Restriction map for
pF1KB8303
(Linear) (Six-base) MAPSORT of: KIBB8303.seq Check: 429 from: 1 to: 1800
KIBB8303.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1116 1137 1543 1556 1800
Size: 1116 21 406 13 244
Fragments arranged by size:
1116 406 244 21 13
AccI GT'mk_AC
Cuts at: 0 335 521 1589 1800
Size: 335 186 1068 211
Fragments arranged by size:
1068 335 211 186
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 685 802 1288 1402 1633 1792 1800
Size: 222 463 117 486 114 231 159 8
Fragments arranged by size:
486 463 231 222 159 117 114 8
AfeI AGC'GCT
Cuts at: 0 643 1800
Size: 643 1157
AflIII A'CryG_T
Cuts at: 0 893 1157 1800
Size: 893 264 643
Fragments arranged by size:
893 643 264
AleI CACnn'nnGTG
Cuts at: 0 1708 1800
Size: 1708 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1726 1800
Size: 1726 74
AlwNI CAG_nnn'CTG
Cuts at: 0 514 999 1131 1404 1800
Size: 514 485 132 273 396
Fragments arranged by size:
514 485 396 273 132
ApaLI G'TGCA_C
Cuts at: 0 1543 1800
Size: 1543 257
AvaI C'yCGr_G
Cuts at: 0 1108 1800
Size: 1108 692
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 700 733 1800
Size: 700 33 1067
Fragments arranged by size:
1067 700 33
BanI G'GyrC_C
Cuts at: 0 786 1069 1441 1702 1800
Size: 786 283 372 261 98
Fragments arranged by size:
786 372 283 261 98
BanII G_rGCy'C
Cuts at: 0 508 743 1292 1800
Size: 508 235 549 508
Fragments arranged by size:
549 508 508 235
BbvCI CC'TCA_GC
Cuts at: 0 1090 1800
Size: 1090 710
BclI T'GATC_A
Cuts at: 0 191 374 1448 1800
Size: 191 183 1074 352
Fragments arranged by size:
1074 352 191 183
BglII A'GATC_T
Cuts at: 0 1004 1800
Size: 1004 796
BlpI GC'TnA_GC
Cuts at: 0 1514 1800
Size: 1514 286
Bme1580I G_kGCm'C
Cuts at: 0 228 791 1072 1547 1800
Size: 228 563 281 475 253
Fragments arranged by size:
563 475 281 253 228
BmgBI CAC'GTC
Cuts at: 0 42 1800
Size: 42 1758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 463 775 1153 1657 1800
Size: 331 132 312 378 504 143
Fragments arranged by size:
504 378 331 312 143 132
Bpu10I CC'TnA_GC
Cuts at: 0 1090 1800
Size: 1090 710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1800
Size: 1585 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 673 751 1725 1800
Size: 69 604 78 974 75
Fragments arranged by size:
974 604 78 75 69
BsaAI yAC'GTr
Cuts at: 0 471 614 1800
Size: 471 143 1186
Fragments arranged by size:
1186 471 143
BsaHI Gr'CG_yC
Cuts at: 0 1181 1800
Size: 1181 619
BsaWI w'CCGG_w
Cuts at: 0 1010 1800
Size: 1010 790
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1144 1174 1800
Size: 1144 30 626
Fragments arranged by size:
1144 626 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1660 1756 1800
Size: 1660 96 44
Fragments arranged by size:
1660 96 44
BseYI C'CCAG_C
Cuts at: 0 474 723 988 1348 1371 1503 1746 1795
Size: 474 249 265 360 23 132 243 49
Cuts at: 1795 1800
Size: 5
Fragments arranged by size:
474 360 265 249 243 132 49 23
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 967 1084 1420 1648 1800
Size: 904 63 117 336 228 152
Fragments arranged by size:
904 336 228 152 117 63
BsiEI CG_ry'CG
Cuts at: 0 126 165 927 1800
Size: 126 39 762 873
Fragments arranged by size:
873 762 126 39
BsiHKAI G_wGCw'C
Cuts at: 0 743 1292 1520 1547 1800
Size: 743 549 228 27 253
Fragments arranged by size:
743 549 253 228 27
BsiWI C'GTAC_G
Cuts at: 0 614 1800
Size: 614 1186
BsmI GAATG_Cn'
Cuts at: 0 107 983 1800
Size: 107 876 817
Fragments arranged by size:
876 817 107
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1318 1567 1800
Size: 1318 249 233
Fragments arranged by size:
1318 249 233
Bsp1286I G_dGCh'C
Cuts at: 0 228 508 743 791 1072 1292 1520 1547
Size: 228 280 235 48 281 220 228 27
Cuts at: 1547 1800
Size: 253
Fragments arranged by size:
281 280 253 235 228 228 220 48
27
BspEI T'CCGG_A
Cuts at: 0 1010 1800
Size: 1010 790
BspHI T'CATG_A
Cuts at: 0 1451 1800
Size: 1451 349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 1116 1137 1543 1556 1800
Size: 602 514 21 406 13 244
Fragments arranged by size:
602 514 406 244 21 13
BsrDI GCAATG_nn'
Cuts at: 0 68 1579 1800
Size: 68 1511 221
Fragments arranged by size:
1511 221 68
BsrFI r'CCGG_y
Cuts at: 0 31 1311 1800
Size: 31 1280 489
Fragments arranged by size:
1280 489 31
BssSI C'ACGA_G
Cuts at: 0 676 1800
Size: 676 1124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 999 1404 1524 1800
Size: 999 405 120 276
Fragments arranged by size:
999 405 276 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 309 1257 1800
Size: 309 948 543
Fragments arranged by size:
948 543 309
BstYI r'GATC_y
Cuts at: 0 866 1004 1085 1800
Size: 866 138 81 715
Fragments arranged by size:
866 715 138 81
Bsu36I CC'TnA_GG
Cuts at: 0 1412 1800
Size: 1412 388
BtgI C'CryG_G
Cuts at: 0 683 803 1800
Size: 683 120 997
Fragments arranged by size:
997 683 120
BtsI GCAGTG_nn'
Cuts at: 0 216 1800
Size: 216 1584
DraIII CAC_nnn'GTG
Cuts at: 0 1626 1800
Size: 1626 174
DrdI GACnn_nn'nnGTC
Cuts at: 0 1659 1800
Size: 1659 141
EaeI y'GGCC_r
Cuts at: 0 33 162 1077 1313 1800
Size: 33 129 915 236 487
Fragments arranged by size:
915 487 236 129 33
EagI C'GGCC_G
Cuts at: 0 162 1800
Size: 162 1638
EcoICRI GAG'CTC
Cuts at: 0 741 1290 1800
Size: 741 549 510
Fragments arranged by size:
741 549 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 331 463 685 775 802 1153 1288
Size: 222 109 132 222 90 27 351 135
Cuts at: 1288 1402 1633 1657 1792 1800
Size: 114 231 24 135 8
Fragments arranged by size:
351 231 222 222 135 135 132 114
109 90 27 24 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1672 1786 1800
Size: 1672 114 14
Fragments arranged by size:
1672 114 14
EcoO109I rG'GnC_Cy
Cuts at: 0 1695 1779 1791 1800
Size: 1695 84 12 9
Fragments arranged by size:
1695 84 12 9
FseI GG_CCGG'CC
Cuts at: 0 35 1800
Size: 35 1765
HaeII r_GCGC'y
Cuts at: 0 645 1800
Size: 645 1155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1800
Size: 1144 32 624
Fragments arranged by size:
1144 624 32
HincII GTy'rAC
Cuts at: 0 1299 1800
Size: 1299 501
MslI CAynn'nnrTG
Cuts at: 0 271 1550 1708 1800
Size: 271 1279 158 92
Fragments arranged by size:
1279 271 158 92
MspA1I CmG'CkG
Cuts at: 0 1094 1101 1200 1524 1800
Size: 1094 7 99 324 276
Fragments arranged by size:
1094 324 276 99 7
NaeI GCC'GGC
Cuts at: 0 33 1313 1800
Size: 33 1280 487
Fragments arranged by size:
1280 487 33
NgoMIV G'CCGG_C
Cuts at: 0 31 1311 1800
Size: 31 1280 489
Fragments arranged by size:
1280 489 31
NspI r_CATG'y
Cuts at: 0 897 1161 1311 1800
Size: 897 264 150 489
Fragments arranged by size:
897 489 264 150
PciI A'CATG_T
Cuts at: 0 893 1157 1800
Size: 893 264 643
Fragments arranged by size:
893 643 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 474 1800
Size: 42 432 1326
Fragments arranged by size:
1326 432 42
PfoI T'CCnGG_A
Cuts at: 0 179 752 1637 1723 1800
Size: 179 573 885 86 77
Fragments arranged by size:
885 573 179 86 77
PmlI CAC'GTG
Cuts at: 0 471 1800
Size: 471 1329
PpuMI rG'GwC_Cy
Cuts at: 0 1695 1800
Size: 1695 105
PsiI TTA'TAA
Cuts at: 0 536 1800
Size: 536 1264
PstI C_TGCA'G
Cuts at: 0 1004 1800
Size: 1004 796
PvuII CAG'CTG
Cuts at: 0 1200 1524 1800
Size: 1200 324 276
Fragments arranged by size:
1200 324 276
SacI G_AGCT'C
Cuts at: 0 743 1292 1800
Size: 743 549 508
Fragments arranged by size:
743 549 508
SfcI C'TryA_G
Cuts at: 0 1000 1590 1800
Size: 1000 590 210
Fragments arranged by size:
1000 590 210
SmlI C'TyrA_G
Cuts at: 0 1108 1600 1800
Size: 1108 492 200
Fragments arranged by size:
1108 492 200
StuI AGG'CCT
Cuts at: 0 454 919 1800
Size: 454 465 881
Fragments arranged by size:
881 465 454
StyI C'CwwG_G
Cuts at: 0 23 1184 1706 1800
Size: 23 1161 522 94
Fragments arranged by size:
1161 522 94 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 140 169 301 566 829 966 1800
Size: 140 29 132 265 263 137 834
Fragments arranged by size:
834 265 263 140 137 132 29
TatI w'GTAC_w
Cuts at: 0 1154 1800
Size: 1154 646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1713 1800
Size: 1713 87
XhoI C'TCGA_G
Cuts at: 0 1108 1800
Size: 1108 692
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AlwNI
ApaLI AvaI BaeI BanI BanII BbvCI BclI BglII
BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I
FseI HaeII Hin4I HincII MslI MspA1I NaeI NgoMIV
NspI PciI PflMI PfoI PmlI PpuMI PsiI PstI
PvuII SacI SfcI SmlI StuI StyI TaqII TatI
XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AloI ApaI
ApoI AscI AseI AsiSI AvrII BamHI BbeI BbsI
BcgI BciVI BfrBI BglI BmrI BmtI BplI BsaBI
BsrBI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI EarI EciI EcoRI EcoRV FalI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XmaI
XmnI ZraI