(Linear) (Six-base) MAPSORT of: KIBB8300.seq Check: 3715 from: 1 to: 1389 KIBB8300.orf With 182 enzymes: SgfI * March 3, 2008 11:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1254 1389 Size: 1254 135 AatII G_ACGT'C Cuts at: 0 746 1192 1389 Size: 746 446 197 Fragments arranged by size: 746 446 197 AccI GT'mk_AC Cuts at: 0 1286 1389 Size: 1286 103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 845 1389 Size: 845 544 AlwNI CAG_nnn'CTG Cuts at: 0 279 1389 Size: 279 1110 ApaI G_GGCC'C Cuts at: 0 286 945 1389 Size: 286 659 444 Fragments arranged by size: 659 444 286 ApaLI G'TGCA_C Cuts at: 0 715 1389 Size: 715 674 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1244 1277 1389 Size: 1244 33 112 Fragments arranged by size: 1244 112 33 BanI G'GyrC_C Cuts at: 0 67 523 960 1009 1389 Size: 67 456 437 49 380 Fragments arranged by size: 456 437 380 67 49 BanII G_rGCy'C Cuts at: 0 286 466 658 695 945 1027 1389 Size: 286 180 192 37 250 82 362 Fragments arranged by size: 362 286 250 192 180 82 37 BbeI G_GCGC'C Cuts at: 0 964 1389 Size: 964 425 BbsI GAAGACnn'nnnn_ Cuts at: 0 253 1389 Size: 253 1136 BclI T'GATC_A Cuts at: 0 575 971 1389 Size: 575 396 418 Fragments arranged by size: 575 418 396 BglI GCCn_nnn'nGGC Cuts at: 0 211 236 838 1031 1389 Size: 211 25 602 193 358 Fragments arranged by size: 602 358 211 193 25 BglII A'GATC_T Cuts at: 0 602 1389 Size: 602 787 Bme1580I G_kGCm'C Cuts at: 0 72 241 286 526 682 719 945 1012 Size: 72 169 45 240 156 37 226 67 Cuts at: 1012 1389 Size: 377 Fragments arranged by size: 377 240 226 169 156 72 67 45 37 BmgBI CAC'GTC Cuts at: 0 573 1389 Size: 573 816 BmrI ACTGGGnnnn_n' Cuts at: 0 100 1389 Size: 100 1289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 942 1329 1389 Size: 942 387 60 Fragments arranged by size: 942 387 60 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 352 679 899 967 1204 1389 Size: 199 153 327 220 68 237 185 Fragments arranged by size: 327 237 220 199 185 153 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 1389 Size: 472 917 BsaI GGTCTCn'nnnn_ Cuts at: 0 499 1366 1389 Size: 499 867 23 Fragments arranged by size: 867 499 23 BsaBI GATnn'nnATC Cuts at: 0 580 1389 Size: 580 809 BsaHI Gr'CG_yC Cuts at: 0 743 961 1154 1189 1343 1389 Size: 743 218 193 35 154 46 Fragments arranged by size: 743 218 193 154 46 35 BsaWI w'CCGG_w Cuts at: 0 1118 1389 Size: 1118 271 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 790 820 1389 Size: 790 30 569 Fragments arranged by size: 790 569 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 412 868 1389 Size: 412 456 521 Fragments arranged by size: 521 456 412 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 715 1282 1389 Size: 694 21 567 107 Fragments arranged by size: 694 567 107 21 BsiEI CG_ry'CG Cuts at: 0 210 1389 Size: 210 1179 BsiHKAI G_wGCw'C Cuts at: 0 695 719 1389 Size: 695 24 670 Fragments arranged by size: 695 670 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 394 1074 1180 1389 Size: 394 680 106 209 Fragments arranged by size: 680 394 209 106 Bsp1286I G_dGCh'C Cuts at: 0 72 241 286 466 526 658 682 695 Size: 72 169 45 180 60 132 24 13 Cuts at: 695 719 945 1012 1027 1389 Size: 24 226 67 15 362 Fragments arranged by size: 362 226 180 169 132 72 67 60 45 24 24 15 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1055 1254 1389 Size: 1055 199 135 Fragments arranged by size: 1055 199 135 BsrBI CCG'CTC Cuts at: 0 620 1313 1389 Size: 620 693 76 Fragments arranged by size: 693 620 76 BsrFI r'CCGG_y Cuts at: 0 205 310 1055 1389 Size: 205 105 745 334 Fragments arranged by size: 745 334 205 105 BssSI C'ACGA_G Cuts at: 0 733 1389 Size: 733 656 BstAPI GCAn_nnn'nTGC Cuts at: 0 1121 1389 Size: 1121 268 BstXI CCAn_nnnn'nTGG Cuts at: 0 261 267 501 1389 Size: 261 6 234 888 Fragments arranged by size: 888 261 234 6 BstYI r'GATC_y Cuts at: 0 35 431 602 1106 1389 Size: 35 396 171 504 283 Fragments arranged by size: 504 396 283 171 35 BtgI C'CryG_G Cuts at: 0 260 987 1376 1389 Size: 260 727 389 13 Fragments arranged by size: 727 389 260 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 240 291 1389 Size: 240 51 1098 Fragments arranged by size: 1098 240 51 BtsI GCAGTG_nn' Cuts at: 0 321 682 1192 1389 Size: 321 361 510 197 Fragments arranged by size: 510 361 321 197 DraIII CAC_nnn'GTG Cuts at: 0 723 1389 Size: 723 666 EaeI y'GGCC_r Cuts at: 0 228 251 977 1389 Size: 228 23 726 412 Fragments arranged by size: 726 412 228 23 EarI CTCTTCn'nnn_ Cuts at: 0 784 1300 1357 1389 Size: 784 516 57 32 Fragments arranged by size: 784 516 57 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 880 1389 Size: 880 509 EcoICRI GAG'CTC Cuts at: 0 693 1389 Size: 693 696 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 352 679 899 967 1204 1389 Size: 199 153 327 220 68 237 185 Fragments arranged by size: 327 237 220 199 185 153 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 547 1389 Size: 547 842 EcoO109I rG'GnC_Cy Cuts at: 0 276 283 942 1389 Size: 276 7 659 447 Fragments arranged by size: 659 447 276 7 HaeII r_GCGC'y Cuts at: 0 355 778 964 1389 Size: 355 423 186 425 Fragments arranged by size: 425 423 355 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 192 224 790 822 1389 Size: 192 32 566 32 567 Fragments arranged by size: 567 566 192 32 32 KasI G'GCGC_C Cuts at: 0 960 1389 Size: 960 429 MscI TGG'CCA Cuts at: 0 230 979 1389 Size: 230 749 410 Fragments arranged by size: 749 410 230 MslI CAynn'nnrTG Cuts at: 0 259 265 499 529 1389 Size: 259 6 234 30 860 Fragments arranged by size: 860 259 234 30 6 MspA1I CmG'CkG Cuts at: 0 147 1222 1389 Size: 147 1075 167 Fragments arranged by size: 1075 167 147 NaeI GCC'GGC Cuts at: 0 312 1389 Size: 312 1077 NarI GG'CG_CC Cuts at: 0 961 1389 Size: 961 428 NcoI C'CATG_G Cuts at: 0 260 1376 1389 Size: 260 1116 13 Fragments arranged by size: 1116 260 13 NgoMIV G'CCGG_C Cuts at: 0 310 1389 Size: 310 1079 NspI r_CATG'y Cuts at: 0 909 1389 Size: 909 480 PasI CC'CwG_GG Cuts at: 0 279 762 1389 Size: 279 483 627 Fragments arranged by size: 627 483 279 PflMI CCAn_nnn'nTGG Cuts at: 0 909 1389 Size: 909 480 PfoI T'CCnGG_A Cuts at: 0 29 188 1298 1389 Size: 29 159 1110 91 Fragments arranged by size: 1110 159 91 29 PspOMI G'GGCC_C Cuts at: 0 282 941 1389 Size: 282 659 448 Fragments arranged by size: 659 448 282 PvuII CAG'CTG Cuts at: 0 147 1222 1389 Size: 147 1075 167 Fragments arranged by size: 1075 167 147 SacI G_AGCT'C Cuts at: 0 695 1389 Size: 695 694 SapI GCTCTTCn'nnn_ Cuts at: 0 784 1389 Size: 784 605 SfcI C'TryA_G Cuts at: 0 1083 1389 Size: 1083 306 SfoI GGC'GCC Cuts at: 0 962 1389 Size: 962 427 SmlI C'TyrA_G Cuts at: 0 487 1389 Size: 487 902 StuI AGG'CCT Cuts at: 0 364 1389 Size: 364 1025 StyI C'CwwG_G Cuts at: 0 107 260 467 548 833 1376 1389 Size: 107 153 207 81 285 543 13 Fragments arranged by size: 543 285 207 153 107 81 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 361 775 1389 Size: 361 414 614 Fragments arranged by size: 614 414 361 TatI w'GTAC_w Cuts at: 0 1322 1389 Size: 1322 67 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 709 741 1389 Size: 709 32 648 Fragments arranged by size: 709 648 32 Tth111I GACn'n_nGTC Cuts at: 0 556 898 1389 Size: 556 342 491 Fragments arranged by size: 556 491 342 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 516 1047 1389 Size: 516 531 342 Fragments arranged by size: 531 516 342 ZraI GAC'GTC Cuts at: 0 744 1190 1389 Size: 744 446 199 Fragments arranged by size: 744 446 199 Enzymes that do cut: AarI AatII AccI AlfI AlwNI ApaI ApaLI BaeI BanI BanII BbeI BbsI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PspOMI PvuII SacI SapI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI BfrBI BlpI BmtI Bpu10I BsaAI BseYI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI