts at: 0 1308 1611 Size: 1308 303 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1471 1503 1582 1611 Size: 1471 32 79 29 Fragments arranged by size: 1471 79 32 29 HindIII A'AGCT_T Cuts at: 0 1144 1611 Size: 1144 467 MscI TGG'CCA Cuts at: 0 1071 1611 Size: 1071 540 MslI CAynn'nnrTG Cuts at: 0 529 910 1177 1611 Size: 529 381 267 434 Fragments arranged by size: 529 434 381 267 MspA1I CmG'CkG Cuts at: 0 543 1611 Size: 543 1068 NaeI GCC'GGC Cuts at: 0 1306 1611 Size: 1306 305 NcoI C'CATG_G Cuts at: 0 662 1611 Size: 662 949 NgoMIV G'CCGG_C Cuts at: 0 1304 1611 Size: 1304 307 PasI CC'CwG_GG Cuts at: 0 1062 1611 Size: 1062 549 PflMI CCAn_nnn'nTGG Cuts at: 0 1512 1611 Size: 1512 99 PfoI T'CCnGG_A Cuts at: 0 158 1489 1611 Size: 158 1331 122 Fragments arranged by size: 1331 158 122 PpuMI rG'GwC_Cy Cuts at: 0 755 1611 Size: 755 856 PstI C_TGCA'G Cuts at: 0 203 269 1550 1611 Size: 203 66 1281 61 Fragments arranged by size: 1281 203 66 61 PvuII CAG'CTG Cuts at: 0 543 1611 Size: 543 1068 SanDI GG'GwC_CC Cuts at: 0 755 1611 Size: 755 856 SapI GCTCTTCn'nnn_ Cuts at: 0 688 1611 Size: 688 923 SbfI CC_TGCA'GG Cuts at: 0 269 1550 1611 Size: 269 1281 61 Fragments arranged by size: 1281 269 61 ScaI AGT'ACT Cuts at: 0 1321 1611 Size: 1321 290 SfcI C'TryA_G Cuts at: 0 199 265 1546 1611 Size: 199 66 1281 65 Fragments arranged by size: 1281 199 66 65 SmaI CCC'GGG Cuts at: 0 10 223 1528 1611 Size: 10 213 1305 83 Fragments arranged by size: 1305 213 83 10 SmlI C'TyrA_G Cuts at: 0 352 850 1611 Size: 352 498 761 Fragments arranged by size: 761 498 352 StyI C'CwwG_G Cuts at: 0 662 1611 Size: 662 949 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 642 1611 Size: 642 969 TatI w'GTAC_w Cuts at: 0 339 1244 1319 1352 1611 Size: 339 905 75 33 259 Fragments arranged by size: 905 339 259 75 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 617 621 649 653 1543 1575 1611 Size: 617 4 28 4 890 32 36 Fragments arranged by size: 890 617 36 32 28 4 4 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 903 1290 1611 Size: 903 387 321 Fragments arranged by size: 903 387 321 XmaI C'CCGG_G Cuts at: 0 8 221 1526 1611 Size: 8 213 1305 85 Fragments arranged by size: 1305 213 85 8 Enzymes that do cut: AcuI AleI AlfI AlwNI AvaI BanII BbsI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FseI Hin4I HindIII MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PstI PvuII SanDI SapI SbfI ScaI SfcI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BspEI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FalI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI