(Linear) (Six-base) MAPSORT of: KIBB8307.seq  Check: 4951  from: 1  to: 354



KIBB8307.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

ApaI G_GGCC'C

Cuts at:      0      27     354 
   Size:         27     327
 
ApoI r'AATT_y

Cuts at:      0     154     354 
   Size:        154     200
 
AseI AT'TA_AT

Cuts at:      0     182     354 
   Size:        182     172
 
BanII G_rGCy'C

Cuts at:      0      27     354 
   Size:         27     327
 
Bme1580I G_kGCm'C

Cuts at:      0      27     354 
   Size:         27     327
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     334     354 
   Size:        173     161      20

  Fragments arranged by size: 

                 173     161      20
 
BsaBI GATnn'nnATC

Cuts at:      0     197     354 
   Size:        197     157
 
BsaWI w'CCGG_w

Cuts at:      0      86     354 
   Size:         86     268
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       4      34      65      95     354 
   Size:          4      30      31      30     259

  Fragments arranged by size: 

                 259      31      30      30       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     200     354 
   Size:        200     154
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     354 
   Size:         27     327
 
EaeI y'GGCC_r

Cuts at:      0      65     354 
   Size:         65     289
 
EarI CTCTTCn'nnn_

Cuts at:      0     119     218     350     354 
   Size:        119      99     132       4

  Fragments arranged by size: 

                 132     119      99       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     334     354 
   Size:        173     161      20

  Fragments arranged by size: 

                 173     161      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     354 
   Size:         24     330
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      65      97     354 
   Size:         65      32     257

  Fragments arranged by size: 

                 257      65      32
 
HincII GTy'rAC

Cuts at:      0      84     354 
   Size:         84     270
 
MscI TGG'CCA

Cuts at:      0      67     354 
   Size:         67     287
 
PfoI T'CCnGG_A

Cuts at:      0     149     354 
   Size:        149     205
 
PspOMI G'GGCC_C

Cuts at:      0      23     354 
   Size:         23     331
 
SapI GCTCTTCn'nnn_

Cuts at:      0     350     354 
   Size:        350       4
 
StyI C'CwwG_G

Cuts at:      0      68     354 
   Size:         68     286
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       4      36     354 
   Size:          4      32     318

  Fragments arranged by size: 

                 318      32       4
 

 Enzymes that do cut:

     ApaI     ApoI     AseI    BanII Bme1580I     BpmI    BsaBI    BsaWI
    BsaXI    BseRI Bsp1286I     EaeI     EarI  Eco57MI EcoO109I    Hin4I
   HincII     MscI     PfoI   PspOMI     SapI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI