(Linear) (Six-base) MAPSORT of: KIBB8309.seq  Check: 2458  from: 1  to: 204



KIBB8309.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

BamHI G'GATC_C

Cuts at:      0      12     204 
   Size:         12     192
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     134     204 
   Size:        134      70
 
BglII A'GATC_T

Cuts at:      0     136     204 
   Size:        136      68
 
BsiHKAI G_wGCw'C

Cuts at:      0     120     204 
   Size:        120      84
 
Bsp1286I G_dGCh'C

Cuts at:      0     120     204 
   Size:        120      84
 
BstYI r'GATC_y

Cuts at:      0      12     136     204 
   Size:         12     124      68

  Fragments arranged by size: 

                 124      68      12
 
EarI CTCTTCn'nnn_

Cuts at:      0     161     204 
   Size:        161      43
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     128     160     204 
   Size:        128      32      44

  Fragments arranged by size: 

                 128      44      32
 
StyI C'CwwG_G

Cuts at:      0     128     204 
   Size:        128      76
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      64      96     204 
   Size:         64      32     108

  Fragments arranged by size: 

                 108      64      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      66     204 
   Size:         66     138
 

 Enzymes that do cut:

    BamHI    BciVI    BglII  BsiHKAI Bsp1286I    BstYI     EarI    Hin4I
     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI