(Linear) (Six-base) MAPSORT of: KIBB8306.seq  Check: 8656  from: 1  to: 426



KIBB8306.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

Acc65I G'GTAC_C

Cuts at:      0      18     426 
   Size:         18     408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     426 
   Size:        104     322
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135     426 
   Size:        135     291
 
ApoI r'AATT_y

Cuts at:      0     316     426 
   Size:        316     110
 
BanI G'GyrC_C

Cuts at:      0      18     426 
   Size:         18     408
 
BanII G_rGCy'C

Cuts at:      0     257     421     426 
   Size:        257     164       5

  Fragments arranged by size: 

                 257     164       5
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      92     426 
   Size:         92     334
 
BclI T'GATC_A

Cuts at:      0       2     426 
   Size:          2     424
 
BlpI GC'TnA_GC

Cuts at:      0     302     426 
   Size:        302     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     354     426 
   Size:        354      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     426 
   Size:        403      23
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     256     426 
   Size:        229      27     170

  Fragments arranged by size: 

                 229     170      27
 
BsaHI Gr'CG_yC

Cuts at:      0      62     426 
   Size:         62     364
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     349     426 
   Size:        298      51      77

  Fragments arranged by size: 

                 298      77      51
 
BsiHKAI G_wGCw'C

Cuts at:      0     257     426 
   Size:        257     169
 
Bsp1286I G_dGCh'C

Cuts at:      0     257     421     426 
   Size:        257     164       5

  Fragments arranged by size: 

                 257     164       5
 
BspHI T'CATG_A

Cuts at:      0     147     426 
   Size:        147     279
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      30     426 
   Size:         30     396
 
BsrDI GCAATG_nn'

Cuts at:      0     257     426 
   Size:        257     169
 
BstBI TT'CG_AA

Cuts at:      0     393     426 
   Size:        393      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      99     426 
   Size:         99     327
 
BtgI C'CryG_G

Cuts at:      0     164     426 
   Size:        164     262
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      27     426 
   Size:         27     399
 
BtsI GCAGTG_nn'

Cuts at:      0     183     426 
   Size:        183     243
 
EarI CTCTTCn'nnn_

Cuts at:      0     275     403     426 
   Size:        275     128      23

  Fragments arranged by size: 

                 275     128      23
 
EcoICRI GAG'CTC

Cuts at:      0     255     426 
   Size:        255     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     403     426 
   Size:        104     299      23

  Fragments arranged by size: 

                 299     104      23
 
EcoRI G'AATT_C

Cuts at:      0     316     426 
   Size:        316     110
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     223     426 
   Size:        223     203
 
KpnI G_GTAC'C

Cuts at:      0      22     426 
   Size:         22     404
 
MslI CAynn'nnrTG

Cuts at:      0     169     426 
   Size:        169     257
 
NcoI C'CATG_G

Cuts at:      0     164     426 
   Size:        164     262
 
SacI G_AGCT'C

Cuts at:      0     257     426 
   Size:        257     169
 
SfcI C'TryA_G

Cuts at:      0     127     426 
   Size:        127     299
 
SmlI C'TyrA_G

Cuts at:      0     208     235     426 
   Size:        208      27     191

  Fragments arranged by size: 

                 208     191      27
 
StyI C'CwwG_G

Cuts at:      0     164     426 
   Size:        164     262
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI    BanII     BbsI     BclI
     BlpI     BmrI     BpmI    BpuEI    BsaHI    BseRI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrDI    BstBI    BstXI     BtgI    BtgZI     BtsI
     EarI  EcoICRI  Eco57MI    EcoRI     FalI     KpnI     MslI     NcoI
     SacI     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI